{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:33:30Z","timestamp":1740126810412,"version":"3.37.3"},"reference-count":37,"publisher":"Georg Thieme Verlag KG","issue":"01","funder":[{"name":"Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan","award":["15K10487"],"award-info":[{"award-number":["15K10487"]}]},{"name":"Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan","award":["17K11002"],"award-info":[{"award-number":["17K11002"]}]},{"name":"Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan","award":["19K18471."],"award-info":[{"award-number":["19K18471."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Horm Metab Res"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Incretins including glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1), which are secreted from the small intestine after oral food ingestion, are currently well-known to stimulate insulin secretion from pancreatic \u03b2-cells and used for the treatment of type 2 diabetes mellitus. We have previously reported that prostaglandin F2\u03b1 (PGF2\u03b1) stimulates the synthesis of interleukin-6 (IL-6) and osteoprotegerin in osteoblast-like MC3T3-E1 cells, and that IL-6 and osteoprotegerin release are mediated through the p44\/p42 mitogen-activated protein (MAP) kinase, p38 MAP kinase or stress-activated protein kinase\/c-Jun N-terminal kinase (SAPK\/JNK) pathways. In the present study, we investigated the effects of incretins including GLP-1 and GIP, on the PGF2\u03b1-induced synthesis of IL-6 and osteoprotegerin and examined the detailed mechanism in osteoblast-like MC3T3-E1 cells. We found that GIP and GLP-1 significantly stimulated the PGF2\u03b1-induced synthesis of IL-6 in osteoblast-like MC3T3-E1 cells. In addition, GIP and GLP-1 significantly enhanced the PGF2\u03b1-induced mRNA expression levels of IL-6. On the other hand, GIP and GLP-1 markedly stimulated the PGF2\u03b1-induced synthesis of osteoprotegerin. However, the phosphorylation of p44\/p42 MAP kinase, p38 MAP kinase, or JNK induced by PGF2\u03b1 was not affected by GIP or GLP-1. Therefore, these results strongly suggest that incretins enhance the PGF2\u03b1-induced synthesis of IL-6 and osteoprotegerin in osteoblast-like MC3T3-E1 cells. However, these syntheses are not mediated through p44\/p42 MAP kinase, p38 MAP kinase, or JNK pathways.<\/jats:p>","DOI":"10.1055\/a-1713-7967","type":"journal-article","created":{"date-parts":[[2022,1,5]],"date-time":"2022-01-05T23:30:26Z","timestamp":1641425426000},"page":"42-49","source":"Crossref","is-referenced-by-count":1,"title":["Incretins Enhance PGF2\u03b1-Induced Synthesis of IL-6 and Osteoprotegerin in Osteoblasts"],"prefix":"10.1055","volume":"54","author":[{"given":"Tomoyuki","family":"Hioki","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu, Japan"},{"name":"Department of Dermatology, Kizawa Memorial Hospital, Minokamo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1940-0085","authenticated-orcid":false,"given":"Gen","family":"Kuroyanagi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu, Japan"},{"name":"Department of Rehabilitation Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan"},{"name":"Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiko","family":"Fujita","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu, Japan"},{"name":"Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Go","family":"Sakai","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu, Japan"},{"name":"Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsu","family":"Kawabata","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu, Japan"},{"name":"Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Woo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu, Japan"},{"name":"Department of Anesthesiology and Pain Medicine, Gifu University Graduate School of Medicine, Gifu, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junko","family":"Tachi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu, Japan"},{"name":"Department of Anesthesiology and Pain Medicine, Gifu University Graduate School of Medicine, Gifu, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rie","family":"Matsushima-Nishiwaki","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroki","family":"Iida","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Pain Medicine, Gifu University Graduate School of Medicine, Gifu, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4834-6073","authenticated-orcid":false,"given":"Osamu","family":"Kozawa","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haruhiko","family":"Tokuda","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Gifu University Graduate 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