{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T07:49:44Z","timestamp":1767340184151,"version":"3.37.3"},"reference-count":36,"publisher":"Georg Thieme Verlag KG","issue":"06","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Klin Monbl Augenheilkd"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Background Cornea guttata may not be recognized in the eye bank and recent studies have displayed that guttae are transplanted in about 15% of cases in varying severities. The purpose of this study was to establish semiquantitative criteria for the detection of cornea guttata in donor corneas in the eye bank.<\/jats:p><jats:p>Methods In this retrospective cohort study, preoperative endothelial pictures of donor corneas were collected and classified according to the post-penetrating keratoplasty cornea guttata grade into three distinct groups: group 1 consists of healthy corneas with no guttae (guttata grade 0); group 2 constitutes corneas with mild asymptomatic cornea guttata (guttata grade +); and group 3 comprises corneas with advanced widespread cornea guttata (guttata grade ++\/+++\/++++). The preoperative pictures of each group were then individually analyzed using the following five semiquantitative criteria: The number and the area of the cell-depleted surfaces, the presence of less than 50% of the cells having a hexagonal or a circular shape, the presence of cell membrane defects and interruptions, the presence of blebs in the cell membrane, and the presence of groups of cells with a distinct whitish color.<\/jats:p><jats:p>Results In total, 262 patients were included in this study, with a total number of 1582 preoperative donor corneal endothelial pictures. Out of those pictures, groups 1, 2, and 3 encompassed 995 (62.9%), 411 (26.0%), and 176 (11.1%) pictures, respectively. Three out of the five eye bank criteria were found to correlate with postoperative cornea guttata with a highly significant p value of &lt;\u20090.001. These three criteria are the presence of less than 50% of the cells having a hexagonal or a circular shape, the presence of cell membrane defects and interruptions and, the presence of blebs. The presence of groups of cells with a distinct whitish color was only a weak predictive factor for cornea guttata (p\u2009=\u20090.069). There was no statistically significant correlation between the number and the area of cell-depleted surfaces and postoperative cornea guttata with a p\u2009=\u20090.181.<\/jats:p><jats:p>Conclusion Three semiquantitative criteria that can be detected in the eye bank using inverted light microscopy seem to correlate with postoperative cornea guttata: The presence of blebs, the presence of cell membrane defects and interruptions, as well as endothelial pictures with less than 50% of the cells having a hexagonal of circular shape. The presence of groups of cells with a distinct whitish color appears to be a weak predictor of cornea guttata.<\/jats:p>","DOI":"10.1055\/a-1498-1846","type":"journal-article","created":{"date-parts":[[2021,6,22]],"date-time":"2021-06-22T22:35:38Z","timestamp":1624401338000},"page":"680-687","source":"Crossref","is-referenced-by-count":14,"title":["Semiquantitative Criteria in the Eye Bank That Correlate with Cornea Guttata in Donor Corneas"],"prefix":"10.1055","volume":"238","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1073-4185","authenticated-orcid":false,"given":"Tarek","family":"Safi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Saarland University Hospital and Saarland University Faculty of Medicine, Homburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Loay","family":"Daas","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Saarland University Hospital and Saarland University Faculty of Medicine, Homburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gian-Luca","family":"Kiefer","sequence":"additional","affiliation":[{"name":"Department of Cognitive Assistants, German Research Centre for Artificial Intelligence Saarbrucken Branch, Saarbrucken, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mansi","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Cognitive Assistants, German Research Centre for Artificial Intelligence Saarbrucken Branch, Saarbrucken, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alassane","family":"Ndiaye","sequence":"additional","affiliation":[{"name":"Department of Cognitive Assistants, German Research Centre for Artificial Intelligence Saarbrucken Branch, Saarbrucken, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthieu","family":"Deru","sequence":"additional","affiliation":[{"name":"Department of Cognitive Assistants, German Research Centre for Artificial Intelligence Saarbrucken Branch, Saarbrucken, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Alexandersson","sequence":"additional","affiliation":[{"name":"Department of Cognitive Assistants, German Research Centre for Artificial Intelligence Saarbrucken Branch, Saarbrucken, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9701-8204","authenticated-orcid":false,"given":"Berthold","family":"Seitz","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Saarland University Hospital and Saarland University Faculty of Medicine, Homburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2021,6,22]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/j.ajo.2018.01.018","article-title":"Trends in Corneal Transplantation from 2001 to 2016 in Germany: A Report of the DOG-Section Cornea and its Keratoplasty Registry","volume":"188","author":"E Flockerzi","year":"2018","journal-title":"Am J Ophthalmol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"457","DOI":"10.4103\/0301-4738.67064","article-title":"Advances in keratoplasty procedures: A review","volume":"58","author":"S Rajesh","year":"2010","journal-title":"Indian J Ophthalmol"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1007\/s00347-012-2678-9","article-title":"Prophylaxe und Management von Komplikationen bei perforierender Keratoplastik","volume":"110","author":"B Seitz","year":"2013","journal-title":"Ophthalmologe"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"1469","DOI":"10.1016\/j.ophtha.2006.11.022","article-title":"Morphometry of corneal endothelium in patients with corneal guttata","volume":"114","author":"C J Giasson","year":"2007","journal-title":"Ophthalmology"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1016\/0039-6257(78)90151-0","article-title":"Corneal dystrophies. 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