{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T10:49:33Z","timestamp":1769165373798,"version":"3.49.0"},"reference-count":71,"publisher":"Georg Thieme Verlag KG","issue":"04","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Pediatr Surg"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Objective\u2003Hirschsprung's disease (HSCR) is a congenital intestinal neurodevelopmental disorder characterized by the absence of enteric ganglion cells in the distal colon. Although Hirschsprung-associated enterocolitis (HAEC) is the most frequent life-threatening complication in HSCR, to date reliable biomarkers predicting the likelihood of HAEC are yet to be established. We established a three-center retrospective study including 104 HSCR patients surgically treated between 1998 and 2019.<\/jats:p><jats:p>\n          Materials and Methods\u2003Patient-derived cryopreserved or paraffin-preserved colonic tissue at surgery was analyzed via \u03b2III-tubulin immunohistochemistry. We subsequently determined extrinsic mucosal nerve fiber density in resected rectosigmoid specimens and classified HSCR patients accordingly into nerve fiber-high or fiber-low groups. We compared the distribution of clinical parameters obtained from medical records between the fiber-high (n\u2009=\u200936) and fiber-low (n\u2009=\u200968) patient groups. We assessed the association between fiber phenotype and enterocolitis using univariate and multivariate logistic regression adjusted for age at operation.<\/jats:p><jats:p>\n          Results\u2003Enterocolitis was more prevalent in patients with sparse mucosal nerve fiber innervation (fiber-low phenotype, 87%) compared with the fiber-high phenotype (13%; p\u2009=\u20090.002). In addition, patients developing enterocolitis had a younger age at surgery (3 vs. 7 months; p\u2009=\u20090.016). In the univariate analysis, the odds for enterocolitis development in the fiber-low phenotype was 5.26 (95% confidence interval [CI], 1.67\u201316.59; p\u2009=\u20090.005) and 4.01 (95% CI, 1.22\u201313.17; p\u2009=\u20090.022) when adjusted for age.<\/jats:p><jats:p>\n          Conclusion\u2003Here, we showed that HSCR patients with a low mucosal nerve fiber innervation grade in the distal aganglionic colon have a higher risk of developing HAEC. Consequently, histopathologic analysis of the nerve fiber innervation grade could serve as a novel sensitive prognostic marker associated with the development of enterocolitis in HSCR patients.<\/jats:p>","DOI":"10.1055\/a-1889-6355","type":"journal-article","created":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T19:23:46Z","timestamp":1656703426000},"page":"299-309","source":"Crossref","is-referenced-by-count":3,"title":["Associations of Mucosal Nerve Fiber Innervation Density with Hirschsprung-Associated Enterocolitis: A Retrospective Three-Center Cohort Study"],"prefix":"10.1055","volume":"33","author":[{"given":"Mich\u00e8le","family":"Moesch","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery, University Children's Hospital Basel, Basel, BS, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jakob","family":"Usemann","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pulmonology, UKBB Ringgold Standard Institution, Basel, BS, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth","family":"Bruder","sequence":"additional","affiliation":[{"name":"Institute of Medical Genetics and Pathology, University Hospital Basel, Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philipp","family":"Romero","sequence":"additional","affiliation":[{"name":"Division of Pediatric Surgery, University of Heidelberg, Heidelberg, Baden-W\u00fcrttemberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Constantin","family":"Schwab","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Hospital Heidelberg Institute of Pathology Ringgold Standard Institution, Heidelberg, Baden-W\u00fcrttemberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beate","family":"Niesler","sequence":"additional","affiliation":[{"name":"Department of Human Molecular Genetics, University Hospital Heidelberg Institute of Human Genetics Ringgold Standard