{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:34:13Z","timestamp":1740126853746,"version":"3.37.3"},"reference-count":26,"publisher":"Georg Thieme Verlag KG","issue":"08","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Klin Monbl Augenheilkd"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Aim This retrospective investigated the impact of donor age, recipient age, donor endothelial cell density, vis-\u00e0-tergo, and additional intraoperative lens exchange (triple-procedure) on overall early and late phase postoperative endothelial cell density (ECD) following penetrating keratoplasty (PKP) in various diagnosis groups.<\/jats:p><jats:p>\n          Patients and Methods In 590 cases with diagnosed keratoconus (KC), Fuchs dystrophy (FD) and herpes simplex virus infection (HSV) who underwent PKP or triple surgery, the ECD in cells\/mm2 was analysed, both preoperatively, with all-sutures-in (early postoperative stage), and after last suture removal. The factors were tested by Mann-Whitney U-test, correlation analysis and linear regression analysis.<\/jats:p><jats:p>\n          Outcome Correlation analysis demonstrated a weak negative correlation between the patient\u02bcs ECD and donor age (early postoperative stage: r\u2009=\u2009\u2212\u20090.25, p\u2009&lt;\u20090.001; after last suture removal: r\u2009=\u2009\u2212\u20090.16; p\u2009=\u20090.003). Regression analysis revealed that donor age did not impact postoperative patient ECD. There was a weak negative correlation between postoperative ECD and recipient age (early postoperative stage: r\u2009=\u2009\u2212\u20090.31, p\u2009&lt;\u20090.001; after last suture removal: r\u2009=\u2009\u2212\u20090.34, p\u2009&lt;\u20090.001). Regression analysis confirmed the negative impact of recipient age on patient ECD (early postoperative stage: \u03b2\u2009=\u2009\u2212\u200913.2, p\u2009=\u20090.001; after last suture removal: \u03b2\u2009=\u2009\u2212\u20094.6, p\u2009&lt;\u20090.001). Correlation analysis determined a weak positive correlation between postoperative ECD and donor endothelial cell density (early postoperative stage: r\u2009=\u20090.37, p\u2009&lt;\u20090.001; after last suture removal: r\u2009=\u20090.32, p\u2009&lt;\u20090.001). Regression analysis also determined that donor endothelial cell density had\n\t\t\t\t\ta positive impact on postoperative ECD following last suture removal (\u03b2\u2009=\u20090.4, p\u2009&lt;\u20090.001). Vis-\u00e0-tergo and additional lens exchange (triple procedure) had no significant effect on postoperative ECD (p\u2009&gt;\u20090.05). This was also confirmed by the results of the regression analysis after last suture removal.<\/jats:p><jats:p>\n          Conclusion Recipient age and donor endothelial cell density have a significant impact on postoperative ECD following PKP. Not all of the statistical tests proved donor age to be a significant influencing factor. Vis-\u00e0-tergo and additional lens exchange (triple procedure) had no significant effect on postoperative ECD following PKP.<\/jats:p>","DOI":"10.1055\/a-1333-2723","type":"journal-article","created":{"date-parts":[[2021,5,17]],"date-time":"2021-05-17T23:01:12Z","timestamp":1621292472000},"page":"904-911","source":"Crossref","is-referenced-by-count":2,"title":["Impact of Pre- and Intraoperative Factors on Endothelial Cell Density in the Early and Late Stage after Penetrating Keratoplasty"],"prefix":"10.1055","volume":"238","author":[{"given":"Jessica","family":"Brand","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Augenheilkunde, Universit\u00e4tsklinikum des Saarlandes, Homburg\/Saar, Deutschland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Achim","family":"Langenbucher","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Experimentelle Ophthalmologie, Universit\u00e4t des Saarlandes, Homburg\/Saar, Deutschland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Zemova","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Augenheilkunde, Universit\u00e4tsklinikum des Saarlandes, Homburg\/Saar, Deutschland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanja","family":"Stachon","sequence":"additional","affiliation":[{"name":"Dr. Rolf M. Schwiete Zentrum f\u00fcr Limbusstammzellforschung und kongenitale Aniridie, Universit\u00e4t des Saarlandes, Homburg\/Saar, Deutschland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melanie","family":"Weber","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Augenheilkunde, Universit\u00e4tsklinikum des Saarlandes, Homburg\/Saar, Deutschland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerome","family":"Rebmann","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Augenheilkunde, Universit\u00e4tsklinikum des Saarlandes, Homburg\/Saar, Deutschland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9701-8204","authenticated-orcid":false,"given":"Berthold","family":"Seitz","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Augenheilkunde, Universit\u00e4tsklinikum des Saarlandes, Homburg\/Saar, Deutschland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N\u00f3ra","family":"Szentm\u00e1ry","sequence":"additional","affiliation":[{"name":"Dr. Rolf M. Schwiete