{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T15:18:23Z","timestamp":1785251903841,"version":"3.55.0"},"reference-count":48,"publisher":"Georg Thieme Verlag KG","issue":"10","funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","award":["310183\/2020-0"],"award-info":[{"award-number":["310183\/2020-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","award":["420454\/2016-0"],"award-info":[{"award-number":["420454\/2016-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rauvolfia species are well known as producers of bioactive monoterpene indole alkaloids, which exhibit a broad spectrum of biological activities. A new vobasine-sarpagan-type bisindole alkaloid (1) along with six known monomeric indoles (2, 3\/4, 5, and 6\/7) were isolated from the ethanol extract of the roots of Rauvolfia ligustrina. The structure of the new compound was elucidated by interpretation of their spectroscopic data (1D and 2D\u2009NMR and HRESIMS) and comparison with published data for analog compounds. The cytotoxicity of the isolated compounds was screened in a zebrafish (Danio rerio) model. The possible GABAergic (diazepam as the positive control) and serotoninergic (fluoxetine as the positive control) mechanisms of action in adult zebrafish were also evaluated. No compounds were cytotoxic. Compound 2 and the epimers 3\/4 and 6\/7 showed a mechanism action by GABAA, while compound 1 showed a mechanism action by a serotonin receptor (anxiolytic activity). Molecular docking studies showed that compounds 2 and 5 have a greater affinity by the GABAA receptor when compared with diazepam, whereas 1 showed the best affinity for the 5HT2AR channel when compared to risperidone.<\/jats:p>","DOI":"10.1055\/a-2058-3710","type":"journal-article","created":{"date-parts":[[2023,4,4]],"date-time":"2023-04-04T13:31:48Z","timestamp":1680615108000},"page":"979-989","source":"Crossref","is-referenced-by-count":5,"title":["Indole Alkaloids of Rauvolfia ligustrina and Their Anxiolytic Effects in Adult Zebrafish"],"prefix":"10.1055","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0995-783X","authenticated-orcid":false,"given":"Herbert Magalh\u00e3es","family":"de Sousa","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Org\u00e2nica e Inorg\u00e2nica, Universidade Federal do Cear\u00e1, Fortaleza-CE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison Batista","family":"da Silva","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Org\u00e2nica e Inorg\u00e2nica, Universidade Federal do Cear\u00e1, Fortaleza-CE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Kueirislene Am\u00e2ncio","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Programa de Gradua\u00e7\u00e3o em Ci\u00eancias Naturais, Universidade Estadual de Cear\u00e1, Fortaleza-CE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Wlisses","family":"da Silva","sequence":"additional","affiliation":[{"name":"Programa de Gradua\u00e7\u00e3o em Biotecnologia, Universidade Estadual de Cear\u00e1, Fortaleza-CE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane Eire Silva Alencar","family":"de Menezes","sequence":"additional","affiliation":[{"name":"Programa de Gradua\u00e7\u00e3o em Ci\u00eancias Naturais, Universidade Estadual de Cear\u00e1, Fortaleza-CE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel Silva","family":"Marinho","sequence":"additional","affiliation":[{"name":"Programa de Gradua\u00e7\u00e3o em Ci\u00eancias Naturais, Universidade Estadual de Cear\u00e1, Fortaleza-CE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rcia Machado","family":"Marinho","sequence":"additional","affiliation":[{"name":"Curso de Qu\u00edmica, Universidade Estadual do Vale do Acara\u00fa, Sobral-CE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helcio Silva","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Curso de Qu\u00edmica, Universidade Estadual do Vale do Acara\u00fa, Sobral-CE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1617-7586","authenticated-orcid":false,"given":"Otilia Deusd\u00eania Loiola","family":"Pessoa","sequence":"additional","affiliation":[{"name":"Departamento 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