Institution, Heidelberg, Baden-W\u00fcrttemberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Angeles","family":"Tapia-Laliena","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rasul","family":"Khasanov","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tauseef","family":"Nisar","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Holland-Cunz","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery, University Children's Hospital Basel, Basel, BS, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone","family":"Keck","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery, University Children's Hospital Basel, Basel, BS, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Study Group NIG Retro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2022,10,14]]},"reference":[{"issue":"06","key":"ref1","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1016\/j.jpedsurg.2014.01.039","article-title":"Age at presentation of common pediatric surgical conditions: reexamining dogma","volume":"49","author":"J Aboagye","year":"2014","journal-title":"J Pediatr Surg"},{"issue":"03","key":"ref2","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1055\/s-2008-1038625","article-title":"Hirschsprung's disease, one of the most difficult diagnoses in pediatric surgery: a review of the problems from clinical practice to the bench","volume":"18","author":"G Martucciello","year":"2008","journal-title":"Eur J Pediatr Surg"},{"issue":"05","key":"ref3","first-page":"264","article-title":"Diagnosis of Hirschsprung's disease with particular emphasis on histopathology. A systematic review of current literature","volume":"9","author":"L Szylberg","year":"2014","journal-title":"Prz Gastroenterol"},{"issue":"03","key":"ref4","first-page":"568","article-title":"A genetic study of Hirschsprung disease","volume":"46","author":"J A Badner","year":"1990","journal-title":"Am J Hum Genet"},{"issue":"04","key":"ref5","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1097\/01.sla.0000089854.00436.cd","article-title":"One-stage transanal Soave pullthrough for Hirschsprung disease: a multicenter experience with 141 children","volume":"238","author":"J C Langer","year":"2003","journal-title":"Ann Surg"},{"issue":"01","key":"ref6","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/0022-3468(95)90615-0","article-title":"Enterocolitis associated with Hirschsprung's disease: a clinical-radiological characterization based on 168 patients","volume":"30","author":"E A Elhalaby","year":"1995","journal-title":"J Pediatr Surg"},{"issue":"04","key":"ref7","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1053\/j.sempedsurg.2012.07.007","article-title":"Hirschsprung-associated enterocolitis: prevention and therapy","volume":"21","author":"P K Frykman","year":"2012","journal-title":"Semin Pediatr Surg"},{"issue":"09","key":"ref8","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1007\/s00383-013-3353-1","article-title":"Hirschsprung-associated enterocolitis: pathogenesis, treatment and prevention","volume":"29","author":"F R Demehri","year":"2013","journal-title":"Pediatr Surg Int"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/S0140-6736(82)90216-1","article-title":"Association between Clostridium difficile and enterocolitis in Hirschsprung's disease","volume":"1","author":"D FM Thomas","year":"1982","journal-title":"Lancet"},{"issue":"04","key":"ref10","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1080\/19490976.2020.1711685","article-title":"Prospective study reveals a microbiome signature that predicts the occurrence of post-operative enterocolitis in Hirschsprung disease (HSCR) patients","volume":"11","author":"W Tang","year":"2020","journal-title":"Gut Microbes"},{"issue":"04","key":"ref11","doi-asserted-by":"crossref","first-page":"e0124172","DOI":"10.1371\/journal.pone.0124172","article-title":"Characterization of bacterial and fungal microbiome in children with Hirschsprung disease with and without a history of enterocolitis: a multicenter study","volume":"10","author":"P K Frykman","year":"2015","journal-title":"PLoS One"},{"issue":"02","key":"ref12","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.bbrc.2014.01.104","article-title":"Characterization of the intestinal microbiome of Hirschsprung's disease with and without enterocolitis","volume":"445","author":"Z