{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T22:14:16Z","timestamp":1787004856385,"version":"build-2736575974"},"reference-count":46,"publisher":"Georg Thieme Verlag KG","issue":"13","funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"crossref","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","award":["315231\/2018-1"],"award-info":[{"award-number":["315231\/2018-1"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cannabis sativa is a millenary medicinal plant. However, contrary to worldwide paradigm-shifting, countries like Brazil still prohibit C. sativa cultivation and its medicinal use, even though many populations use aerial parts and roots of this plant for healthcare. As such, the objective of this work was to identify substances in the samples of the C. sativa roots, tracing a correlation with antitussive and expectorant effects. Therefore, samples of C. sativa roots were donated by the Pol\u00edcia Federal Brasileira, and its aqueous extract (AECsR) was prepared with subsequent lyophilization, to maintain the material stability. After that, the material was analyzed by LC-MS to observe its chemical profile. Four samples (AECsR-A, B, C, and D) were tested in animal models of citric acid-induced cough (0.4\u2009M) and phenol red expectoration (500\u2009mg\/kg). Using LC-MS it was possible to identify 5 molecules in C. sativa roots:\n\t\t\t\t\tp-coumaroyltyramine, tetrahydrocannabinol-C4, feruoiltyramine, anhydrocanabisativine, and cannabisativine. In experimental protocols, male mice (Mus musculus) were treated with samples of AECsR at doses of 12.5, 25, or 50\u2009mg\/kg regardless of the pharmacological test. In these tests, all samples showed the potential to treat cough and promote fluid expectoration, differing only in the dose at which these effects were observed. Therefore, the data showed that the C.\u00a0sativa roots of the Brazilian Northeast showed antitussive and expectorant effects, even with intense secondary metabolites\u02bc variation, which alters its potency, but not its effect. This highlights the importance of this medicinal plant for future therapy and corroborates to traditional use.<\/jats:p>","DOI":"10.1055\/a-1628-2299","type":"journal-article","created":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T19:20:50Z","timestamp":1635535250000},"page":"1223-1232","source":"Crossref","is-referenced-by-count":9,"title":["Chemical Analysis by LC-MS of Cannabis sativa Root Samples from Northeast Brazil and Evaluation of Antitussive and Expectorant Activities"],"prefix":"10.1055","volume":"88","author":[{"given":"Pedro Modesto Nascimento","family":"Menezes","sequence":"additional","affiliation":[{"name":"P\u00f3s-gradua\u00e7\u00e3o em Biotecnologia \u2013 RENORBIO, Universidade Federal Rural de Pernambuco\u2013 UFRPE, Recife\/PE \u2013 Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuella Chiara Valen\u00e7a","family":"Pereira","sequence":"additional","affiliation":[{"name":"P\u00f3s-gradua\u00e7\u00e3o em Biotecnologia \u2013 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Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarc\u00edsio C\u00edcero de Lima","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"P\u00f3s-gradua\u00e7\u00e3o em Bioci\u00eancias \u2013 PGB, Universidade Federal do Vale do S\u00e3o Francisco \u2013 UNIVASF, Petrolina\/PE \u2013 Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janaine Almeida","family":"Neto","sequence":"additional","affiliation":[{"name":"P\u00f3s-gradua\u00e7\u00e3o em Bioci\u00eancias \u2013 PGB, Universidade Federal do Vale do S\u00e3o Francisco \u2013 UNIVASF, Petrolina\/PE \u2013 Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciano Augusto de Araujo","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Universidade Federal do Vale do S\u00e3o Francisco \u2013 UNIVASF, Colegiado de Farm\u00e1cia (CFARM), Petrolina\/PE \u2013 Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabr\u00edcio Souza","family":"Silva","sequence":"additional","affiliation":[{"name":"P\u00f3s-gradua\u00e7\u00e3o em Biotecnologia \u2013 PPGBIOTEC, Universidade Estadual de Feira de Santana \u2013 UEFS, Feira de Santana\/BA \u2013 Brasil"},{"name":"Universidade Federal do Vale do S\u00e3o Francisco \u2013 UNIVASF, Colegiado de Farm\u00e1cia (CFARM), Petrolina\/PE \u2013 Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2403-1026","authenticated-orcid":false,"given":"Larissa Ara\u00fajo","family":"Rolim","sequence":"additional","affiliation":[{"name":"P\u00f3s-gradua\u00e7\u00e3o em Biotecnologia \u2013 RENORBIO, Universidade Federal Rural de Pernambuco\u2013 UFRPE, Recife\/PE \u2013 Brasil"},{"name":"P\u00f3s-gradua\u00e7\u00e3o em Bioci\u00eancias \u2013 PGB, Universidade Federal do Vale do S\u00e3o Francisco \u2013 UNIVASF, Petrolina\/PE \u2013 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