{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:47:38Z","timestamp":1787878058852,"version":"build-2784847793"},"reference-count":28,"publisher":"Georg Thieme Verlag KG","issue":"05","license":[{"start":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T00:00:00Z","timestamp":1619395200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Deutsche Forschungsgemeinschaft (DFG) CRC\/Transregio 205\/1","award":["314061271"],"award-info":[{"award-number":["314061271"]}]},{"name":"Deutsche Forschungsgemeinschaft (DFG) CRC\/Transregio 205\/1","award":["515\/28\u20131"],"award-info":[{"award-number":["515\/28\u20131"]}]},{"name":"German Ministry\n                        of Education and Research and the Brandenburg","award":["82DZD00302"],"award-info":[{"award-number":["82DZD00302"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Horm Metab Res"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The adrenal gland is important for many physiological and pathophysiological\n                    processes, but studies are often restricted by limited availability of sample\n                    material. Improved methods for sample preparation are needed to facilitate\n                    analyses of multiple classes of adrenal metabolites and macromolecules in a\n                    single sample. A procedure was developed for preparation of chromaffin cells,\n                    mouse adrenals, and human chromaffin tumors that allows for multi-omics analyses\n                    of different metabolites and preservation of native proteins. To evaluate the\n                    new procedure, aliquots of samples were also prepared using conventional\n                    procedures. Metabolites were analyzed by liquid-chromatography with mass\n                    spectrometry or electrochemical detection. Metabolite contents of chromaffin\n                    cells and tissues analyzed with the new procedure were similar or even higher\n                    than with conventional methods. Catecholamine contents were comparable between\n                    both procedures. The TCA cycle metabolites, cis-aconitate, isocitate, and\n                    \u03b1-ketoglutarate were detected at higher concentrations in cells, while\n                    in tumor tissue only isocitrate and potentially fumarate were measured at higher\n                    contents. In contrast, in a broad untargeted metabolomics approach, a\n                    methanol-based preparation procedure of adrenals led to a 1.3-fold higher number\n                    of detected metabolites. The established procedure also allows for simultaneous\n                    investigation of adrenal hormones and related enzyme activities as well as\n                    proteins within a single sample. This novel multi-omics approach not only\n                    minimizes the amount of sample required and overcomes problems associated with\n                    tissue heterogeneity, but also provides a more complete picture of adrenal\n                    function and intra-adrenal interactions than previously possible.<\/jats:p>","DOI":"10.1055\/a-1440-0278","type":"journal-article","created":{"date-parts":[[2021,4,27]],"date-time":"2021-04-27T00:09:23Z","timestamp":1619482163000},"page":"326-334","source":"Crossref","is-referenced-by-count":19,"title":["Adrenal Hormone Interactions and Metabolism: A Single Sample\n                    Multi-Omics Approach"],"prefix":"10.1055","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6932-333X","authenticated-orcid":false,"given":"Nicole","family":"Bechmann","sequence":"additional","affiliation":[{"name":"Institute of Clinical Chemistry and Laboratory Medicine, Technische\n                    Universit\u00e4t Dresden, Dresden, Germany"},{"name":"Department of Medicine III, Technische Universit\u00e4t Dresden,\n                    Dresden, Germany"},{"name":"German Institute of Human Nutrition Potsdam-Rehbruecke, Department of\n                    Experimental Diabetology, Nuthetal, Germany"},{"name":"German Center for Diabetes Research (DZD), M\u00fcnchen-Neuherberg,\n                    Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepika","family":"Watts","sequence":"additional","affiliation":[{"name":"Institute of Clinical Chemistry and Laboratory Medicine, Technische\n                    Universit\u00e4t Dresden, Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9635-4860","authenticated-orcid":false,"given":"Charlotte","family":"Steenblock","sequence":"additional","affiliation":[{"name":"Department of Medicine III, Technische