{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T21:47:02Z","timestamp":1764107222253,"version":"3.46.0"},"reference-count":39,"publisher":"Georg Thieme Verlag KG","issue":"S 01","funder":[{"name":"German Research Foundation and from the Herzstiftung","award":["BA5158\/4"],"award-info":[{"award-number":["BA5158\/4"]}]},{"name":"The state of Baden-W\u00fcrttemberg for coagulation research and blood donation service of German Red Cross"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hamostaseologie"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Coronavirus disease-2019 (COVID-19) is associated with increased thromboembolic complications. Long-term alteration in the coagulation system after acute COVID-19 infection is still a subject of research. Furthermore, the effect of sera from convalescent subjects on platelets is not known. In this study, we investigated platelet phenotype, coagulation, and fibrinolysis in COVID-19 convalescent plasma (CCP) donors and analyzed convalescent sera-induced effects on platelets. We investigated CCP donors who had a history of mild COVID-19 infection and donors who did not have COVID-19 were used as controls. We analyzed phosphatidylserine (PS) externalization, CD62p expression, and glycoprotein VI (GPVI) shedding both in platelet-rich plasma (PRP) and after incubation of washed healthy platelets with donors' sera using flow cytometry. Coagulation and fibrinolysis systems were assessed with thromboelastometry. Forty-seven CCP donors (22 males, 25 females; mean age (\u00b1SD): 41.4\u2009\u00b1\u200913.7 years) with a history of mild COVID-19 infection were included. Median duration after acute COVID-19 infection was 97 days (range, 34\u2013401). We did not find an increased PS externalization, CD62p expression, or GPVI shedding in platelets from CCP donors. Sera from CCP donors did not induce PS externalization or GPVI shedding in healthy platelets. Sera-induced CD62p expression was slightly, albeit statistically significantly, lower in CCP donors than in plasma donors without a history of COVID-19. One patient showed increased maximum clot firmness and prolonged lysis time in thromboelastometry. Our findings suggest that procoagulant platelet phenotype is not present after mild COVID-19. Furthermore, CCP sera do not affect the activation status of platelets.<\/jats:p>","DOI":"10.1055\/a-1797-0564","type":"journal-article","created":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T18:50:15Z","timestamp":1666810215000},"page":"S14-S23","source":"Crossref","is-referenced-by-count":2,"special_numbering":"S","title":["Platelets and Sera from Donors of Convalescent Plasma after Mild COVID-19 Show No Procoagulant Phenotype"],"prefix":"10.1055","volume":"42","author":[{"given":"G\u00fcnalp","family":"Uzun","sequence":"additional","affiliation":[{"name":"Centre for Clinical Transfusion Medicine, University Hospital of T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anurag","family":"Singh","sequence":"additional","affiliation":[{"name":"Institute for Clinical and Experimental Transfusion Medicine, Medical Faculty of T\u00fcbingen, University Hospital of T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wissam","family":"Abou-Khalel","sequence":"additional","affiliation":[{"name":"Centre for Clinical Transfusion Medicine, University Hospital of T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisann","family":"Pelzl","sequence":"additional","affiliation":[{"name":"Institute for Clinical and Experimental Transfusion Medicine, Medical Faculty of T\u00fcbingen, University Hospital of T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karoline","family":"Weich","sequence":"additional","affiliation":[{"name":"Centre for Clinical Transfusion Medicine, University Hospital of T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie","family":"Nowak-Harnau","sequence":"additional","affiliation":[{"name":"Centre for Clinical Transfusion Medicine, University Hospital of T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karina","family":"Althaus","sequence":"additional","affiliation":[{"name":"Centre for Clinical Transfusion Medicine, University Hospital of T\u00fcbingen, T\u00fcbingen, Germany"},{"name":"Institute for Clinical and Experimental Transfusion Medicine, Medical Faculty of T\u00fcbingen, University Hospital of T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Bugert","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, Hessen, Mannheim, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harald","family":"Kl\u00fcter","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, Hessen, Mannheim, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamam","family":"Bakchoul","sequence":"additional","affiliation":[{"name":"Centre for Clinical Transfusion Medicine, University Hospital of T\u00fcbingen, T\u00fcbingen, Germany"},{"name":"Institute for Clinical and Experimental Transfusion Medicine, Medical Faculty of T\u00fcbingen, University Hospital of T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2022,10,26]]},"reference":[{"issue":"07","key":"ref1","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1055\/s-0040-1712156","article-title":"Coronavirus disease 2019 coagulopathy: disseminated intravascular coagulation and thrombotic microangiopathy-either, neither, or both","volume":"46","author":"M Levi","year":"2020","journal-title":"Semin Thromb 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fibrinolysis in COVID-19","volume":"121","author":"S Hammer","year":"2021","journal-title":"Thromb Haemost"},{"issue":"03","key":"ref38","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1182\/bloodadvances.2021004816","article-title":"Altered fibrin clot structure and dysregulated fibrinolysis contribute to thrombosis risk in severe COVID-19","volume":"6","author":"M Wygrecka","year":"2022","journal-title":"Blood Adv"},{"issue":"01","key":"ref39","doi-asserted-by":"crossref","first-page":"13325","DOI":"10.1038\/s41598-021-92683-1","article-title":"Viscoelastic testing reveals normalization of the coagulation profile 12 weeks after severe COVID-19","volume":"11","author":"A Magomedov","year":"2021","journal-title":"Sci Rep"},{"issue":"07","key":"ref40","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1097\/01.mbc.0000245292.34150.df","article-title":"Global assessment of the coagulation status in type 2 diabetes mellitus using rotation thromboelastography","volume":"17","author":"B PS Y\u00fcrekli","year":"2006","journal-title":"Blood Coagul Fibrinolysis"}],"container-title":["H\u00e4mostaseologie"],"original-title":[],"language":"de","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-1797-0564.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T21:43:37Z","timestamp":1764107017000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-1797-0564"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10]]},"references-count":39,"journal-issue":{"issue":"S 01","published-online":{"date-parts":[[2022,10,26]]},"published-print":{"date-parts":[[2022,10]]}},"URL":"https:\/\/doi.org\/10.1055\/a-1797-0564","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["0720-9355","2567-5761"],"issn-type":[{"type":"print","value":"0720-9355"},{"type":"electronic","value":"2567-5761"}],"subject":[],"published":{"date-parts":[[2022,10]]}}}