{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T10:35:36Z","timestamp":1785494136827,"version":"3.56.0"},"reference-count":40,"publisher":"Georg Thieme Verlag KG","issue":"11","funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","award":["200175\/2018-1"],"award-info":[{"award-number":["200175\/2018-1"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","award":["401490\/2014-9"],"award-info":[{"award-number":["401490\/2014-9"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Periodontal diseases are a global oral health problem affecting almost 10% of the global population. Porphyromonas gingivalis is one of the main bacteria involved in the initiation\n          and progression of inflammatory processes as a result of the action of the cysteine proteases lysin- and arginine-gingipain. Surelease\/polycarbophil microparticles containing a lyophilized\n          proanthocyanidin-enriched fraction from the rhizomes of Limonium brasiliense, traditionally named \u201cbaicuru\u201d (ethyl acetate fraction), were manufactured. The ethyl acetate\n          fraction was characterized by UHPLC by the presence of samarangenins A and B (12.10\u2009\u00b1\u20090.07 and 21.05\u2009\u00b1\u20090.44%, respectively) and epigallocatechin-3-O-gallate (13.44\u2009\u00b1\u20090.27%).\n          Physiochemical aspects of Surelease\/polycarbophil microparticles were characterized concerning particle size, zeta potential, entrapment efficiency, ethyl acetate fraction release, and\n          mucoadhesion. Additionally, the presence of the ethyl acetate fraction-loaded microparticles was performed concerning potential influence on viability of human buccal KB cells, P.\n          gingivalis adhesion to KB cells, gingipain activity, and P. gingivalis biofilm formation. In general, all Surelease\/polycarbophil microparticles tested showed strong adhesion to\n          porcine cheek mucosa (93.1\u2009\u00b1\u20094.2% in a 30-min test), associated with a prolonged release of the ethyl acetate fraction (up to 16.5\u2009\u00b1\u20090.8% in 24\u2009h). Preincubation of KB cells with\n          Surelease\/polycarbophil microparticles (25\u2009\u00b5g\/mL) resulted in an up to 93\u2009\u00b1\u20092% reduced infection rate by P. gingivalis. Decreased activity of the P. gingivalis-specific\n          virulence factors lysin- and arginine-gingipain proteases by Surelease\/polycarbophil microparticles was confirmed. Surelease\/polycarbophil microparticles decreased biofilm formation of P.\n          gingivalis (97\u2009\u00b1\u20092% at 60\u2009\u00b5g\/mL). Results from this study prove the promising activity of Surelease\/polycarbophil microparticles containing ethyl acetate fraction microparticles as a\n          prophylaxis strategy to prevent the recurrence of P. gingivalis.\n        <\/jats:p>","DOI":"10.1055\/a-1858-6898","type":"journal-article","created":{"date-parts":[[2022,5,22]],"date-time":"2022-05-22T22:47:55Z","timestamp":1653259675000},"page":"1074-1086","source":"Crossref","is-referenced-by-count":4,"title":["Microstructured Polymer System Containing Proanthocyanidin-Enriched Extract from Limonium brasiliense as a Prophylaxis Strategy to Prevent Recurrence of Porphyromonas\n          gingivalis"],"prefix":"10.1055","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5296-8078","authenticated-orcid":false,"given":"Fernanda","family":"Pilatti","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences, Palafito, Universidade Estadual de Maring\u00e1, Maring\u00e1, Brazil"},{"name":"Institute of Pharmaceutical Biology and Phytochemistry, University of M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8241-7878","authenticated-orcid":false,"given":"Raquel","family":"Isolani","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences, Palafito, Universidade Estadual de Maring\u00e1, Maring\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5399-1969","authenticated-orcid":false,"given":"Larissa","family":"Valone","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences, Palafito, Universidade Estadual de Maring\u00e1, Maring\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0088-8252","authenticated-orcid":false,"given":"Mariana Nascimento","family":"de Paula","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences, Palafito, Universidade Estadual de Maring\u00e1, Maring\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8288-297X","authenticated-orcid":false,"given":"Angelo de Oliveira","family":"Caleare","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences, Palafito, Universidade Estadual de Maring\u00e1, Maring\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8364-0449","authenticated-orcid":false,"given":"Sabrina Barbosa de Souza","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences, Palafito, Universidade Estadual de Maring\u00e1, Maring\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4838-5742","authenticated-orcid":false,"given":"Marcos Luciano","family":"Bruschi","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences, Palafito, Universidade Estadual de Maring\u00e1, Maring\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8602-035X","authenticated-orcid":false,"given":"Daniela Cristina","family":"de Medeiros Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences, Palafito, Universidade Estadual de Maring\u00e1, Maring\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5364-609X","authenticated-orcid":false,"given":"Terezinha Aparecida","family":"Guedes","sequence":"additional","affiliation":[{"name":"Department of Statistics, Universidade Estadual de Maring\u00e1, Maring\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0886-8384","authenticated-orcid":false,"given":"Andreas","family":"Hensel","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Biology and Phytochemistry, University of M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7532-3395","authenticated-orcid":false,"given":"Jo\u00e3o Carlos Palazzo","family":"de Mello","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences, Palafito, Universidade Estadual de Maring\u00e1, Maring\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2022,9,28]]},"reference":[{"key":"ref3","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.atherosclerosis.2017.05.009","article-title":"Oral health and 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