{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T20:51:14Z","timestamp":1772830274762,"version":"3.50.1"},"reference-count":43,"publisher":"Georg Thieme Verlag KG","issue":"04","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tierarztl Prax Ausg G Grosstiere Nutztiere"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Zusammenfassung<\/jats:title><jats:p>\n                    Ziel Etablierung von Referenzintervallen f\u00fcr Mangan (Mn) im Serum, Plasma und Vollblut bei adulten, gesunden Warmblutpferden mit bekannter Mn-Aufnahme und Vergleich von 2 analytischen Methoden der Mn-Bestimmung im Blut.<\/jats:p><jats:p>\n                    Material und Methoden Zwischen Mai 2018 und November 2019 erfolgte auf 3 Gest\u00fcten bei 270 gesunden Pferden im Alter von 3\u201325 Jahren eine einmalige Blutprobenentnahme. In Vollblut, Plasma (Antikoagulans jeweils Lithium-Heparin [LH]) und Serum wurden die Mn-Konzentrationen mittels Massenspektrometrie mit induktiv gekoppeltem Plasma (ICP-MS) sowie mittels Atomabsorptionsspektrometrie (AAS) bestimmt. Die Referenzintervalle f\u00fcr jedes Substrat wurden nach den Empfehlungen der International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) und des Clinical and Laboratory Standards Institute (CLSI) berechnet (Bereich zwischen 2,5- und 97,5-Perzentil der analysierten Mn-Blutkonzentration). Zeitgleich mit der Blutprobenentnahme wurden repr\u00e4sentative Futterproben entnommen, um den Mn-Gehalt der korrespondierenden F\u00fctterung zu bestimmen.<\/jats:p><jats:p>\n                    Ergebnisse Die Pferde zeigten im Vollblut mit einer medianen Mn-Konzentration von 12,4 \u00b5g\/l (Referenzintervall: 4,99\u201325,1 \u00b5g\/l [AAS]; 5,99\u201325,3 \u00b5g\/l [ICP-MS]) signifikant h\u00f6here Mn-Konzentrationen (p &lt; 0,0001) als im korrespondierenden Serum (Median: 1,65 \u00b5g\/l, Referenzintervall: 0,60\u20133,50 \u00b5g\/l [AAS]; 1,11\u20132,96 \u00b5g\/l [ICP-MS]) oder LH-Plasma (Median: 1,35 \u00b5g\/l, Referenzintervall: 0,22\u20132,68 \u00b5g\/l [AAS]; 0,59\u20132,45 \u00b5g\/l [ICP-MS]). Im Methodenvergleich ergaben sich f\u00fcr LH-Plasma und Serum zwischen der AAS und der ICP-MS statistisch signifikante Abweichungen in den Mn-Bestimmungen, wohingegen sie f\u00fcr das Vollblut vergleichbare Ergebnisse lieferten.<\/jats:p><jats:p>\n                    Schlussfolgerung und klinische Relevanz Vollblut weist durchschnittlich 10-fach h\u00f6here Mn-Konzentrationen auf als Serum oder LH-Plasma. Bei der Bewertung von Mn-Konzentrationen im Blut muss daher ber\u00fccksichtigt werden, welches Probenmaterial analysiert wurde und welche Methode zur Anwendung kam, da in Serum und Plasma zwischen AAS und ICP-MS relevante Unterschiede auftreten k\u00f6nnen.<\/jats:p>","DOI":"10.1055\/a-1525-0868","type":"journal-article","created":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T22:33:51Z","timestamp":1629758031000},"page":"234-246","source":"Crossref","is-referenced-by-count":2,"title":["Mangankonzentrationen im Vollblut, Plasma und Serum adulter Warmblutpferde an 3 Standorten in Deutschland"],"prefix":"10.1055","volume":"49","author":[{"given":"Elena","family":"Theiner","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Tierern\u00e4hrung, Ern\u00e4hrungssch\u00e4den und Di\u00e4tetik, Universit\u00e4t Leipzig"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corinna","family":"Weber","sequence":"additional","affiliation":[{"name":"LABOKLIN GMBH & CO. KG, Bad Kissingen"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"LABOKLIN GMBH & CO. KG, Bad Kissingen"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica","family":"Venner","sequence":"additional","affiliation":[{"name":"Pferdeklinik Destedt GmbH"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingrid","family":"Vervuert","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Tierern\u00e4hrung, Ern\u00e4hrungssch\u00e4den und Di\u00e4tetik, Universit\u00e4t Leipzig"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2021,8,23]]},"reference":[{"issue":"05","key":"ref1","first-page":"200","article-title":"The importance of mineral elements for humans, domestic animals and plants: A review","volume":"4","author":"K O Soetan","year":"2010","journal-title":"Afr J Food Sci"},{"issue":"04\/05","key":"ref2","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1016\/j.mam.2005.07.003","article-title":"Nutritional aspects of manganese homeostasis","volume":"26","author":"J L Aschner","year":"2005","journal-title":"Mol Aspects Med"},{"issue":"01","key":"ref3","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1186\/2193-1801-3-343","article-title":"Concentrations of strontium, barium, cadmium, copper, zinc, manganese, chromium, antimony, selenium and lead in the equine liver and kidneys","volume":"3","author":"N Pa\u00dflack","year":"2014","journal-title":"SpringerPlus"},{"key":"ref4","volume-title":"Empfehlungen zur Energie- und N\u00e4hrstoffversorgung von Pferden. 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