Yan","year":"2014","journal-title":"Biochem Biophys Res Commun"},{"issue":"09","key":"ref13","doi-asserted-by":"crossref","first-page":"e0162079","DOI":"10.1371\/journal.pone.0162079","article-title":"Characterization of intestinal microbiomes of Hirschsprung's disease patients with or without enterocolitis using Illumina-MiSeq high-throughput sequencing","volume":"11","author":"Y Li","year":"2016","journal-title":"PLoS One"},{"issue":"05","key":"ref14","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1002\/mabi.201400473","article-title":"Mucus barriers to microparticles and microbes are altered in Hirschsprung's disease","volume":"15","author":"H M Yildiz","year":"2015","journal-title":"Macromol Biosci"},{"issue":"02","key":"ref15","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/s00383-017-4178-0","article-title":"Altered goblet cell function in Hirschsprung's disease","volume":"34","author":"H Nakamura","year":"2018","journal-title":"Pediatr Surg Int"},{"issue":"03","key":"ref16","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1053\/jpsu.2003.50071","article-title":"MUC-2 mucin production in Hirschsprung's disease: possible association with enterocolitis development","volume":"38","author":"A F Mattar","year":"2003","journal-title":"J Pediatr Surg"},{"issue":"02","key":"ref17","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/j.jcmgh.2021.03.004","article-title":"Lack of mucosal cholinergic innervation is associated with increased risk of enterocolitis in Hirschsprung's disease","volume":"12","author":"S Keck","year":"2021","journal-title":"Cell Mol Gastroenterol Hepatol"},{"issue":"06","key":"ref18","doi-asserted-by":"crossref","first-page":"e0128822","DOI":"10.1371\/journal.pone.0128822","article-title":"Impaired cellular immunity in the murine neural crest conditional deletion of endothelin receptor-B model of Hirschsprung's disease","volume":"10","author":"A Gosain","year":"2015","journal-title":"PLoS One"},{"issue":"03","key":"ref19","doi-asserted-by":"crossref","first-page":"e59066","DOI":"10.1371\/journal.pone.0059066","article-title":"Induction of RET dependent and independent pro-inflammatory programs in human peripheral blood mononuclear cells from Hirschsprung patients","volume":"8","author":"M Rusmini","year":"2013","journal-title":"PLoS One"},{"issue":"02","key":"ref20","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1055\/s-2008-1072336","article-title":"Elevated tissue immunoglobulins in Hirschsprung's disease\u2013indication of early immunologic response","volume":"10","author":"S W Moore","year":"2000","journal-title":"Eur J Pediatr Surg"},{"issue":"02","key":"ref21","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/S0016-5085(53)80003-X","article-title":"Congenital megacolon; a comparison of the spastic and hypertrophied segments with respect to cholinesterase activities and sensitivities to acetylcholine, DFP and the barium ion","volume":"24","author":"K Kamijo","year":"1953","journal-title":"Gastroenterology"},{"issue":"04","key":"ref22","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1007\/s00383-005-1383-z","article-title":"Acetylcholinesterase in Hirschsprung's disease","volume":"21","author":"S W Moore","year":"2005","journal-title":"Pediatr Surg Int"},{"issue":"02","key":"ref23","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/S0016-5085(74)80114-9","article-title":"Functional importance of extrinsic parasympathetic innervation to the distal colon and rectum in man","volume":"66","author":"G Devroede","year":"1974","journal-title":"Gastroenterology"},{"issue":"02","key":"ref24","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1002\/cne.902570209","article-title":"Origin and morphology of nerve fibers in the aganglionic colon of the lethal spotted (ls\/ls) mutant mouse","volume":"257","author":"R F Payette","year":"1987","journal-title":"J Comp Neurol"},{"issue":"03","key":"ref25","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/S0005-8165(72)80058-1","article-title":"The pathophysiology of aganglionosis of the entire colon (Zuelzer-Wilson syndrome). Morphometric investigations of the extent of sacral parasympathetic innervation of the circular muscles of the aganglionic colon","volume":"147","author":"W