{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T13:32:45Z","timestamp":1776087165566,"version":"3.50.1"},"reference-count":39,"publisher":"Georg Thieme Verlag KG","issue":"07","funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","award":["310755\/2017-4"],"award-info":[{"award-number":["310755\/2017-4"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","award":["465425\/2014-3"],"award-info":[{"award-number":["465425\/2014-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gouty arthritis (GA) is an inflammatory arthritis triggered by the deposition of monosodium urate monohydrate (MSU) crystals, causing pain, inflammation, and joint damage. Several drugs are currently employed to manage acute flares of GA, but they either have limited effectiveness or induce severe adverse reactions. Ouratea spectabilis is traditionally used in Brazil to treat gastric ulcers and rheumatism. The ethanolic extract of O. spectabilis stems (OSpC) and four biflavanones (ouratein A\u200a\u2013\u200aD) isolated thereof were evaluated in a murine model of GA induced by the injection of MSU crystals. The underlying mechanism of action of ouratein D was investigated in vitro in cell cultures by measurement of IL-1\u03b2 levels by ELISA and Western blot analysis. The administration of OSpC (10, 30 or 100\u2009mg\/Kg, p.\u200ao.) reduced the migration of total inflammatory cells, monocytes, and neutrophils and diminished the levels of IL-1\u03b2 and CXCL1 in the synovial tissue. Among the tested compounds, only ouratein D (1\u2009mg\/Kg) reduced the migration of the inflammatory cells and it was shown to be active up to 0.01\u2009mg\/Kg (equivalent to 0.34\u2009nM\/Kg, p.\u200ao.). Treatment of pre-stimulated THP-1 cells (differentiated into macrophages) or BMDMs with ouratein D reduced the release of IL-1\u03b2 in both macrophage lines. This biflavanone reduced the activation of caspase-1 (showed by the increase in the cleaved form) in supernatants of cultured BMDMs, evidencing its action in modulating the inflammasome pathway. The obtained results demonstrate the anti-gout properties of O. spectabilis and point out ouratein D as the bioactive component of the assayed extract.<\/jats:p>","DOI":"10.1055\/a-2009-9809","type":"journal-article","created":{"date-parts":[[2023,1,11]],"date-time":"2023-01-11T00:05:57Z","timestamp":1673395557000},"page":"718-728","source":"Crossref","is-referenced-by-count":8,"title":["Ouratea spectabilis and its Biflavanone Ouratein D Exert Potent Anti-inflammatory Activity in MSU Crystal-induced Gout in Mice"],"prefix":"10.1055","volume":"89","author":[{"given":"Marina P.","family":"Rocha","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego P.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vivian L. S.","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabella","family":"Zaidan","sequence":"additional","affiliation":[{"name":"Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"La\u00eds C.","family":"Grossi","sequence":"additional","affiliation":[{"name":"Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5809-9037","authenticated-orcid":false,"given":"Priscilla R. V.","family":"Campana","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1vio A.","family":"Amaral","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lirl\u00e2ndia P.","family":"Sousa","sequence":"additional","affiliation":[{"name":"Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro M.","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9468-376X","authenticated-orcid":false,"given":"Fern\u00e3o C.","family":"Braga","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2023,3,15]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1038\/s41584-020-0441-1","article-title":"Global epidemiology of gout: Prevalence, incidence, treatment patterns and risk factors","volume":"16","author":"M Dehlin","year":"2020","journal-title":"Nat Rev Rheumatol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"S11","DOI":"10.1016\/j.semarthrit.2020.04.008","article-title":"Gout epidemiology and comorbidities","volume":"50","author":"J A Singh","year":"2020","journal-title":"Semin Arthritis Rheum"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1038\/s41572-019-0115-y","article-title":"Gout","volume":"5","author":"N Dalbeth","year":"2019","journal-title":"Nat Rev Dis Primers"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11926-013-0400-9","article-title":"The crystallization of monosodium urate","volume":"16","author":"M A Martillo","year":"2014","journal-title":"Curr Rheumatol Rep"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1038\/s41584-020-0455-8","article-title":"Cell death in chronic inflammation: breaking the cycle to treat rheumatic disease","volume":"16","author":"H Anderton","year":"2020","journal-title":"Nat Rev Rheumatol"},{"key":"ref6","first-page":"39","article-title":"The role of the NLRP3 inflammasome in gout","volume":"4","author":"S R Kingsbury","year":"2011","journal-title":"J Inflamm Res"},{"key":"ref7","first-page":"6193","article-title":"Peptic ulcer hemorrhage combined with acute gout: Analyses of treatment in 136 cases","volume":"8","author":"Z Xu","year":"2015","journal-title":"Int J Clin Exp Med"},{"key":"ref8","first-page":"346","article-title":"Critical revision of the medical treatment of gout in Brazil","volume":"57","author":"V F Azevedo","year":"2017","journal-title":"Rev Bras Reumatol Engl Ed"},{"key":"ref9","first-page":"324","article-title":"Essence of the revised guideline for the management of hyperuricemia and gout","volume":"55","author":"H TG Yamanaka","year":"2012","journal-title":"Japan Med Assoc J"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"S24","DOI":"10.1016\/j.semarthrit.2020.04.010","article-title":"Therapeutic approaches in the treatment of gout","volume":"50","author":"M H Pillinger","year":"2020","journal-title":"Semin Arthritis Rheum"},{"key":"ref11","first-page":"8637583","article-title":"Medicinal plants as a drug alternative source for the antigout therapy in Morocco","volume":"2020","author":"N E Daoudi","year":"2020","journal-title":"Scientifica (Cairo)"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"251","DOI":"10.3390\/medicines2030251","article-title":"Modes of action of herbal medicines and plant secondary metabolites","volume":"2","author":"M Wink","year":"2015","journal-title":"Medicines (Basel)"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"397","DOI":"10.3417\/2006096","article-title":"A distinctive new species of Ouratea (Ochnaceae) from Jalap\u00e3o Region, Tocantins, Brazil","volume":"18","author":"RG Chacon","year":"2008","journal-title":"Novon"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.sajb.2014.10.002","article-title":"Phytotoxicity of extracts and fractions of Ouratea spectabilis (Mart. 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