{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T11:04:02Z","timestamp":1773140642090,"version":"3.50.1"},"reference-count":27,"publisher":"Georg Thieme Verlag KG","issue":"08","funder":[{"DOI":"10.13039\/501100001700","name":"the Japanese Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"crossref","award":["16K09820"],"award-info":[{"award-number":["16K09820"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009619","name":"the Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["16ek0109043h0003"],"award-info":[{"award-number":["16ek0109043h0003"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Japanese Ministry of Health, Labor, and Welfare","award":["21FC1008"],"award-info":[{"award-number":["21FC1008"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:p>Autoimmune factor XIII (FXIII) deficiency (AiF13D) is an acquired life-threatening bleeding disorder due to anti-FXIII autoantibodies (autoAbs). We previously established an immunochromatographic test (ICT) for detection of anti-FXIII-A subunit (FXIII-A) autoAbs. Conversely, the detection of anti-FXIII-B subunit (FXIII-B) autoAbs is currently performed in a limited number of medical facilities through time-consuming and expensive laboratory tests, such as dot-blotting analysis and enzyme-linked immunosorbent assay (ELISA). Accordingly, in this study, we generated eight rat monoclonal antibodies (mAbs) against human FXIII-B using the rat lymph node method. By employing an ELISA, two mAbs, 2G12B10 and 8H12B9, were selected considering the distance between the recognition regions of each mAb (the 6th and 9th\u201310th Sushi domain, respectively) and the strength of their reactivity. Using this mAb combination, we prototyped an ICT to detect anti-FXIII-B autoAbs and distinguish between AiF13D and \u201cnonimmune\u201d acquired FXIII deficiency (acF13D), and tested it with 22 healthy controls, 23 acF13D patients, 15 AiF13D patients without anti-FXIII-B autoAbs, and 8 AiF13D patients with anti-FXIII-B autoAbs. Receiver operating characteristic curve analyses of ICTs for anti-FXIII-B autoAbs were performed and revealed a precision similar to dot-blot analysis. Human anti-FXIII-A mAbs were also generated from a single patient with AiF13D using a new cDNA cloning method, and their binding properties were characterized. Consequently, anti-FXIII-A immunoglobulin G preparations were established as potentially permanent positive controls of ICT for anti-FXIII-A antibodies. Combining the previously developed ICT for anti-FXIII-A autoAbs and the novel ICT for anti-FXIII-B autoAbs may reduce false negatives and lead to appropriate diagnosis and treatment.<\/jats:p>","DOI":"10.1055\/a-2061-3182","type":"journal-article","created":{"date-parts":[[2023,3,23]],"date-time":"2023-03-23T23:42:35Z","timestamp":1679614955000},"page":"793-803","source":"Crossref","is-referenced-by-count":5,"title":["Novel Immunochromatographic Test for Anti-factor XIII B Subunit Autoantibodies to Diagnose Autoimmune Acquired Factor XIII Deficiency"],"prefix":"10.1055","volume":"123","author":[{"given":"Tsukasa","family":"Osaki","sequence":"additional","affiliation":[{"name":"Department of Molecular Patho-Biochemistry and Patho-Biology, Yamagata University School of Medicine, Yamagata, Japan"},{"name":"The Japanese Collaborative Research Group (JCRG) on Autoimmune Acquired Coagulation Factor Deficiencies supported by the Japanese Ministry of Health, Labor and Welfare (MHLW), Yamagata, Japan"},{"name":"Department of Public Health and Hygiene, Yamagata University School of Medicine, Yamagata, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chikako","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"Department of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, Yonezawa, Japan"},{"name":"Department of Chemistry and Bioengineering, Graduate School of Engineering, Osaka Metropolitan University, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuo","family":"Magari","sequence":"additional","affiliation":[{"name":"Q-may Laboratory Corporation, Oita, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masayoshi","family":"Souri","sequence":"additional","affiliation":[{"name":"Department of Molecular Patho-Biochemistry and Patho-Biology, Yamagata University School of Medicine, Yamagata, Japan"},{"name":"The Japanese Collaborative Research Group (JCRG) on Autoimmune Acquired Coagulation Factor Deficiencies supported by the Japanese Ministry of Health, Labor and Welfare (MHLW), Yamagata, Japan"},{"name":"Department of Public Health and Hygiene, Yamagata University School of Medicine, Yamagata, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3807-5628","authenticated-orcid":false,"given":"Akitada","family":"Ichinose","sequence":"additional","affiliation":[{"name":"Department of Molecular Patho-Biochemistry and Patho-Biology, Yamagata University School of Medicine, Yamagata, Japan"},{"name":"The Japanese Collaborative Research Group (JCRG) on Autoimmune Acquired Coagulation Factor Deficiencies supported by the Japanese Ministry of Health, Labor and Welfare (MHLW), Yamagata, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2023,4,28]]},"reference":[{"issue":"07","key":"ref1","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1111\/j.1538-7836.2005.01213.x","article-title":"Factor XIII and the clotting of fibrinogen: from basic research to medicine","volume":"3","author":"L Lorand","year":"2005","journal-title":"J Thromb Haemost"},{"issue":"03","key":"ref2","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1152\/physrev.00016.2010","article-title":"Factor XIII: a coagulation factor with multiple plasmatic and cellular functions","volume":"91","author":"L Muszbek","year":"2011","journal-title":"Physiol Rev"},{"issue":"04","key":"ref3","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1007\/s12185-012-1064-3","article-title":"Factor XIII is a key molecule at the intersection of coagulation and fibrinolysis as well as inflammation and infection control","volume":"95","author":"A Ichinose","year":"2012","journal-title":"Int J Hematol"},{"issue":"04","key":"ref4","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1055\/s-0036-1571341","article-title":"Factor XIII: structure and function","volume":"42","author":"V Schroeder","year":"2016","journal-title":"Semin Thromb Hemost"},{"issue":"06","key":"ref5","doi-asserted-by":"crossref","first-page":"3055","DOI":"10.3390\/ijms22063055","article-title":"Factor XIII-A: an indispensable \u201cfactor\u201d in haemostasis and wound healing","volume":"22","author":"F SM Alshehri","year":"2021","journal-title":"Int J Mol Sci"},{"issue":"16","key":"ref6","doi-asserted-by":"crossref","first-page":"4633","DOI":"10.1021\/bi00364a027","article-title":"Amino acid sequence of the b subunit of human factor XIII, a protein composed of ten repetitive segments","volume":"25","author":"A 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