{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T14:25:23Z","timestamp":1771079123949,"version":"3.50.1"},"reference-count":44,"publisher":"Georg Thieme Verlag KG","issue":"02","funder":[{"name":"Institutional program \u201cNuevas alternativas para el tratamiento de enfermedades infecciosas\u201d"},{"name":"Programa de Apoyo a Proyectos de Investigaci\u00f3n e Innovaci\u00f3n Tecnol\u00f3gica","award":["IG200321"],"award-info":[{"award-number":["IG200321"]}]},{"name":"Programa de Apoyo a Proyectos de Investigaci\u00f3n e Innovaci\u00f3n Tecnol\u00f3gica","award":["IN208020"],"award-info":[{"award-number":["IN208020"]}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"crossref","award":["284830"],"award-info":[{"award-number":["284830"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Plants of Tabernaemontana species have several pharmacological activities including antimicrobial effects. Amoebiasis continues to be a public health problem, with increasing\n\t\t\t\t\tevidence of resistance to metronidazole. In this study, we assessed the effect of the alkaloid fraction of T. arborea root bark and the alkaloids ibogaine and voacangine on the\n\t\t\t\t\tviability and infectivity of Entamoeba histolytica trophozoites. Cultures were exposed to 0.1\u200a\u2013\u200a10\u2009\u00b5g\/mL for 24, 48 and 72\u2009h, and viability was then determined using a tetrazolium dye\n\t\t\t\t\treduction assay and type of cellular death analyzed by flow cytometry. Results showed that the alkaloid fraction, but mainly ibogaine and voacangine alkaloids, exhibited potent\n\t\t\t\t\tdose-dependent anti-amoebic activity at 24\u2009h post-exposure (IC50 4.5 and 8.1\u2009\u00b5M, respectively), comparable to metronidazole (IC50 6.8\u2009\u00b5M). However, the effect decreased\n\t\t\t\t\tafter 48 and 72\u2009h of exposure to concentrations below 10\u2009\u00b5g\/mL, suggesting that the alkaloids probably were catabolized to less active derivatives by the trophozoites. The treatment of\n\t\t\t\t\ttrophozoites with the IC50\u2009s for 24\u2009h induced significant morphological changes in the trophozoites, slight increase in granularity, and death by apoptonecrosis. The capacity of\n\t\t\t\t\t\tT. arborea alkaloids to inhibit the development of amoebic liver abscesses in hamsters was evaluated. Results showed that even when the treatments reduced the number of amoebic\n\t\t\t\t\ttrophozoites in tissue sections of livers, they were unable to limit the formation of abscesses, suggesting their rapid processing to inactive metabolites. This work leaves open the\n\t\t\t\t\tpossibility of using Tabernaemontana alkaloids as a new alternative for amoebiasis control.<\/jats:p>","DOI":"10.1055\/a-1809-1157","type":"journal-article","created":{"date-parts":[[2022,3,26]],"date-time":"2022-03-26T00:18:21Z","timestamp":1648253901000},"page":"148-157","source":"Crossref","is-referenced-by-count":6,"title":["Potent Anti-amoebic Effects of Ibogaine, Voacangine and the Root Bark Alkaloid Fraction of Tabernaemontana arborea"],"prefix":"10.1055","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1055-5774","authenticated-orcid":false,"given":"Julio C\u00e9sar","family":"Carrero","sequence":"additional","affiliation":[{"name":"Departamento de Inmunolog\u00eda, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Violeta","family":"Curay-Herrera","sequence":"additional","affiliation":[{"name":"Escuela Profesional de Ciencias Biol\u00f3gicas, Facultad de Ciencias, Universidad de Piura, Piura, Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lysette","family":"Chac\u00f3n-Ni\u00f1o","sequence":"additional","affiliation":[{"name":"Departamento de Inmunolog\u00eda, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felix","family":"Krengel","sequence":"additional","affiliation":[{"name":"Departamento de Ecolog\u00eda y Recursos Naturales, Facultad de Ciencias, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia-Laura","family":"Guzm\u00e1n-Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"CONACyT-Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayra","family":"Silva-Miranda","sequence":"additional","affiliation":[{"name":"CONACyT-Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luisa-Carolina","family":"Gonz\u00e1lez-Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n \u201cAn\u00e1lisis de Muestras Biol\u00f3gicas y Forenses\u201d, Carrera Laboratorio Cl\u00ednico, Facultad de Ciencias de la Salud, Universidad Nacional de Chimborazo, Riobamba,\n\t\t\t\t\tEcuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ra\u00fal J.","family":"Bobes","sequence":"additional","affiliation":[{"name":"Departamento de Inmunolog\u00eda, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clara","family":"Espitia","sequence":"additional","affiliation":[{"name":"Departamento de Inmunolog\u00eda, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"Reyes-Chilpa","sequence":"additional","affiliation":[{"name":"Departamento de Productos Naturales, Instituto de Qu\u00edmica, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan-Pedro","family":"Laclette","sequence":"additional","affiliation":[{"name":"Departamento de Inmunolog\u00eda, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2022,7,22]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"2095","DOI":"10.1016\/S0140-6736(12)61728-0","article-title":"Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: A systematic analysis for the Global Burden of Disease Study 2010","volume":"380","author":"R Lozano","year":"2012","journal-title":"Lancet"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.bjid.2012.08.021","article-title":"Prevalence and characters of Entamoeba histolytica infection in Saudi infants 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