Meier-Ruge","year":"1972","journal-title":"Beitr Pathol"},{"issue":"01","key":"ref26","doi-asserted-by":"crossref","first-page":"6","DOI":"10.2350\/08-02-0424.1","article-title":"Calretinin immunohistochemistry versus acetylcholinesterase histochemistry in the evaluation of suction rectal biopsies for Hirschsprung disease","volume":"12","author":"R P Kapur","year":"2009","journal-title":"Pediatr Dev Pathol"},{"issue":"02","key":"ref27","first-page":"105","article-title":"Enzyme histochemistry of classical and ultrashort Hirschsprung's disease [in German]","volume":"28","author":"E Bruder","year":"2007","journal-title":"Pathologe"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1016\/S0140-6736(69)90144-5","article-title":"Histochemical diagnosis of Hirschsprung's disease","volume":"2","author":"J R Garrett","year":"1969","journal-title":"Lancet"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1136\/bmj.3.5672.718-c","article-title":"Bowel innervation in Hirschsprung's disease","volume":"3","author":"J R Garrett","year":"1969","journal-title":"BMJ"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1038\/35013070","article-title":"Vagus nerve stimulation attenuates the systemic inflammatory response to endotoxin","volume":"405","author":"L V Borovikova","year":"2000","journal-title":"Nature"},{"issue":"04","key":"ref31","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1053\/j.gastro.2006.08.016","article-title":"The vagus nerve: a tonic inhibitory influence associated with inflammatory bowel disease in a murine model","volume":"131","author":"J E Ghia","year":"2006","journal-title":"Gastroenterology"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"464","DOI":"10.2119\/molmed.2016.00062","article-title":"Vagotomy affects the development of oral tolerance and increases susceptibility to develop colitis independently of the alpha-7 nicotinic receptor","volume":"22","author":"M Di Giovangiulio","year":"2016","journal-title":"Mol Med"},{"issue":"06","key":"ref33","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1136\/gutjnl-2013-304676","article-title":"A distinct vagal anti-inflammatory pathway modulates intestinal muscularis resident macrophages independent of the spleen","volume":"63","author":"G Matteoli","year":"2014","journal-title":"Gut"},{"issue":"22","key":"ref34","doi-asserted-by":"crossref","first-page":"2740","DOI":"10.3748\/wjg.v17.i22.2740","article-title":"Ulcerative colitis in smokers, non-smokers and ex-smokers","volume":"17","author":"G Bastida","year":"2011","journal-title":"World J Gastroenterol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"418","DOI":"10.3389\/fnins.2019.00418","article-title":"Anti-inflammatory effects of abdominal vagus nerve stimulation on experimental intestinal inflammation","volume":"13","author":"S C Payne","year":"2019","journal-title":"Front Neurosci"},{"issue":"10","key":"ref36","doi-asserted-by":"crossref","first-page":"e13911","DOI":"10.1111\/nmo.13911","article-title":"A 12-month pilot study outcomes of vagus nerve stimulation in Crohn's disease","volume":"32","author":"V Sinniger","year":"2020","journal-title":"Neurogastroenterol Motil"},{"issue":"02","key":"ref37","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/j.jcmgh.2021.05.012","article-title":"Nerves make the bowel happy, even when the enteric nervous system is missing!","volume":"12","author":"R O Heuckeroth","year":"2021","journal-title":"Cell Mol Gastroenterol Hepatol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"781147","DOI":"10.3389\/fimmu.2021.781147","article-title":"Cholinergic signaling attenuates pro-inflammatory interleukin-8 response in colonic epithelial cells","volume":"12","author":"I M\u00fcller","year":"2022","journal-title":"Front Immunol"},{"issue":"03","key":"ref39","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1111\/nmo.12738","article-title":"Bristol Stool Form Scale reliability and agreement decreases when determining Rome III stool form designations","volume":"28","author":"B P Chumpitazi","year":"2016","journal-title":"Neurogastroenterol Motil"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"7B","DOI":"10.1155\/2011\/548024","article-title":"What is chronic constipation? Definition and