Universit\u00e4t Dresden,\n                    Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paal William","family":"Wallace","sequence":"additional","affiliation":[{"name":"Institute of Clinical Chemistry and Laboratory Medicine, Technische\n                    Universit\u00e4t Dresden, Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette","family":"Sch\u00fcrmann","sequence":"additional","affiliation":[{"name":"German Institute of Human Nutrition Potsdam-Rehbruecke, Department of\n                    Experimental Diabetology, Nuthetal, Germany"},{"name":"German Center for Diabetes Research (DZD), M\u00fcnchen-Neuherberg,\n                    Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan R.","family":"Bornstein","sequence":"additional","affiliation":[{"name":"Department of Medicine III, Technische Universit\u00e4t Dresden,\n                    Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Wielockx","sequence":"additional","affiliation":[{"name":"Institute of Clinical Chemistry and Laboratory Medicine, Technische\n                    Universit\u00e4t Dresden, Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graeme","family":"Eisenhofer","sequence":"additional","affiliation":[{"name":"Institute of Clinical Chemistry and Laboratory Medicine, Technische\n                    Universit\u00e4t Dresden, Dresden, Germany"},{"name":"Department of Medicine III, Technische Universit\u00e4t Dresden,\n                    Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirko","family":"Peitzsch","sequence":"additional","affiliation":[{"name":"Institute of Clinical Chemistry and Laboratory Medicine, Technische\n                    Universit\u00e4t Dresden, Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2021,4,26]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1007\/s10571-010-9606-9","article-title":"Adrenal responses to stress","volume":"30","author":"D S Goldstein","year":"2010","journal-title":"Cell Mol Neurobiol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.jsbmb.2019.04.006","article-title":"The adrenal gland in stress\u2013Adaptation on a cellular level","volume":"190","author":"I Berger","year":"2019","journal-title":"J Steroid Biochem Mol Biol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1210\/er.2010-0013","article-title":"The molecular biology, biochemistry, and physiology of human steroidogenesis and\n                    its disorders","volume":"32","author":"W L Miller","year":"2011","journal-title":"Endocr Rev"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.coemr.2019.06.005","article-title":"Adrenal cortex\u2013medulla interactions in adaptation to stress and\n                    disease","volume":"8","author":"S R Bornstein","year":"2019","journal-title":"Curr Opin Endocr Metab Res"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1007\/s00216-012-6599-x","article-title":"Double stable isotope ultra performance liquid chromatographic-tandem mass\n                    spectrometric quantification of tissue content and activity of\n                    phenylethanolamine N-methyltransferase, the crucial enzyme responsible for\n                    synthesis of epinephrine","volume":"405","author":"N Qin","year":"2013","journal-title":"Anal Bioanal Chem"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1464","DOI":"10.1126\/science.150.3702.1464","article-title":"Adrenaline synthesis: control by the pituitary gland and 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Endocrinol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"e3374","DOI":"10.1210\/clinem\/dgaa423","article-title":"Glucocorticoid excess in patients with pheochromocytoma compared with\n                    paraganglioma and other forms of hypertension","volume":"105","author":"G Constantinescu","year":"2020","journal-title":"J Clin Endocrinol Metab"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1206","DOI":"10.1056\/NEJM199610173351607","article-title":"Adrenal insufficiency","volume":"335","author":"W Oelkers","year":"1996","journal-title":"N Engl J Med"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1373\/clinchem.2004.045484","article-title":"Pheochromocytoma catecholamine phenotypes and prediction of tumor size and\n                    location by use of plasma free metanephrines","volume":"51","author":"G Eisenhofer","year":"2005","journal-title":"Clin 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responses to\n                    inhibition of monoamine oxidase","volume":"32","author":"G Eisenhofer","year":"1986","journal-title":"Clin