{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T20:37:17Z","timestamp":1771187837847,"version":"3.50.1"},"reference-count":25,"publisher":"Georg Thieme Verlag KG","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Exp Clin Endocrinol Diabetes"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Polycystic ovary syndrome (PCOS) in an intricate disorder characterized by reproductive and metabolic abnormalities that may affect bone quality and strength along with the lifespan. The present study analysed the impact of postnatal androgenization (of a single dose of testosterone propionate 1.25\u2009mg subcutaneously at day 5 of life) on bone development and markers of bone metabolism in adult female Wistar rats. Compared with healthy controls, the results of measurements of micro-computed tomography (microCT) of the distal femur of androgenized rats indicated an increased cortical bone volume voxel bone volume to total volume (VOX BV\/TV) and higher trabecular number (Tb.n) with reduced trabecular separation (Tb.sp). A large magnitude effect size was observed in the levels of circulating bone formation Procollagen I N-terminal propeptide (P1NP) at day 60 of life; reabsorption cross-linked C-telopeptide of type I collagen (CTX) markers were similar between the androgenized and control rats at days 60 and 110 of life. The analysis of gene expression in bone indicated elements for an increased bone mass such as the reduction of the Dickkopf-1 factor (Dkk1) a negative regulator of osteoblast differentiation (bone formation) and the reduction of Interleukin 1-b (Il1b), an activator of osteoclast differentiation (bone reabsorption). Results from this study highlight the possible role of the developmental programming on bone microarchitecture with reference to young women with PCOS.<\/jats:p>","DOI":"10.1055\/a-1284-5491","type":"journal-article","created":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T23:43:41Z","timestamp":1603928621000},"page":"757-761","source":"Crossref","is-referenced-by-count":4,"title":["Trabecular Bone is Increased in a Rat Model of Polycystic Ovary Syndrome"],"prefix":"10.1055","volume":"129","author":[{"given":"Lady Katerine Serrano","family":"Mujica","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Werner Giehl","family":"Glanzner","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda Luiza","family":"Prante","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vitor Braga","family":"Rissi","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabrielle Rebeca Everling","family":"Correa","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melissa Orlandin","family":"Premaor","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, Federal University of Santa Maria (UFSM), Santa Maria, RS"},{"name":"MOP and FVC are now at the Department of Clinical Medicine, Federal University of Minas Gerais,  Belo Horizonte, MG"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfredo Quites","family":"Antoniazzi","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Bayard Dias","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabio Vasconcellos","family":"Comim","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, 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Med"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s00223-008-9126-5","article-title":"Determinants of bone turnover markers in healthy premenopausal women","volume":"82","author":"S Adami","year":"2008","journal-title":"Calcif Tissue Int"},{"key":"ref4","first-page":"2821","article-title":"Augmentation of bone mineral density in hirsute women","volume":"82","author":"S DagogoJack","year":"1997","journal-title":"J Clin Endocr Metab"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"3496","DOI":"10.1210\/jcem.85.10.6902","article-title":"Bone mineral density, androgens, and the polycystic ovary: The complex and controversial issue of androgenic influence in female bone","volume":"85","author":"J V Zborowski","year":"2000","journal-title":"J Clin Endocr Metab"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.maturitas.2017.12.010","article-title":"Causes of low peak bone mass in women","volume":"111","author":"C K Chew","year":"2018","journal-title":"Maturitas"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"2107","DOI":"10.1093\/humrep\/deq149","article-title":"Augmentation of cortical bone mineral density in women with polycystic ovary syndrome: A peripheral quantitative computed tomography (pQCT) study","volume":"25","author":"D Kassanos","year":"2010","journal-title":"Human Reproduction"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1093\/humupd\/dmz020","article-title":"Negative impact of polycystic ovary syndrome on bone health: A systematic review and meta-analysis","volume":"25","author":"J M Piovezan","year":"2019","journal-title":"Human reproduction update"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1016\/j.fertnstert.2005.06.017","article-title":"Effects