diagnosis","volume":"25","author":"J R Gray","year":"2011","journal-title":"Can J Gastroenterol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"x","DOI":"10.1186\/s43159-019-0003-y","article-title":"Obstructive complications after pull-through for Hirschsprung's disease: different causes and tailored management","volume":"15","author":"M Elsherbeny","year":"2019","journal-title":"Ann Pediatr Surg"},{"issue":"01","key":"ref42","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.jpedsurg.2008.10.053","article-title":"Hirschsprung disease and fecal incontinence: diagnostic and management strategies","volume":"44","author":"M A Levitt","year":"2009","journal-title":"J Pediatr Surg"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"6483","DOI":"10.1016\/j.vaccine.2017.01.051","article-title":"Failure to thrive: case definition & guidelines for data collection, analysis, and presentation of maternal immunisation safety data","volume":"35","author":"E Ross","year":"2017","journal-title":"Vaccine"},{"issue":"06","key":"ref44","doi-asserted-by":"crossref","first-page":"F551","DOI":"10.1136\/archdischild-2016-311765","article-title":"Epidemiology, management and outcome of ultrashort bowel syndrome in infancy","volume":"102","author":"A Batra","year":"2017","journal-title":"Arch Dis Child Fetal Neonatal Ed"},{"issue":"01","key":"ref45","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.jpedsurg.2008.10.052","article-title":"Development of a standardized definition for Hirschsprung's-associated enterocolitis: a Delphi analysis","volume":"44","author":"A C Pastor","year":"2009","journal-title":"J Pediatr Surg"},{"key":"ref46","first-page":"1","article-title":"Pathology of chronic constipation in pediatric and adult coloproctology","volume":"72","author":"W A Meier-Ruge","year":"2005","journal-title":"Pathobiology"},{"issue":"02","key":"ref47","first-page":"212","article-title":"Resection of rectum and rectosigmoid with preservation of the sphincter for benign spastic lesions producing megacolon; an experimental study","volume":"24","author":"O Swenson","year":"1948","journal-title":"Surgery"},{"issue":"01","key":"ref48","first-page":"92","article-title":"Hirschsprung's disease: Rehbein's intra-abdominal resection technic [in French]","volume":"11","author":"F Rehbein","year":"1970","journal-title":"Ann Chir Infant"},{"key":"ref49","first-page":"2249","article-title":"New operation for congenital megacolon: retrorectal and transanal lowering of the colon, and its possible application to the treatment of various other malformations [in French]","volume":"64","author":"B Duhamel","year":"1956","journal-title":"Presse Med"},{"key":"ref50","first-page":"1007","article-title":"A new surgical technique for treatment of Hirschsprung's disease","volume":"56","author":"F Soave","year":"1964","journal-title":"Surgery"},{"issue":"08","key":"ref51","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1016\/S0022-3468(98)90169-5","article-title":"Transanal endorectal pull-through for Hirschsprung's disease","volume":"33","author":"L De la Torre-Mondrag\u00f3n","year":"1998","journal-title":"J Pediatr Surg"},{"issue":"05","key":"ref52","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1097\/00000658-199905000-00010","article-title":"Primary laparoscopic-assisted endorectal colon pull-through for Hirschsprung's disease: a new gold standard","volume":"229","author":"K E Georgeson","year":"1999","journal-title":"Ann Surg"},{"issue":"05","key":"ref53","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/0022-3468(77)90392-X","article-title":"Histochemical criteria for the diagnosis of Hirschsprung's disease in rectal suction biopsies by acetylcholinesterase activity","volume":"12","author":"C W Chow","year":"1977","journal-title":"J Pediatr Surg"},{"issue":"02","key":"ref54","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1097\/00005176-199008000-00012","article-title":"Acetylcholinesterase-stained suction rectal biopsies in the diagnosis of Hirschsprung's disease","volume":"11","author":"D E Schofield","year":"1990","journal-title":"J Pediatr Gastroenterol Nutr"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1038\/s41586-020-2474-7","article-title":"Microbiota modulate sympathetic