{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T10:28:14Z","timestamp":1783074494818,"version":"3.54.6"},"reference-count":28,"publisher":"Georg Thieme Verlag KG","issue":"04","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Laryngorhinootologie"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Zusammenfassung<\/jats:title><jats:p>Einleitung Die \u00c4tiopathogenese der chronischen Otitis media epitympanalis bzw. des Cholesteatoms und ihr proliferierender destruierender Verlauf mit m\u00f6glichen Komplikationen wie Destruktion der kn\u00f6chernen Strukturen mit H\u00f6rverlust, vestibul\u00e4rer Dysfunktion, Gesichtsnervenl\u00e4hmung und intrakraniellen Komplikationen sind immer noch ungekl\u00e4rt. Die Therapie der Wahl ist nach wie vor die operative Sanierung. Aktuelle Studien befassen sich immer mehr mit dem angeborenen Immunsystem.<\/jats:p><jats:p>Methoden Unsere Untersuchungen erfolgten im Mausmodell an WT-M\u00e4usen und immundefizienten KO-M\u00e4usen sowie an Gewebeproben vom Cholesteatom, gesunder Geh\u00f6rgangshaut und gesunder Mittelohrschleimhaut, die w\u00e4hrend sanierenden Ohroperationen entnommen wurden. Die Expressionsanalysen erfolgten auf Gen- und Proteinebene mit TNF als Major Target zur Therapieevaluation. Mittels TUNEL-F\u00e4rbung und Immunhistochemie an Kryoschnitten wurde die Apoptose-Rate durch TNF bestimmt.<\/jats:p><jats:p>Ergebnisse Das ungerichtet-expansive Cholesteatomwachstum zeigt ein immunmodulatorisches Profil mit Hoch- und Runterregulation von verschiedenen Gen-Netzwerken, vor allem Molek\u00fclen der TNF-Down- und -Upstream-Signalwege. Dabei wird TNF sowohl inflammatorisch als auch apoptotisch moduliert und eignet sich als m\u00f6glicher Therapieansatz in verschiedenen Modellen.<\/jats:p><jats:p>Schlussfolgerungen Es gibt Hinweise auf eine immunmodulatorische Regulation im Cholesteatom.<\/jats:p>","DOI":"10.1055\/a-1516-4447","type":"journal-article","created":{"date-parts":[[2021,7,7]],"date-time":"2021-07-07T22:53:53Z","timestamp":1625698433000},"page":"310-319","source":"Crossref","is-referenced-by-count":8,"title":["Immunmodulation im Cholesteatom"],"prefix":"10.1055","volume":"101","author":[{"given":"Anke","family":"Leichtle","sequence":"additional","affiliation":[{"name":"Klinik und Poliklinik f\u00fcr Hals-, Nasen- und Ohrenheilkunde, Universit\u00e4tsklinikum Schleswig-Holstein Campus \u2013 L\u00fcbeck HNO Klinik, L\u00fcbeck, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Leffers","sequence":"additional","affiliation":[{"name":"Klinik und Poliklinik f\u00fcr 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