{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T09:24:46Z","timestamp":1768296286076,"version":"3.49.0"},"reference-count":57,"publisher":"Georg Thieme Verlag KG","issue":"08","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Klin Monbl Augenheilkd"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Purpose To investigate the effect of Rose Bengal photodynamic therapy (RB-PDT) on viability and proliferation of human limbal epithelial stem cells (T-LSCs), human corneal epithelial cells (HCE-T), human limbal fibroblasts (LFCs), and human normal and keratoconus fibroblasts (HCFs and KC-HCFs) in vitro.<\/jats:p><jats:p>\n          Methods T-LSCs and HCE-T cell lines were used in this research. LFCs were isolated from healthy donor corneal limbi (n\u2009=\u20095), HCFs from healthy human donor corneas (n\u2009=\u20095), and KC-HCFs from penetrating keratoplasties of keratoconus patients (n\u2009=\u20095). After cell culture, RB-PDT was performed using 0.001% RB concentration and 565\u2009nm wavelength illumination with 0.14 to 0.7\u2009J\/cm2 fluence. The XTT and the BrdU assays were used to assess cell viability and proliferation 24\u2009h after RB-PDT.<\/jats:p><jats:p>\n          Results RB or illumination alone did not change cell viability or proliferation in any of the cell types (p\u2009\u2265\u20090.1). However, following RB-PDT, viability decreased significantly from 0.17\u2009J\/cm2 fluence in HCFs (p\u2009&lt;\u20090.001) and KC-HCFs (p\u2009&lt;\u20090.0001), and from 0.35\u2009J\/cm2 fluence in T-LSCs (p\u2009&lt;\u20090.001), HCE-T (p\u2009&lt;\u20090.05), and LFCs ((p\u2009&lt;\u20090.0001). Cell proliferation decreased significantly from 0.14\u2009J\/cm2 fluence in T-LSCs (p\u2009&lt;\u20090.0001), HCE-T (p\u2009&lt;\u20090.05), and KC-HCFs (p\u2009&lt;\u20090.001) and from 0.17\u2009J\/cm2 fluence in HCFs (p\u2009&lt;\u20090.05). Regarding LFCs proliferation, no values could be determined by the BrdU assay.<\/jats:p><jats:p>\n          Conclusions Though RB-PDT seems to be a safe and effective treatment method in vivo, its dose-dependent phototoxicity on corneal epithelial and stromal cells has to be respected. The data and experimental parameters applied in this study may provide a reliable reference for future investigations.<\/jats:p>","DOI":"10.1055\/a-2038-8899","type":"journal-article","created":{"date-parts":[[2023,2,21]],"date-time":"2023-02-21T00:20:26Z","timestamp":1676938826000},"page":"972-981","source":"Crossref","is-referenced-by-count":6,"title":["Assessment of Rose Bengal Photodynamic Therapy on Viability and Proliferation of Human Keratolimbal Epithelial and Stromal Cells In Vitro"],"prefix":"10.1055","volume":"241","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9689-8887","authenticated-orcid":false,"given":"Ning","family":"Chai","sequence":"additional","affiliation":[{"name":"Dr. Rolf M. Schwiete Center for Limbal Stem Cell and Aniridia Research, Saarland University, Homburg\/Saar, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanja","family":"Stachon","sequence":"additional","affiliation":[{"name":"Dr. Rolf M. Schwiete Center for Limbal Stem Cell and Aniridia Research, Saarland University, Homburg\/Saar, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahsa","family":"Nastaranpour","sequence":"additional","affiliation":[{"name":"Dr. Rolf M. Schwiete Center for Limbal Stem Cell and Aniridia Research, Saarland University, Homburg\/Saar, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5491-2169","authenticated-orcid":false,"given":"Zhen","family":"Li","sequence":"additional","affiliation":[{"name":"Dr. Rolf M. Schwiete Center for Limbal Stem Cell and Aniridia Research, Saarland University, Homburg\/Saar, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9701-8204","authenticated-orcid":false,"given":"Berthold","family":"Seitz","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Saarland University Hospital and Saarland University, Faculty of Medicine, Homburg\/Saar, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myriam","family":"Ulrich","sequence":"additional","affiliation":[{"name":"Dr. Rolf M. 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Schwiete Center for Limbal Stem Cell and Aniridia Research, Saarland University, Homburg\/Saar, Germany"},{"name":"Department of Ophthalmology, Semmelweis University, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2023,6,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"4152","DOI":"10.1167\/iovs.11-9268","article-title":"Variation in the lysyl oxidase (LOX) gene is associated with keratoconus in family-based and case-control studies","volume":"53","author":"Y Bykhovskaya","year":"2012","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pone.0176017","article-title":"Acute hypoxia influences collagen and matrix metalloproteinase expression by human keratoconus cells in vitro","volume":"12","author":"T B McKay","year":"2017","journal-title":"PLoS 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