{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T17:23:07Z","timestamp":1774632187743,"version":"3.50.1"},"reference-count":49,"publisher":"Georg Thieme Verlag KG","issue":"05","funder":[{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"crossref","award":["2021CFB227"],"award-info":[{"award-number":["2021CFB227"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Special project of guiding local science and technology development by the central government of Hubei Province","award":["2020ZYYD030"],"award-info":[{"award-number":["2020ZYYD030"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Acute lung injury (ALI) is one of the representative \u201clung heat syndromes\u201d in traditional Chinese medicine (TCM). Scutellaria baicalensis is an herbal medicine used in TCM for\n          treating lung diseases, due to its remarkable anti-inflammatory and antiviral effects. When used in TCM, S. baicalensis root is divided into two categories: S. baicalensis\n          pith-not-decayed root (SN) and S. baicalensis pith-decayed root (SD). Compared to SN, SD has a better effect on lung diseases. We constructed a lipopolysaccharide (LPS)-induced acute\n          lung injury (ALI) mouse model to study the pharmacodynamic mechanism of SD. The ethanolic extract of Scutellaria baicalensis pith-decayed root (EESD) significantly affected\n          LPS-induced ALI by reducing alveolar interstitial thickening, pulmonary edema, and other pathological symptoms, decreasing the infiltration of inflammatory cells, especially macrophages, and\n          inhibiting IL-1\u03b2, TNF-\u03b1, and IL-6 transcription and translation. Furthermore, in the THP-1 macrophage model induced by LPS, EESD inhibited the expression of phosphorylated\n          nuclear factor inhibitory protein alpha (p-I\u03baB\u03b1), phosphorylated nuclear factor-\u03baB P65 (p-p65), cleaved-caspase-1, cleaved-IL-1\u03b2 protein, and the release of\n          inflammatory factors in the NF-\u03baB\/NLRP3 pathway, inhibiting macrophage function. In vivo experiments yielded similar results. Therefore, the present study clarified the\n          potential of EESD in the treatment of ALI and revealed its potential pharmacodynamic mechanism by inhibiting the NF-\u03baB\/NLRP3 inflammasome pathway and suppressing the pro-inflammatory\n          phenotype activation of lung tissue macrophages.<\/jats:p>","DOI":"10.1055\/a-1878-5704","type":"journal-article","created":{"date-parts":[[2022,6,18]],"date-time":"2022-06-18T22:32:21Z","timestamp":1655591541000},"page":"493-507","source":"Crossref","is-referenced-by-count":15,"title":["Scutellaria baicalensis Pith-decayed Root Inhibits Macrophage-related Inflammation Through the NF-\u03baB\/NLRP3 Pathway to Alleviate LPS-induced Acute Lung\n          Injury"],"prefix":"10.1055","volume":"89","author":[{"given":"Fanglei","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, P.\u200aR. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang","family":"Ke","sequence":"first","affiliation":[{"name":"School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, P.\u200aR. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongshi","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, P.\u200aR. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kang","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, P.\u200aR. China"},{"name":"Hubei Research Center of Chinese Materia Medica Processing Engineering and Technology, Hubei University of Chinese Medicine, Wuhan, P.\u200aR. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, P.\u200aR. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanju","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, P.\u200aR. China"},{"name":"Hubei Research Center of Chinese Materia Medica Processing Engineering and Technology, Hubei University of Chinese Medicine, Wuhan, P.\u200aR. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiyuan","family":"Tu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, P.\u200aR. China"},{"name":"Hubei Research Center of Chinese Materia Medica Processing Engineering and Technology, Hubei University of Chinese Medicine, Wuhan, P.\u200aR. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2022,11,7]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"76","DOI":"10.16448\/j.cjtcm.2008.01.014","article-title":"Discussion on pathogenesis and treatment of acute lung injury in Traditional Chinese Medicine","volume":"20","author":"X Z Jin","year":"2008","journal-title":"CJTCM"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"615","DOI":"10.7861\/clinmedicine.11-6-615","article-title":"Acute lung injury","volume":"11","author":"D Parekh","year":"2011","journal-title":"Clin Med (Lond)"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"105224","DOI":"10.1016\/j.phrs.2020.105224","article-title":"Natural product derived phytochemicals in managing acute lung injury by multiple mechanisms","volume":"163","author":"Y Q He","year":"2021","journal-title":"Pharmacol