Chem"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"594","DOI":"10.3390\/cancers11050594","article-title":"Impact of extrinsic and intrinsic hypoxia on catecholamine biosynthesis in\n                    absence or presence of HIF2\u03b1 in pheochromocytoma cells","volume":"11","author":"N Bechmann","year":"2019","journal-title":"Cancers"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.jsbmb.2014.10.006","article-title":"An LC\u2013MS\/MS method for steroid profiling during adrenal venous\n                    sampling for investigation of primary aldosteronism","volume":"145","author":"M Peitzsch","year":"2015","journal-title":"J Steroid Biochem Mol Biol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1038\/s41436-018-0106-5","article-title":"Metabolome-guided genomics to identify pathogenic variants in isocitrate\n                    dehydrogenase, fumarate hydratase, and succinate dehydrogenase genes in\n                    pheochromocytoma and paraganglioma","volume":"21","author":"S Richter","year":"2019","journal-title":"Genet Med"},{"key":"ref19","first-page":"D608","article-title":"HMDB 4.0: the human metabolome database for 2018","volume":"46(D1)","author":"D S Wishart","year":"2017","journal-title":"Nucleic Acids Res"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: kyoto encyclopedia of genes and genomes","volume":"28","author":"M Kanehisa","year":"2000","journal-title":"Nucleic Acids Res"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"172","DOI":"10.3390\/md16050172","article-title":"Anti-tumorigenic and anti-metastatic activity of the sponge-derived marine drugs\n                    aeroplysinin-1 and isofistularin-3 against pheochromocytoma in vitro","volume":"16","author":"N Bechmann","year":"2018","journal-title":"Mar Drugs"},{"key":"ref22","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1016\/j.ccell.2017.01.001","article-title":"Comprehensive molecular characterization of\n                    pheochromocytoma and paraganglioma","volume":"31","author":"L Fishbein","year":"2017","journal-title":"Cancer\n                    Cell"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"3685","DOI":"10.1172\/JCI69741","article-title":"Targeting lactate metabolism for cancer therapeutics","volume":"123","author":"J R Doherty","year":"2013","journal-title":"J Clin Invest"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1016\/j.ejca.2011.07.016","article-title":"Plasma methoxytyramine: a novel biomarker of metastatic pheochromocytoma and\n                    paraganglioma in relation to established risk factors of tumour size, location\n                    and SDHB mutation status","volume":"48","author":"G Eisenhofer","year":"2012","journal-title":"Eur J Cancer"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1530\/ERC-20-0205","article-title":"HIF2\u03b1 supports pro-metastatic behavior in\n                    pheochromocytomas\/paragangliomas","volume":"27","author":"N Bechmann","year":"2020","journal-title":"Endocr Relat Cancer"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1677\/ERC-10-0211","article-title":"Catecholamine metabolomic and secretory phenotypes in phaeochromocytoma","volume":"18","author":"G Eisenhofer","year":"2011","journal-title":"Endocr Relat Cancer"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"5279","DOI":"10.1007\/s00216-013-6927-9","article-title":"Optimization of harvesting, extraction, and analytical protocols for\n                    UPLC-ESI-MS-based metabolomic analysis of adherent mammalian cancer cells","volume":"405","author":"H Bi","year":"2013","journal-title":"Anal Bioanal Chem"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.ab.2010.04.031","article-title":"Towards quantitative metabolomics of mammalian cells: development of a\n                    metabolite extraction protocol","volume":"404","author":"S Dietmair","year":"2010","journal-title":"Anal Biochem"}],"container-title":["Hormone and Metabolic Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-1440-0278.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,7]],"date-time":"2021-05-07T23:04:43Z","timestamp":1620428683000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-1440-0278"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,26]]},"references-count":28,"journal-issue":{"issue":"05","published-online":{"date-parts":[[2021,5,7]]},"published-print":{"date-parts":[[2021,5]]}},"URL":"https:\/\/doi.org\/10.1055\/a-1440-0278","relation":{},"ISSN":["0018-5043","1439-4286"],"issn-type":[{"value":"0018-5043","type":"print"},{"value":"1439-4286","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,26]]}}}