{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T22:39:38Z","timestamp":1783723178968,"version":"3.55.0"},"reference-count":27,"publisher":"Georg Thieme Verlag KG","issue":"03","funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"crossref","award":["2015\/08600-2"],"award-info":[{"award-number":["2015\/08600-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"crossref","award":["2016\/23816-4"],"award-info":[{"award-number":["2016\/23816-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Pterodon pubescens fruits are popularly used because of their analgesic and anti-inflammatory actions, which are attributed to the isolated compounds with a vouacapan skeleton. This work aimed to evaluate the antiproliferative and anti-inflammatory effects of a P. pubescens fruit dichloromethane extract and the vouacapan diterpene furan isomer\u02bcs mixture (1\u200a:\u200a1) (6\u03b1-hydroxy-7\u03b2-acetoxy-vouacapan-17\u03b2-oate methyl ester and 6\u03b1-acetoxy-7\u03b2-hydroxy-vouacapan-17\u03b2-oate methyl ester isomers) in HaCaT cells using the cell migration and the BrDU incorporation assay. Levels of IL-8 were measured by ELISA after TNF-\u03b1 stimulation. HPLC\/DAD analysis of the extract revealed the expressive presence of vouacapan diterpene furan isomer\u02bcs mixture. P. pubescens extract (1.5625\u200a\u2013\u200a25\u2009\u00b5g\/mL) and vouacapan diterpene furan isomer\u02bcs mixture (3.125\u200a\u2013\u200a50\u2009\u00b5M) inhibited cell proliferation as indicated by a decreased\n\t\t\t\t\tBrdU-incorporation. For the evaluation of cell migration, time-lapse microscopy was used. P. pubescens presented inhibition on cell migration at all concentrations tested (3.125\u200a\u2013\u200a12.5\u2009\u00b5g\/mL), whereas for the VDFI mixture, the inhibition was only observed at the highest concentrations (12.5 and 25\u2009\u00b5M) tested. Furthermore P. pubescens extract and vouacapan diterpene furan isomer\u02bcs mixture significantly decreased IL-8 levels. Our results showed antiproliferative and anti-inflammatory effects on HaCaT cells treated with the extract and the vouacapan isomer\u02bcs mixture, without affecting cell viability. These activities could be attributed to the voucapan molecular structures. In conclusion, topical products developed of P. pubescens extract or the voucapan isomer\u02bcs mixture should be further studied as a potential product for local treatment against hyperproliferative lesions as in psoriasis vulgaris, representing an alternative treatment approach.<\/jats:p>","DOI":"10.1055\/a-1279-0645","type":"journal-article","created":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T23:44:31Z","timestamp":1604447071000},"page":"218-224","source":"Crossref","is-referenced-by-count":4,"title":["Antiproliferative Effects of Pterodon pubescens Extract and Isolated Diterpenes in HaCaT Cells"],"prefix":"10.1055","volume":"87","author":[{"given":"Rosanna Tarkany","family":"Basting","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences (FCF), State University of Campinas (Unicamp), Campinas, SP, Brazil"},{"name":"Postgraduate Program in Medical Sciences, Faculty of Medical Science (FCM), University of Campinas (Unicamp), Campinas, SP, Brazil"},{"name":"Chemical, Biological and Agricultural Research Center (CPQBA), State University of Campinas (Unicamp), Paul\u00ednia, SP, Brazil"},{"name":"S\u00e3o Leopoldo Mandic Institute and Research Center, Laboratory of Neuroimmune Interface of Pain Research, Campinas, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilza Maria","family":"de Oliveira Sousa","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences (FCF), State University of Campinas (Unicamp), Campinas, SP, Brazil"},{"name":"Postgraduate Program in Medical Sciences, Faculty of Medical Science (FCM), University of Campinas (Unicamp), Campinas, SP, Brazil"},{"name":"Chemical, Biological and Agricultural Research Center (CPQBA), State University of Campinas (Unicamp), Paul\u00ednia, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"Butterweck","sequence":"additional","affiliation":[{"name":"Institute for Pharma Technology, School of Life Sciences, University of Applied Sciences Northwestern Switzerland-FHNW, Muttenz, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary Ann","family":"Foglio","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences (FCF), State University of Campinas (Unicamp), Campinas, SP, Brazil"},{"name":"Chemical, Biological and Agricultural Research Center (CPQBA), State University of Campinas (Unicamp), Paul\u00ednia, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2020,11,3]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.phrs.2016.01.021","article-title":"Medicinal plants in Brazil: pharmacological studies, drug discovery, challenges and perspectives","volume":"112","author":"R C Dutra","year":"2016","journal-title":"Pharmacol Res"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.ejphar.2017.03.030","article-title":"Derivatives of furanditerpenes from Pterodon genus: pharmacological studies disclose their potential as chronic pain relief in mice","volume":"804","author":"H M Spindola","year":"2017","journal-title":"Eur J of Pharmacol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.jep.2015.09.005","article-title":"Ameliorative potential of standardized fruit extract of Pterodon pubescens Benth on neuropathic pain in mice: Evidence for the mechanism of action","volume":"175","author":"C Nucci-Martins","year":"2015","journal-title":"J Ethnopharmacol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1590\/S0103-50532009000300024","article-title":"Furanoditerpenes from Pterodon pubescens Benth. with selective in vitro anticancer activity for prostate cell line","volume":"20","author":"H M Spindola","year":"2009","journal-title":"J Brazil Chem Soc"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1590\/S0103-50532012000700008","article-title":"Pterodon pubescens Benth. 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