{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T05:33:30Z","timestamp":1785389610697,"version":"3.55.0"},"reference-count":26,"publisher":"Georg Thieme Verlag KG","issue":"01","funder":[{"name":"Consejo Nacional de Ciencia y Tecnolog\u00eda (CONACyT) Proyecto de Ciencia B\u00e1sica","award":["A1-S-13540"],"award-info":[{"award-number":["A1-S-13540"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Drug Res (Stuttg)"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Several 4H-pyran derivatives were designed and synthesized previously as vasorelaxant agents for potential antihypertensive drugs. In this context, the objective of the present investigation was to determine the functional mechanism of vasorelaxant action of 6-amino-3-methyl-4-(2-nitrophenyl)-1,4-dihydropyrano[2,3-c]pyrazole-5-carbonitrile (1) and its in vivo antihypertensive effect. Thus, compound 1 showed significant vasorelaxant action on isolated aorta rat rings pre-contracted with serotonin or noradrenaline, and the effect was not endothelium-dependent. Compound 1 induced a significant relaxant effect when aortic rings were contracted with KCl (80\u2009mM), indicating that the main mechanism of action is related to L-type calcium channel blockade. Last was corroborated since compound 1 induced a significant concentration-dependent lowering of contraction provoked by cumulative CaCl2 adding. Moreover, compound 1 was capable to block the contraction induced by FPL 64176, a specific L-type calcium channel agonist, in a concentration-dependent manner. On the other hand, docking studies revealed that compound 1 interacts on two possible sites of the L-type calcium channel and it had better affinity energy (\u22127.80+\/\u22120.00\u2009kcal\/mol on the best poses) than nifedipine (\u22126.86+\/\u22120.14\u2009kcal\/mol). Finally, compound 1 (50\u2009mg\/kg) showed significant antihypertensive activity, lowering the systolic and diastolic blood pressure on spontaneously hypertensive rats (SHR) without modifying heart rate.<\/jats:p>","DOI":"10.1055\/a-1616-0156","type":"journal-article","created":{"date-parts":[[2021,10,19]],"date-time":"2021-10-19T00:40:25Z","timestamp":1634604025000},"page":"53-60","source":"Crossref","is-referenced-by-count":10,"title":["6-Amino-3-Methyl-4-(2-nitrophenyl)-1,4-Dihydropyrano[2,3-c]Pyrazole-5-Carbonitrile Shows Antihypertensive and Vasorelaxant Action via Calcium Channel Blockade"],"prefix":"10.1055","volume":"72","author":[{"given":"Samuel","family":"Estrada-Soto","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia, Universidad Aut\u00f3noma del Estado de Morelos, Cuernavaca, Morelos, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priscila","family":"Rend\u00f3n-Vallejo","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia, Universidad Aut\u00f3noma del Estado de Morelos, Cuernavaca, Morelos, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Villalobos-Molina","sequence":"additional","affiliation":[{"name":"Unidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Tlalnepantla, Estado de M\u00e9xico, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9sar","family":"Mill\u00e1n-Pacheco","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia, Universidad Aut\u00f3noma del Estado de Morelos, Cuernavaca, Morelos, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MiguelA.","family":"V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, Divisi\u00f3n de Ciencias Naturales y Exactas, Universidad de Guanajuato, Guanajuato, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Hern\u00e1ndez-Borja","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, Divisi\u00f3n de Ciencias Naturales y Exactas, Universidad de Guanajuato, Guanajuato, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuel","family":"Hern\u00e1ndez-N\u00fa\u00f1ez","sequence":"additional","affiliation":[{"name":"C\u00e1tedra CONACyT, Departamento de Recursos del Mar, Centro de Investigaci\u00f3n y de Estudios Avanzados del IPN, Unidad M\u00e9rida, Yucat\u00e1n, 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