Res"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"100777","DOI":"10.1016\/j.cpsurg.2020.100777","article-title":"Acute lung injury","volume":"57","author":"N T Mowery","year":"2020","journal-title":"Curr Probl Surg"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1097\/SHK.0000000000001598","article-title":"Cell-cell interaction mechanisms in acute lung injury","volume":"55","author":"H Zhou","year":"2021","journal-title":"Shock"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"61","DOI":"10.3892\/ijmm.2019.4402","article-title":"HMGB1 participates in LPS-induced acute lung injury by activating the AIM2 inflammasome in macrophages and inducing polarization of M1 macrophages via TLR2, TLR4, and RAGE\/NFkappaB\n          signaling pathways","volume":"45","author":"J Wang","year":"2020","journal-title":"Int J Mol Med"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"664","DOI":"10.1038\/s41419-019-1900-y","article-title":"Efficacy of pulmonary transplantation of engineered macrophages secreting IL-4 on acute lung injury in C57BL\/6J mice","volume":"10","author":"H Liu","year":"2019","journal-title":"Cell Death Dis"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"1664913","DOI":"10.1155\/2018\/1264913","article-title":"The role of macrophages in the pathogenesis of ALI\/ARDS","volume":"2018","author":"X Huang","year":"2018","journal-title":"Mediators Inflamm"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1093\/intimm\/dxy054","article-title":"Macrophages in inflammation, repair and regeneration","volume":"30","author":"Y Oishi","year":"2018","journal-title":"Int Immunol"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"245","DOI":"10.5858\/arpa.2015-0519-RA","article-title":"Acute lung injury: A Clinical and molecular review","volume":"140","author":"Y Butt","year":"2016","journal-title":"Arch Pathol Lab Med"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1146\/annurev-pathmechdis-012418-012718","article-title":"Diversity, mechanisms, and significance of macrophage plasticity","volume":"15","author":"M Locati","year":"2020","journal-title":"Annu Rev Pathol"},{"key":"ref12","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1142\/S0192415X17500100","article-title":"Scutellaria baicalensis ameliorates acute lung injury by suppressing inflammation in vitro and in vivo","volume":"45","author":"J J Chen","year":"2017","journal-title":"Am J Chin Med"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/j.phymed.2018.12.009","article-title":"In vivo effect of quantified flavonoids-enriched extract of Scutellaria baicalensis root on acute lung injury induced by influenza A virus","volume":"57","author":"H J Zhi","year":"2019","journal-title":"Phytomedicine 57"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"112876","DOI":"10.1016\/j.jpba.2019.112876","article-title":"Exploring the effective materials of flavonoids-enriched extract from Scutellaria baicalensis roots based on the metabolic activation in influenza A virus induced acute lung\n          injury","volume":"177","author":"H Zhi","year":"2020","journal-title":"J Pharm Biomed Anal"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"2431","DOI":"10.3390\/molecules24132431","article-title":"Comparative investigation for rotten Xylem (Kuqin) and strip types (Tiaoqin) of Scutellaria baicalensis georgi based on fingerprinting and chemical pattern recognition","volume":"24","author":"X Cao","year":"2019","journal-title":"Molecules"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1016\/S1875-5364(17)30077-8","article-title":"Differences in anti-inflammatory effects between two specifications of Scutellariae Radix in LPS-induced macrophages in vitro","volume":"15","author":"Q Y Chen","year":"2017","journal-title":"Chin J Nat Med"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"4064","DOI":"10.7501\/j.issn.0253-2670.2018.17.015","article-title":"Pharmacodynamic difference of Scutellaria baicalensis pith-nodecayed and Scutellaria baicalensis pith-decayed on pneumonia based on antibacterial activity and pathological\n          parameters","volume":"49","author":"J W Zhai","year":"2018","journal-title":"Zhong Cao Yao"},{"key":"ref18","doi-asserted-by":"publisher","first-page":"2756","DOI":"10.3969\/j.issn.1001-1528.2020.10.042","article-title":"Comparative study on the protective effects of alcohol extracts of Kuqin and Ziqin on LPS-induced acute lung injury","volume":"42","author":"J H Hu","year":"2020","journal-title":"Zhong Cheng Yao"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"113596","DOI":"10.1016\/j.jep.2020.113596","article-title":"Investigation on the function tropism of Tiaoqin and Kuqin (different specification of Scutellaria baicalensis) by comparing their curative effect on different febrile disease\n          model","volume":"268","author":"Y Ren","year":"2021","journal-title":"J Ethnopharmacol"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"640","DOI":"10.1016\/j.immuni.2015.03.007","article-title":"Lipopolysaccharide induces alveolar macrophage necrosis