{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T13:00:56Z","timestamp":1777122056380,"version":"3.51.4"},"reference-count":36,"publisher":"Georg Thieme Verlag KG","issue":"03","funder":[{"name":"This research was funded by grants from the Hunan Provincial\n                    Health Commission","award":["No. 20201906"],"award-info":[{"award-number":["No. 20201906"]}]},{"name":"the Hunan Provincial\n                        Health and Family Planning Commission","award":["A2017018"],"award-info":[{"award-number":["A2017018"]}]},{"name":"Hunan Provincial Clinical Medical Research Center","award":["2021SK4030"],"award-info":[{"award-number":["2021SK4030"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Exp Clin Endocrinol Diabetes"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Objective The E2F2 transcription factor can accelerate cell proliferation\n                    and wound healing. However, its mechanism of action in a diabetic foot ulcer\n                    (DFU) remains unclear. Therefore, this study explores the influence of E2F2 on\n                    wound healing in DFU by examining cell division cycle-associated 7-like (CDCA7L)\n                    expression.<\/jats:p><jats:p>\n          Methods CDCA7L and E2F2 expression in DFU tissues were analyzed with\n                    databases. CDCA7L and E2F2 expression were altered in human umbilical vein\n                    endothelial cells (HUVECs) and spontaneously transformed human keratinocyte cell\n                    culture (HaCaT) cells. Cell viability, migration, colony formation, and\n                    angiogenesis were evaluated. Binding of E2F2 to the CDCA7L promoter was\n                    examined. Subsequently, a diabetes mellitus (DM) mouse model was established and\n                    treated with full-thickness excision followed by CDCA7L overexpression. Wound\n                    healing in these mice was observed and recorded, and vascular endothelial growth\n                    factor receptor 2 (VEGFR2) and hematopoietic progenitor cell antigen CD34 (CD34)\n                    expression were determined. E2F2 and CDCA7L expression levels in cells and mice\n                    were evaluated. The expression of growth factors was tested.<\/jats:p><jats:p>\n          Results CDCA7L expression was downregulated in DFU tissues and wound\n                    tissues from DM mice. Mechanistically, E2F2 bound to the CDCA7L promoter to\n                    upregulate CDCA7L expression. E2F2 overexpression enhanced viability, migration,\n                    and growth factor expression in HaCaT cells and HUVECs, and augmented HUVEC\n                    angiogenesis and HaCaT cell proliferation, which was nullified by silencing\n                    CDCA7L. In DM mice, CDCA7L overexpression facilitated wound healing and elevated\n                    the expression level of growth factors.<\/jats:p><jats:p>\n          Conclusions E2F2 facilitated cell proliferation and migration and fostered\n                    wound healing in DFU cells through binding to the CDCA7L promoter.<\/jats:p>","DOI":"10.1055\/a-1989-1918","type":"journal-article","created":{"date-parts":[[2023,3,10]],"date-time":"2023-03-10T00:02:26Z","timestamp":1678406546000},"page":"162-172","source":"Crossref","is-referenced-by-count":6,"title":["E2F2 Promotes Wound Healing of Diabetic Foot Ulcer by Regulating\n                    CDCA7L Transcription"],"prefix":"10.1055","volume":"131","author":[{"given":"Meimei","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Hand and Foot Microsurgery, The Affiliated Nanhua\n                    Hospital, Hengyang Medical College, University of South China, Hengyang, Hunan,\n                    P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiusong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hand and Foot Microsurgery, The Affiliated Nanhua\n                    Hospital, Hengyang Medical College, University of South China, Hengyang, Hunan,\n                    P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanming","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Hand and Foot Microsurgery, The Affiliated Nanhua\n                    Hospital, Hengyang Medical College, University of South China, Hengyang, Hunan,\n                    P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2023,3,9]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"107843","DOI":"10.1016\/j.diabres.2019.107843","article-title":"Global and regional diabetes prevalence estimates for 2019 and projections for\n                    2030 and 2045: Results from the International Diabetes Federation Diabetes\n                    Atlas, 9(th) edition","volume":"157","author":"P Saeedi","year":"2019","journal-title":"Diabetes Res Clin Pract"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"108615","DOI":"10.1016\/j.biopha.2019.108615","article-title":"Mechanistic insight into diabetic wounds: Pathogenesis, molecular targets and\n                    treatment strategies to pace wound healing","volume":"112","author":"S Patel","year":"2019","journal-title":"Biomed 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Zhao","year":"2018","journal-title":"Oncogene"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21124473","article-title":"Inactivation of APC Induces CD34 Upregulation to Promote Epithelial-Mesenchymal\n                    Transition and Cancer Stem Cell Traits in Pancreatic Cancer","volume":"21","author":"M J Hsieh","year":"2020","journal-title":"Int J Mol Sci"},{"key":"ref36","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1186\/s13046-019-1156-5","article-title":"ACE2 inhibits breast cancer angiogenesis via suppressing the\n                    VEGFa\/VEGFR2\/ERK pathway","volume":"38","author":"Q Zhang","year":"2019","journal-title":"J Exp Clin Cancer Res"}],"container-title":["Experimental and Clinical Endocrinology &amp; Diabetes"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-1989-1918.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,10]],"date-time":"2023-03-10T00:03:15Z","timestamp":1678406595000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-1989-1918"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3]]},"references-count":36,"journal-issue":{"issue":"03","published-online":{"date-parts":[[2023,3,9]]},"published-print":{"date-parts":[[2023,3]]}},"URL":"https:\/\/doi.org\/10.1055\/a-1989-1918","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["0947-7349","1439-3646"],"issn-type":[{"value":"0947-7349","type":"print"},{"value":"1439-3646","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3]]}}}