Zentrum f\u00fcr Limbusstammzellforschung und kongenitale Aniridie, Universit\u00e4t des Saarlandes, Homburg\/Saar, Deutschland"},{"name":"Klinik f\u00fcr Augenheilkunde, Semmelweis Universit\u00e4t, Budapest, Ungarn"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2021,5,17]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1055\/s-0031-1273254","article-title":"Physiologie des humanen Hornhautendothels neue Erkenntnisse durch elektrophysiologische Untersuchungen","volume":"228","author":"S Mergler","year":"2011","journal-title":"Klin Monbl Augenheilkd"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"671","DOI":"10.3109\/02713688509017661","article-title":"Changes in the normal corneal endothelial cellular pattern as a function of age","volume":"4","author":"R W Yee","year":"1985","journal-title":"Curr Eye Res"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1055\/s-0035-1545816","article-title":"Ist die Abnahme der Hornhaut-Endothelzellzahl mit dem Alter wirklich linear?","volume":"232","author":"R Beuschel","year":"2015","journal-title":"Klin Monbl Augenheilkd"},{"key":"ref4","first-page":"403","article-title":"Morphologic and functional evaluation of the endothelium of transplanted human corneas","volume":"81","author":"W Bourne","year":"1983","journal-title":"Trans Am Ophthalmol Soc"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1016\/S0002-9394(14)78102-4","article-title":"Endothelial cell loss during penetrating keratoplasty","volume":"85","author":"W Bourne","year":"1978","journal-title":"Am J Ophthalmol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1055\/s-2008-1054967","article-title":"Morphologische Kriterien der Transplantatauswahl und Verlaufskontrollen bei perforierender Keratoplastik","volume":"183","author":"R Winter","year":"1983","journal-title":"Klin Monbl Augenheilkd"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"1184","DOI":"10.1055\/s-0035-1545734","article-title":"Einfluss der individuellen mikrochirurgischen Technik auf Endothelzelldichte und Pachymetrie nach perforierender Keratoplastik bei Patienten mit Fuchs-Dystrophie oder Keratokonus","volume":"232","author":"N Szentm\u00e1ry","year":"2015","journal-title":"Klin Monbl Augenheilkd"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"975","DOI":"10.1007\/s00417-003-0701-1","article-title":"Predictive donor factors for chronic endothelial cell loss after nonmechanical penetrating keratoplasty in a regression model","volume":"241","author":"A Langenbucher","year":"2003","journal-title":"Graefes Arch Clin Exp Ophthalmol"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"986","DOI":"10.1136\/bjophthalmol-2015-306871","article-title":"The impact of donor age and endothelial cell density on graft survival following penetrating keratoplasty","volume":"100","author":"M J Wakefield","year":"2016","journal-title":"Br J Ophthalmol"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"2428","DOI":"10.1016\/j.ophtha.2013.08.044","article-title":"Donor age and factors related to endothelial cell loss 10 years after penetrating keratoplasty: Specular microscopy ancillary study","volume":"120","author":"J H Lass","year":"2013","journal-title":"Ophthalmology"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1007\/s003470170117","article-title":"Verlauf der Endothelzelldichte nach perforierender Keratoplastik. 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Culbertson","year":"1982","journal-title":"Ophthalmology"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1007\/s004170000225","article-title":"Graft endothelium and thickness after penetrating keratoplasty, comparing mechanical and excimer laser trephination: A prospective randomised study","volume":"239","author":"B Seitz","year":"2001","journal-title":"Graefes Arch Clin Exp Ophthalmol"},{"key":"ref25","first-page":"28","article-title":"[Corneal endothelial cell changes in triple procedure]","volume":"106","author":"E Suzuki","year":"2002","journal-title":"Nihon Ganka Gakkai Zasshi"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"1093","DOI":"10.1007\/s00347-004-1031-3","article-title":"Hinterkammerlinsenimplantation nach perforierender Keratoplastik: Mitursache f\u00fcr das sp\u00e4te Transplantatversagen?","volume":"101","author":"D B\u00f6hringer","year":"2004","journal-title":"Ophthalmologe"}],"container-title":["Klinische Monatsbl\u00e4tter f\u00fcr Augenheilkunde"],"original-title":[],"language":"de","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-1333-2723.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,19]],"date-time":"2021-08-19T22:36:45Z","timestamp":1629412605000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-1333-2723"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,17]]},"references-count":26,"journal-issue":{"issue":"08","published-online":{"date-parts":[[2021,8,19]]},"published-print":{"date-parts":[[2021,8]]}},"URL":"https:\/\/doi.org\/10.1055\/a-1333-2723","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["0023-2165","1439-3999"],"issn-type":[{"type":"print","value":"0023-2165"},{"type":"electronic","value":"1439-3999"}],"subject":[],"published":{"date-parts":[[2021,5,17]]}}}