via CD14 and the P2X7 receptor leading to interleukin-1alpha release","volume":"42","author":"J Dagvadorj","year":"2015","journal-title":"Immunity"},{"key":"ref21","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1016\/j.it.2021.06.001","article-title":"The \u2018cytokine storm\u2019: molecular mechanisms and therapeutic prospects","volume":"42","author":"R Karki","year":"2021","journal-title":"Trends Immunol"},{"key":"ref22","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1159\/000510269","article-title":"PolyADP-ribosylation of NFATc3 and NF-kappaB transcription factors modulate macrophage inflammatory gene expression in LPS-induced acute lung injury","volume":"13","author":"Y Nie","year":"2021","journal-title":"J Innate Immun"},{"key":"ref23","doi-asserted-by":"publisher","first-page":"2689","DOI":"10.3892\/mmr.2017.8120","article-title":"Protective effect of baicalin against severe burninduced remote acute lung injury in rats","volume":"17","author":"C Bai","year":"2018","journal-title":"Mol Med Rep"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"2006","DOI":"10.1007\/s10753-014-9932-z","article-title":"Wogonoside ameliorates lipopolysaccharide-induced acute lung injury in mice","volume":"37","author":"L Zhang","year":"2014","journal-title":"Inflammation"},{"key":"ref25","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1016\/j.jep.2014.02.010","article-title":"Baicalein, an active component of Scutellaria baicalensis, protects against lipopolysaccharide-induced acute lung injury in rats","volume":"153","author":"C L Tsai","year":"2014","journal-title":"J Ethnopharmacol"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1111\/imm.12305","article-title":"Wogonin prevents lipopolysaccharide-induced acute lung injury and inflammation in mice via peroxisome proliferator-activated receptor gamma-mediated attenuation of the nuclear\n          factor-kappaB pathway","volume":"143","author":"J Yao","year":"2014","journal-title":"Immunology"},{"key":"ref27","doi-asserted-by":"publisher","first-page":"e47403","DOI":"10.1371\/journal.pone.0047403","article-title":"Oroxylin-A rescues LPS-induced acute lung injury via regulation of NF-kappaB signaling pathway in rodents","volume":"7","author":"T L Tseng","year":"2012","journal-title":"PLoS One"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"773","DOI":"10.1586\/ers.10.71","article-title":"The value of the lipopolysaccharide-induced acute lung injury model in respiratory medicine","volume":"4","author":"H Chen","year":"2010","journal-title":"Expert Rev Respir Med"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"411","DOI":"10.51620\/0869-2084-2021-66-7-411-416","article-title":"Role of lungs in the hemostasis system (review of literature)","volume":"66","author":"A N Aripov","year":"2021","journal-title":"Klin Lab Diagn"},{"key":"ref30","doi-asserted-by":"publisher","first-page":"3251","DOI":"10.1002\/ptr.6499","article-title":"Acacia catechu (L.f.) Willd and Scutellaria baicalensis Georgi extracts suppress LPS-induced pro-inflammatory responses through NF-small ka, CyrillicB, MAPK, and PI3K-Akt signaling\n          pathways in alveolar epithelial type II cells","volume":"33","author":"T Feng","year":"2019","journal-title":"Phytother Res"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1016\/j.intimp.2019.04.046","article-title":"Baicalin alleviated APEC-induced acute lung injury in chicken by inhibiting NF-kappaB pathway activation","volume":"72","author":"L Y Peng","year":"2019","journal-title":"Int Immunopharmacol"},{"key":"ref32","doi-asserted-by":"publisher","first-page":"106024","DOI":"10.1016\/j.intimp.2019.106024","article-title":"Anti-inflammatory effect of Yam Glycoprotein on lipopolysaccharide-induced acute lung injury via the NLRP3 and NF-kappaB\/TLR4 signaling pathway","volume":"81","author":"X Niu","year":"2020","journal-title":"Int Immunopharmacol"},{"key":"ref33","doi-asserted-by":"publisher","first-page":"896","DOI":"10.1016\/j.cell.2015.12.057","article-title":"NF-kappaB restricts inflammasome activation via elimination of damaged mitochondria","volume":"164","author":"Z Zhong","year":"2016","journal-title":"Cell"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"749","DOI":"10.2147\/JIR.S277457","article-title":"Role of caspase-1 in the pathogenesis of inflammatory-associated chronic noncommunicable diseases","volume":"13","author":"M D Molla","year":"2020","journal-title":"J Inflamm Res"},{"key":"ref35","doi-asserted-by":"publisher","first-page":"152997","DOI":"10.1016\/j.phymed.2019.152997","article-title":"Inhibition of myeloid differentiation factor 2 by baicalein protects against acute lung injury","volume":"63","author":"H Chen","year":"2019","journal-title":"Phytomedicine"},{"key":"ref36","doi-asserted-by":"publisher","first-page":"e12868","DOI":"10.1111\/cpr.12868","article-title":"Wedelolactone facilitates Ser\/Thr phosphorylation of NLRP3 dependent on PKA signalling to block inflammasome activation and pyroptosis","volume":"53","author":"H