{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T09:45:50Z","timestamp":1785577550229,"version":"3.56.0"},"reference-count":27,"publisher":"Georg Thieme Verlag KG","issue":"09","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Horm Metab Res"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Preeclampsia (PE) may pose significant adverse effects on pregnant women.\n                    Dysregulation of angiogenesis, trophoblast invasion, and proliferation are known\n                    to be associated with PE development and progression. Fms related tyrosine\n                    kinase 1 (FLT1), an anti-angiogenic factor, is consistently upregulated in PE\n                    patients. Recent papers highlight that aberrant miR-30a-3p expression\n                    contributes to PE development. More effects are needed to assess the biological\n                    function of placental miR-30a-3p in PE. The soluble FLT1 (sFLT1) and FLT1 levels\n                    were tested by ELISA assay and Western blotting assay. mRNA levels were measured\n                    by RT-qPCR assay. Colony formation and MTT assays were applied to assess the\n                    effect of miR-30a-3p on trophoblast cell proliferation. The serum sFLT1 and\n                    placental FLT1 levels were substantially high in patients with PE. Using miRNA\n                    microarray assay, we identified miR-30a-3p upregulation in PE patients\u2019\n                    placenta tissues. We further confirmed that miR-30a-3p binds to the\n                    3\u2032-UTR of FLT1 gene and positively regulate its expression. Forcing\n                    miR-30a-3p expression inhibited trophoblast cell proliferation and vice versa.\n                    In conclusion, persistent high levels of FLT1 and miR-30a-3p may pose adverse\n                    effects on angiogenesis and trophoblast proliferation in placenta of PE\n                    patients. Therefore, targeting FLT1 and miR-30a-3p may serve as ideal strategies\n                    for managing patients with PE.<\/jats:p>","DOI":"10.1055\/a-1880-1126","type":"journal-article","created":{"date-parts":[[2022,8,18]],"date-time":"2022-08-18T22:42:45Z","timestamp":1660862565000},"page":"633-640","source":"Crossref","is-referenced-by-count":11,"title":["MiR-30a-3p Targeting FLT1 Modulates Trophoblast Cell Proliferation in\n                    the Pathogenesis of Preeclampsia"],"prefix":"10.1055","volume":"54","author":[{"given":"Yuping","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, The Affiliated Yantai\n                    Yuhuangding Hospital of Qingdao University, Yantai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanlan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, The Affiliated Yantai\n                    Yuhuangding Hospital of Qingdao University, Yantai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, The Affiliated Yantai\n                    Yuhuangding Hospital of Qingdao University, Yantai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenwen","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, The Affiliated Yantai\n                    Yuhuangding Hospital of Qingdao University, Yantai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2022,8,18]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"71","DOI":"10.5830\/CVJA-2016-009","article-title":"Pre-eclampsia: its pathogenesis and pathophysiolgy","volume":"27","author":"P Gathiram","year":"2016","journal-title":"Cardiovasc J 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Genet"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1172\/JCI17189","article-title":"Excess placental soluble fms-like tyrosine kinase 1 (sFlt1) may contribute to\n                    endothelial dysfunction, hypertension, and proteinuria in preeclampsia","volume":"111","author":"S E Maynard","year":"2003","journal-title":"J Clin Invest"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"E1051","DOI":"10.1210\/jc.2011-3131","article-title":"Preeclampsia up-regulates angiogenesis-associated microRNA (i.\u2009e.,\n                    miR-17, -20a, and -20b) that target ephrin-B2 and EPHB4 in human placenta","volume":"97","author":"W Wang","year":"2012","journal-title":"J Clin Endocrinol Metab"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"3470","DOI":"10.1210\/jc.2017-00849","article-title":"Preeclampsia downregulates microRNAs in fetal endothelial cells: roles of\n                    miR-29a\/c-3p in endothelial 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Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-1880-1126.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T23:37:10Z","timestamp":1662593830000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-1880-1126"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,18]]},"references-count":27,"journal-issue":{"issue":"09","published-online":{"date-parts":[[2022,9,7]]},"published-print":{"date-parts":[[2022,9]]}},"URL":"https:\/\/doi.org\/10.1055\/a-1880-1126","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["0018-5043","1439-4286"],"issn-type":[{"value":"0018-5043","type":"print"},{"value":"1439-4286","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,18]]}}}