{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T15:09:24Z","timestamp":1761664164748},"reference-count":31,"publisher":"Georg Thieme Verlag KG","issue":"06","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tierarztl Prax Ausg K Kleintiere Heimtiere"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Zusammenfassung<\/jats:title><jats:p>\n               Gegenstand und Ziel Kreuzreagierende Kohlenhydrat-Seitenketten (cross-reactive carbohydrate determinants, CCD) f\u00fchren bei In-vitro-Allergietests zum Nachweis von IgE gegen saisonale Allergene zu polypositiven Ergebnissen. Falsch-positive\/klinisch irrelevante Resultate aufgrund der Bindung von Immunglobulin IgE an CCD (Anti-CCD-IgE) wurden bei Pollenallergenen identifiziert. Bisher fehlten Studien \u00fcber das Ph\u00e4nomen bei Katzen. Das Ziel dieser Studie war, die Pr\u00e4valenz der Polysensibilisierung in Serumproben und die Auswirkung eines CCD-Inhibitors\/Blockers bei multipositiven, saisonalen Allergietestergebnissen bei Katzen zu evaluieren.<\/jats:p><jats:p>\n               Material und Methoden Insgesamt wurden 472\u2005Katzenseren ber\u00fccksichtigt, die f\u00fcr einen Allergietest zum Nachweis von IgE gegen saisonale Allergene (ELISA basierend auf der Fc-Epsilon-Rezeptor-Technologie) von Juli 2017 bis Juni 2018 eingeschickt wurden. Die Proben wurden in die polypositive Gruppe\u2005A und die nicht polypositive Gruppe\u2005B eingeteilt. Seren der Gruppe\u2005A wurden nach Zugabe eines modifizierten Glykoprotein-Pflanzenextrakts (Blocker) erneut getestet. Um die Auswirkung des Blockers auf jedes Allergen zu bestimmen, wurden die Ergebnisse bei 48 zuf\u00e4llig ausgew\u00e4hlten Proben vor und nach dem Blocken analysiert.<\/jats:p><jats:p>\n               Ergebnisse Die Polysensibilisierung wurde bei 137 (29\u2005%) Serumproben ohne Abh\u00e4ngigkeit von den Jahreszeiten festgestellt. Durch das Blocken wurde die Bindung von Anti-CCD-IgE an Allergenen eliminiert und das Testergebnis entweder negativ (49\u2005%) oder um 1\u20134\u2005Reaktionsklassen erniedrigt (41\u2005%), was die zus\u00e4tzliche Pr\u00e4senz von klinisch relevanten allergenspezifischen IgE anzeigt. Der Anteil an negativen Testreaktionen nach dem Blocken war beim 6-Gr\u00e4ser-Mix (31\u2005%), Roggen (23\u2005%) und Sauerampfer (25\u2005%) viel geringer im Vergleich zu Weide und Birke-Hasel (67\u2005%), Brennnessel und Beifu\u00df-Ragweed (65\u2005%) sowie Spitzwegerich (54\u2005%).<\/jats:p><jats:p>\n               Schlussfolgerung und klinische Relevanz F\u00fcr eine verbesserte Qualit\u00e4t des In-vitro-Allergietests auf IgE gegen saisonale Allergene sollte das Blocken bei multipositiven Ergebnissen angewendet werden, um zu vermeiden, dass bei der allergenspezifischen Immuntherapie (ASIT) klinisch nicht relevante Allergene zum Einsatz kommen.<\/jats:p>","DOI":"10.1055\/a-1274-9188","type":"journal-article","created":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T00:41:44Z","timestamp":1607128904000},"page":"404-409","source":"Crossref","is-referenced-by-count":3,"title":["Pr\u00e4valenz von Immunglobulin E gegen kreuzreagierende Kohlenhydrat-Seitenketten (CCD) und die Wirkung eines Blockers im saisonalen In-vitro-Allergietest"],"prefix":"10.1055","volume":"48","author":[{"given":"Amirhossein","family":"Mohammaddavoodi","sequence":"additional","affiliation":[{"name":"LABOKLIN GmbH & CO. KG, Labor f\u00fcr Klinische Diagnostik"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia","family":"Panakova","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Interne Medizin Kleintiere, Department f\u00fcr Kleintiere und Pferde der Veterin\u00e4rmedizinischen Universit\u00e4t Wien"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Christian","sequence":"additional","affiliation":[{"name":"LABOKLIN GmbH & CO. KG, Labor f\u00fcr Klinische Diagnostik"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iwan","family":"Burgener","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Interne Medizin Kleintiere, Department f\u00fcr Kleintiere und Pferde der Veterin\u00e4rmedizinischen Universit\u00e4t Wien"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"LABOKLIN GmbH & CO. KG, Labor f\u00fcr Klinische