of testosterone on cancellous bone, marrow adipocytes, and ovarian phenotype in a young female rat model of polycystic ovary syndrome","volume":"84","author":"N Tamura","year":"2005","journal-title":"Fertility and Sterility"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.mce.2016.12.015","article-title":"The impact of postnatal leuprolide acetate treatment on reproductive characteristics in a rodent model of polycystic ovary syndrome","volume":"442","author":"L K Serrano Mujica","year":"2017","journal-title":"Mol Cell Endocrinol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"193","DOI":"10.2147\/JIR.S160264","article-title":"Oxidative stress and metabolic markers in pre- and postnatal polycystic ovary syndrome rat protocols","volume":"11","author":"L Serrano Mujica","year":"2018","journal-title":"J Inflamm Res"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"2678","DOI":"10.1210\/en.2015-1235","article-title":"Developmental programming: Prenatal and postnatal androgen antagonist and insulin sensitizer interventions prevent advancement of puberty and improve lh surge dynamics in prenatal testosterone-treated sheep","volume":"156","author":"V Padmanabhan","year":"2015","journal-title":"Endocrinology"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1037\/0033-2909.112.1.155","article-title":"A power primer","volume":"112","author":"J Cohen","year":"1992","journal-title":"Psychol Bull"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1038\/d41586-019-00857-9","article-title":"Scientists rise up against statistical significance","volume":"567","author":"V Amrhein","year":"2019","journal-title":"Nature"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"3493","DOI":"10.1210\/jcem.82.10.4319","article-title":"The localization of androgen receptors in human bone","volume":"82","author":"E O Abu","year":"1997","journal-title":"J Clin Endocr Metab"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1210\/endo-68-1-62","article-title":"Production of anovulatory, sterile rats by single injections of testosterone propionate","volume":"68","author":"C Barraclough","year":"1961","journal-title":"Endocrinology"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/S8756-3282(99)00018-6","article-title":"Effects of growth hormone and testosterone on cortical bone formation and bone density in aged orchiectomized rats","volume":"24","author":"G Prakasam","year":"1999","journal-title":"Bone"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1002\/jor.1100080418","article-title":"Differential effects of androgens on cortical bone histomorphometry in gonadectomized male and female rats","volume":"8","author":"R T Turner","year":"1990","journal-title":"J Orthop Res"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"E540","DOI":"10.1152\/ajpendo.00161.2017","article-title":"Cross-sex testosterone therapy in ovariectomized mice: addition of low-dose estrogen preserves bone architecture","volume":"313","author":"L G Goetz","year":"2017","journal-title":"Am J Physiol Endocrinol Metab"},{"key":"ref20","first-page":"146","article-title":"Estrogen-progestin therapy causes a greater increase in spinal bone mineral density than estrogen therapy - a systematic review and meta-analysis of controlled trials with direct randomization","volume":"17","author":"J C Prior","year":"2017","journal-title":"J Musculoskelet Neuronal Interact"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.jpag.2011.11.010","article-title":"Effect of antiandrogen treatment on bone density and bone geometry in adolescents with polycystic ovary syndrome","volume":"25","author":"S Bechtold","year":"2012","journal-title":"J Pediatr Adolesc Gynecol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1007\/s00774-010-0211-2","article-title":"Serum concentrations of carboxylated osteocalcin are increased and associated with several components of the polycystic ovarian syndrome","volume":"29","author":"E Diamanti-Kandarakis","year":"2011","journal-title":"Journal of Bone and Mineral Metabolism"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1111\/cen.13456","article-title":"Bone markers in polycystic ovary syndrome: A multicentre study","volume":"87","author":"S Lingaiah","year":"2017","journal-title":"Clinical Endocrinology"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1046\/j.1365-2265.1998.3691182.x","article-title":"Effect of hyperandrogenism and menstrual cycle abnormalities on bone mass and bone turnover in young women","volume":"48","author":"S Adami","year":"1998","journal-title":"Clin Endocrinol (Oxf)"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1007\/s00774-013-0560-8","article-title":"Association of plasma GDF-9 or GDF-15 levels with bone parameters in polycystic ovary syndrome","volume":"33","author":"Z Berberoglu","year":"2015","journal-title":"Journal 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