Pan","year":"2020","journal-title":"Cell Prolif"},{"key":"ref37","doi-asserted-by":"publisher","first-page":"2529","DOI":"10.4049\/jimmunol.1002720","article-title":"Differential expression of NLRP3 among hematopoietic cells","volume":"186","author":"G Guarda","year":"2011","journal-title":"J Immunol"},{"key":"ref38","doi-asserted-by":"publisher","first-page":"2101","DOI":"10.13863\/j.issn1001-4454.2017.09.024","article-title":"Multi-component content determination combined with UPLC fingerprints and its pattern discrimination evaluation of Scutellaria baicalensis pith-nodecayed and Scutellaria\n          baicalensis pith-decayed","volume":"40","author":"J W Zhao","year":"2017","journal-title":"Zhong Yao Cai"},{"key":"ref39","doi-asserted-by":"publisher","first-page":"392","DOI":"10.3390\/molecules21030392","article-title":"Trapa japonica pericarp extract reduces LPS-induced inflammation in macrophages and acute lung injury in mice","volume":"21","author":"Y S Kim","year":"2016","journal-title":"Molecules"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"987","DOI":"10.1007\/s10753-019-00960-z","article-title":"Umbilical cord blood mesenchymal stem cells enhance lipopolysaccharide-induced IL-10 and IL-37 production in THP-1 Cells","volume":"42","author":"T Zhou","year":"2019","journal-title":"Inflammation"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"106786","DOI":"10.1016\/j.dib.2021.106786","article-title":"Dataset on the differentiation of THP-1 monocytes to LPS inducible adherent macrophages and their capacity for NO\/iNOS signaling","volume":"35","author":"A Ozleyen","year":"2021","journal-title":"Data Brief"},{"key":"ref42","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/j.jep.2017.04.008","article-title":"Chinese medicine Bu-Fei decoction attenuates epithelial-mesenchymal transition of non-small cell lung cancer via inhibition of transforming growth factor beta1 signaling pathway in\n          vitro and in vivo","volume":"204","author":"X R He","year":"2017","journal-title":"J Ethnopharmacol"},{"key":"ref43","doi-asserted-by":"publisher","first-page":"7483278","DOI":"10.1155\/2020\/7483278","article-title":"Bufei decoction alleviated bleomycin-induced idiopathic pulmonary fibrosis in mice by anti-inflammation","volume":"2020","author":"S Yang","year":"2020","journal-title":"Evid Based Complement Alternat Med"},{"key":"ref44","doi-asserted-by":"publisher","first-page":"113925","DOI":"10.1016\/j.jep.2021.113925","article-title":"Atractylodes oil alleviates diarrhea-predominant irritable bowel syndrome by regulating intestinal inflammation and intestinal barrier via SCF\/c-kit and MLCK\/MLC2 pathways","volume":"272","author":"Y Xie","year":"2021","journal-title":"J Ethnopharmacol"},{"key":"ref45","doi-asserted-by":"publisher","first-page":"665376","DOI":"10.3389\/fphar.2021.665376","article-title":"Atractylodin attenuates dextran sulfate sodium-induced colitis by alleviating gut microbiota dysbiosis and inhibiting inflammatory response through the MAPK pathway","volume":"12","author":"L Qu","year":"2021","journal-title":"Front Pharmacol"},{"key":"ref46","doi-asserted-by":"publisher","first-page":"110130","DOI":"10.1016\/j.biopha.2020.110130","article-title":"Effects of atractylodes oil on inflammatory response and serum metabolites in adjuvant arthritis rats","volume":"127","author":"Q Linghang","year":"2020","journal-title":"Biomed Pharmacother"},{"key":"ref47","doi-asserted-by":"publisher","first-page":"D607","DOI":"10.1093\/nar\/gky1131","article-title":"STRING v11: Protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets","volume":"47","author":"D Szklarczyk","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"ref48","doi-asserted-by":"publisher","first-page":"1.30.1","DOI":"10.1002\/cpbi.5","article-title":"The GeneCards Suite: From gene data mining to disease genome sequence analyses","volume":"54","author":"G Stelzer","year":"2016","journal-title":"Curr Protoc Bioinformatics"},{"key":"ref49","doi-asserted-by":"publisher","first-page":"1523","DOI":"10.1038\/s41467-019-09234-6","article-title":"Metascape provides a biologist-oriented resource for the analysis of systems-level datasets","volume":"10","author":"Y Zhou","year":"2019","journal-title":"Nat Commun"}],"container-title":["Planta Medica"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-1878-5704.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T00:35:25Z","timestamp":1682555725000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-1878-5704"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,18]]},"references-count":49,"journal-issue":{"issue":"05","published-online":{"date-parts":[[2023,4,26]]},"published-print":{"date-parts":[[2023,4]]}},"URL":"https:\/\/doi.org\/10.1055\/a-1878-5704","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["0032-0943","1439-0221"],"issn-type":[{"value":"0032-0943","type":"print"},{"value":"1439-0221","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2022,6,18]]},"published":{"date-parts":[[2022,6,18]]}}}