Diagnostik"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regina","family":"Wagner","sequence":"additional","affiliation":[{"name":"Dr. R. Wagner GMBH, Raabs\/Thaya, Nieder\u00f6sterreich"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2020,12,4]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1111\/j.1365-3164.1996.tb00246.x","article-title":"An open prospective investigation into aetiology in a group of cats with suspected allergic skin disease","volume":"7","author":"H A O\u2019Dair","year":"1996","journal-title":"Vet Dermatol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1111\/j.1365-3164.2011.00962.x","article-title":"Clinical characteristics and causes of pruritus in cats: a multicentre study on feline hypersensitivity-associated dermatoses","volume":"22","author":"S Hobi","year":"2011","journal-title":"Vet Dermatol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1186\/s12917-015-0515-5","article-title":"Canine atopic dermatitis: detailed guidelines for diagnosis and allergen identification","volume":"11","author":"P Hensel","year":"2015","journal-title":"BMC Vet Res"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/s13601-018-0228-5","article-title":"Atopic dermatitis in cats and dogs: a difficult disease for animals and owners","volume":"8","author":"NK Y Gedon","year":"2018","journal-title":"Clin Transl Allergy"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"25","DOI":"10.3390\/vetsci4020025","article-title":"Cutaneous hypersensitivity dermatoses in the feline patient: a review of allergic skin disease in cats","volume":"4","author":"A Diesel","year":"2017","journal-title":"Vet Sci"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1111\/j.1365-3164.2007.00573.x","article-title":"Pilot study: prevalence of positive aeroallergen reactions in 10 cats with small-airway disease without concurrent skin disease","volume":"18","author":"K A Moriello","year":"2007","journal-title":"Vet Dermatol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1111\/j.1751-0813.1999.tb10263.x","article-title":"Comparison of intradermal testing and serum testing for allergen-specific IgE using monoclonal IgE antibodies in 84 atopic dogs","volume":"77","author":"R S Mueller","year":"1999","journal-title":"Aust Vet J"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0165-2427(03)00052-7","article-title":"Comparison of intradermal and serum testing for allergen-specific IgE using a Fc\u03b5RI\u03b1-based assay in atopic dogs in the UK","volume":"93","author":"A P Foster","year":"2003","journal-title":"Vet Immunol Immunopathol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1024\/0036-7281.150.3.117","article-title":"Comparison between an intradermal skin test and allergen-specific IgE-ELISA for canine atopic dermatitis","volume":"150","author":"Tarpataki, Bigler, Vajdovich","year":"2008","journal-title":"Schweiz Arch Tierheilkd"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1002\/9781118738818.ch36","article-title":"Allergen-specific immunotherapy","author":"A B Diesel","year":"2013","journal-title":"Vet Allergy"},{"key":"ref11","first-page":"629","article-title":"Allergy testing and hyposensitization for 3 common feline dermatoses","volume":"67","author":"B J McDougal","year":"1986","journal-title":"Mod Vet Pract"},{"key":"ref12","first-page":"517","volume-title":"Response to hyposensitization in 29 atopic cats","author":"S Bettenay","year":"1998"},{"key":"ref13","first-page":"25","article-title":"Feline atopic dermatitis: A retrospective study of 45 cases (2001\u20132012)","author":"P A Ravens","year":"2014","journal-title":"Vet Dermatol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1111\/all.13464","article-title":"Allergen immunotherapy in people, dogs, cats and horses \u2013 differences, similarities and research needs","volume":"73","author":"R S Mueller","year":"2018","journal-title":"Allergy"},{"key":"ref15","first-page":"137","article-title":"The effect of serum IgE on an in vitro ELISA test in the normal canine","volume":"1","author":"C E Griffin","year":"1990","journal-title":"Adv Vet Dermatol"},{"key":"ref16","first-page":"545","article-title":"Evaluation of the performance of a serologic allergy system in atopic dogs. 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