{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:52:32Z","timestamp":1792785152097,"version":"4.5.0"},"reference-count":24,"publisher":"Georg Thieme Verlag KG","issue":"05","license":[{"start":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T00:00:00Z","timestamp":1676246400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Enhanced perioperative oncology (EPEONC) consortium"},{"DOI":"10.13039\/100008363","name":"Kr\u00e6ftens Bek\u00e6mpelse","doi-asserted-by":"crossref","award":["R110-A6996"],"award-info":[{"award-number":["R110-A6996"]}],"id":[{"id":"10.13039\/100008363","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Endosc Int Open"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Background and study aims\u2002Colorectal cancer is one of the most common malignancies, with approximately 20\u200a% of patients having metastatic disease. Local symptoms from the tumor remain a common issue and affect quality of life. Electroporation is a method to permeabilize cell membranes with high-voltage pulses, allowing increased passage of otherwise poorly permeating substances such as calcium. The aim of this study was to determine the safety of calcium electroporation for advanced colorectal cancer.<\/jats:p>\n                  <jats:p>Patients and methods\u2002Six patients with inoperable rectal and sigmoid colon cancer were included, all presenting with local symptoms. Patients were offered endoscopic calcium electroporation and were followed up with endoscopy and computed tomography\/magnetic resonance scans. Biopsies and blood samples were collected at baseline and at follow-up, 4, 8, and 12 weeks after treatment. Biopsies were examined for histological changes and immunohistochemically with CD3\/CD8 and PD-L1. In addition, blood samples were examined for circulating cell-free DNA (cfDNA).<\/jats:p>\n                  <jats:p>Results\u2002A total of 10 procedures were performed and no serious adverse events occurred. Prior to inclusion, patients reported local symptoms, such as bleeding (N\u200a=\u200a3), pain (N\u200a=\u200a2), and stenosis (N\u200a=\u200a5). Five of six patients reported symptom relief. In one patient, also receiving systemic chemotherapy, clinical complete response of primary tumor was seen. Immunohistochemistry found no significant changes in CD3\u200a\/CD8 levels or cfDNA levels after treatment.<\/jats:p>\n                  <jats:p>Conclusions\u2002This first study of calcium electroporation for colorectal tumors shows that calcium electroporation is a safe and feasible treatment modality for colorectal cancer. It can be performed as an outpatient treatment and may potentially be of great value for fragile patients with limited treatment options.<\/jats:p>","DOI":"10.1055\/a-2033-9831","type":"journal-article","created":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T19:24:17Z","timestamp":1676316257000},"page":"E451-E459","source":"Crossref","is-referenced-by-count":19,"title":["Endoscopic calcium electroporation for colorectal cancer: a phase I study"],"prefix":"10.1055","volume":"11","clinical-trial-number":[{"clinical-trial-number":"nct03542214","registry":"10.18810\/clinical-trials-gov"}],"author":[{"given":"Malene","family":"Broholm","sequence":"additional","affiliation":[{"name":"Center for Surgical Science, Department of Surgery, Zealand University Hospital, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasmus","family":"Vogelsang","sequence":"additional","affiliation":[{"name":"Center for Surgical Science, Department of Surgery, Zealand University Hospital, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa","family":"Bulut","sequence":"additional","affiliation":[{"name":"Center for Surgical Science, Department of Surgery, Zealand University Hospital, Denmark"},{"name":"Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trine","family":"Stigaard","sequence":"additional","affiliation":[{"name":"Center for Surgical Science, Department of Surgery, Zealand University Hospital, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanne","family":"Falk","sequence":"additional","affiliation":[{"name":"Department of Oncology, Herlev and Gentofte Hospital, University of Copenhagen, Herlev, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stine","family":"Frandsen","sequence":"additional","affiliation":[{"name":"Center for Experimental Drug and Gene Electrotransfer (C*EDGE), Department of Clinical Oncology and Palliative Care, Zealand University Hospital, Roskilde, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorte Levin","family":"Pedersen","sequence":"additional","affiliation":[{"name":"Department of Radiology, Zealand University Hospital, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trine","family":"Perner","sequence":"additional","affiliation":[{"name":"Department of Radiology, Zealand University Hospital, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Marie Kanstrup","family":"Fiehn","sequence":"additional","affiliation":[{"name":"Center for Surgical Science, Department of Surgery, Zealand University Hospital, Denmark"},{"name":"Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark"},{"name":"Department of Pathology, Zealand University Hospital, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ida","family":"M\u00f8lholm","sequence":"additional","affiliation":[{"name":"Visiopharm, Hoersholm, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Bzorek","sequence":"additional","affiliation":[{"name":"Department of Pathology, Zealand University Hospital, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas Weinberger","family":"Rosen","sequence":"additional","affiliation":[{"name":"Center for Surgical Science, Department of Surgery, Zealand University Hospital, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina S\u00f8s Au\u00f0ur","family":"Andersen","sequence":"additional","affiliation":[{"name":"Department of Pathology, Zealand University Hospital, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niels","family":"Pallisgaard","sequence":"additional","affiliation":[{"name":"Department of Pathology, Zealand University Hospital, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ismail","family":"G\u00f6genur","sequence":"additional","affiliation":[{"name":"Center for Surgical Science, Department of Surgery, Zealand University Hospital, Denmark"},{"name":"Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Gehl","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark"},{"name":"Department of Oncology, Herlev and Gentofte Hospital, University of Copenhagen, Herlev, Denmark"},{"name":"Center for Experimental Drug and Gene Electrotransfer (C*EDGE), Department of Clinical Oncology and Palliative Care, Zealand University Hospital, Roskilde, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2023,5,9]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1046\/j.1365-201X.2003.01093.x","article-title":"Electroporation: theory and methods, perspectives for drug delivery, gene therapy and research","volume":"177","author":"J Gehl","year":"2003","journal-title":"Acta Physiol Scand"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"290","DOI":"10.3390\/cancers12020290","article-title":"A comprehensive review of calcium electroporation - a novel cancer treatment modality","volume":"12","author":"S K Frandsen","year":"2020","journal-title":"Cancers"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1336","DOI":"10.1158\/0008-5472.CAN-11-3782","article-title":"Direct therapeutic applications of calcium 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