neurons via a gut-brain circuit","volume":"583","author":"P A Muller","year":"2020","journal-title":"Nature"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/s40168-016-0156-3","article-title":"Bloom and bust: intestinal microbiota dynamics in response to hospital exposures and Clostridium difficile colonization or infection","volume":"4","author":"C Vincent","year":"2016","journal-title":"Microbiome"},{"issue":"07","key":"ref57","doi-asserted-by":"crossref","first-page":"e1002517","DOI":"10.1371\/journal.pbio.1002517","article-title":"Host gut motility promotes competitive exclusion within a model intestinal microbiota","volume":"14","author":"T J Wiles","year":"2016","journal-title":"PLoS Biol"},{"issue":"03","key":"ref58","doi-asserted-by":"crossref","first-page":"G368","DOI":"10.1152\/ajpgi.2001.280.3.G368","article-title":"Influence of microbial species on small intestinal myoelectric activity and transit in germ-free rats","volume":"280","author":"E Husebye","year":"2001","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"issue":"02","key":"ref59","doi-asserted-by":"crossref","first-page":"e2000689","DOI":"10.1371\/journal.pbio.2000689","article-title":"The enteric nervous system promotes intestinal health by constraining microbiota composition","volume":"15","author":"A S Rolig","year":"2017","journal-title":"PLoS Biol"},{"issue":"11","key":"ref60","doi-asserted-by":"crossref","first-page":"e1009106","DOI":"10.1371\/journal.pgen.1009106","article-title":"A complementary study approach unravels novel players in the pathoetiology of Hirschsprung disease","volume":"16","author":"T Mederer","year":"2020","journal-title":"PLoS Genet"},{"issue":"01","key":"ref61","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1136\/jmg.2007.053959","article-title":"Hirschsprung disease, associated syndromes and genetics: a review","volume":"45","author":"J Amiel","year":"2008","journal-title":"J Med Genet"},{"issue":"15","key":"ref62","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1056\/NEJMoa1706594","article-title":"Molecular genetic anatomy and risk profile of Hirschsprung's disease","volume":"380","author":"J M Tilghman","year":"2019","journal-title":"N Engl J Med"},{"issue":"06","key":"ref63","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1016\/j.jpedsurg.2008.02.039","article-title":"Older age at diagnosis of Hirschsprung disease decreases risk of postoperative enterocolitis, but resection of additional ganglionated bowel does not","volume":"43","author":"R N Haricharan","year":"2008","journal-title":"J Pediatr Surg"},{"issue":"05","key":"ref64","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1055\/s-0033-1333635","article-title":"Are routine dilatations necessary post pull-through surgery for Hirschsprung disease?","volume":"23","author":"O Aworanti","year":"2013","journal-title":"Eur J Pediatr Surg"},{"issue":"02","key":"ref65","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1097\/MPG.0000000000002358","article-title":"Inflammatory bowel disease serological immune markers anti-saccharomyces cerevisiae mannan antibodies and outer membrane porin C are potential biomarkers for Hirschsprung-associated enterocolitis","volume":"69","author":"P K Frykman","year":"2019","journal-title":"J Pediatr Gastroenterol Nutr"},{"issue":"01","key":"ref66","doi-asserted-by":"crossref","first-page":"21725","DOI":"10.1038\/s41598-020-78340-z","article-title":"Analysis of enteric nervous system and intestinal epithelial barrier to predict complications in Hirschsprung's disease","volume":"10","author":"A Dariel","year":"2020","journal-title":"Sci Rep"},{"issue":"01","key":"ref67","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.jpedsurg.2012.10.028","article-title":"Probiotic prophylaxis after pullthrough for Hirschsprung disease to reduce incidence of enterocolitis: a prospective, randomized, double-blind, placebo-controlled, multicenter trial","volume":"48","author":"M El-Sawaf","year":"2013","journal-title":"J Pediatr Surg"},{"issue":"04","key":"ref68","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1056\/NEJMcpc1103562","article-title":"Case records of the Massachusetts General Hospital. Case 3-2012. 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