{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T17:08:42Z","timestamp":1783098522189,"version":"3.54.6"},"reference-count":40,"publisher":"Georg Thieme Verlag KG","issue":"03","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ferroptosis, as a kind of non-apoptotic cell death, is involved in the pathogenesis of type 1 diabetes mellitus (T1DM). Islet B cells mainly produce insulin that is used to treat diabetes. Berberine (BBR) can ameliorate type 2 diabetes and insulin resistance in many ways. However, a few clues concerning the mechanism of BBR regulating ferroptosis of islet \u03b2 cells in T1DM have been detected so far. We measured the effects of BBR and GPX4 on islet \u03b2 cell viability and proliferation by MTT and colony formation assays. Western blot and qRT-PCR were utilized to examine GPX4 expression in islet \u03b2 cells with distinct treatments. The influence of BBR and GPX4 on ferroptosis of islet \u03b2 cells was investigated by evaluating the content of Fe2+ and reactive oxygen species (ROS) in cells. The mechanism of BBR targeting GPX4 to inhibit ferroptosis of islet \u03b2 cells was further revealed by the rescue experiment. Our results showed that BBR and overexpression of GPX4 could notably accelerate cell viability and the proliferative abilities of islet \u03b2\u00a0cells. Moreover, BBR stimulated GPX4 expression to reduce the content of Fe2+ and ROS, thereby repressing the ferroptosis of islet \u03b2 cells, which functioned similarly as ferroptosis inhibitor Fer-1. In conclusion, BBR suppressed ferroptosis of islet \u03b2 cells via promoting GPX4 expression, providing new insights into the mechanism of BBR for islet \u03b2 cells.<\/jats:p>","DOI":"10.1055\/a-1939-7417","type":"journal-article","created":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T23:45:30Z","timestamp":1668037530000},"page":"254-261","source":"Crossref","is-referenced-by-count":25,"title":["Berberine Regulates GPX4 to Inhibit Ferroptosis of Islet \u03b2 Cells"],"prefix":"10.1055","volume":"89","author":[{"given":"Lei","family":"Bao","sequence":"first","affiliation":[{"name":"Department of Endocrinology, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu, Jiangsu Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixuan","family":"Jin","sequence":"first","affiliation":[{"name":"Department of Endocrinology, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu, Jiangsu Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiani","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu, Jiangsu Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanqiu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu, Jiangsu Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingling","family":"Qian","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu, Jiangsu Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1987-3827","authenticated-orcid":false,"given":"Weiming","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu, Jiangsu Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2022,11,9]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.diabres.2017.03.024","article-title":"IDF Diabetes Atlas: Global estimates for the prevalence of diabetes for 2015 and 2040","volume":"128","author":"K Ogurtsova","year":"2017","journal-title":"Diabetes Res Clin Pract"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.diabres.2013.11.002","article-title":"Global estimates of diabetes prevalence for 2013 and projections for 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