{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T04:26:17Z","timestamp":1781670377339,"version":"3.54.5"},"reference-count":26,"publisher":"Georg Thieme Verlag KG","issue":"06","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Klin Monbl Augenheilkd"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Zusammenfassung<\/jats:title><jats:p>\n          Hintergrund Der Keratokonus geht mit einer Ver\u00e4nderung der kornealen Biomechanik einher. Mittels Nanoindentation kann eine ortsaufgel\u00f6ste Charakterisierung biomechanischer\n          Eigenschaften an humanem Hornhautgewebe erfolgen. Ziel der Studie ist die Erfassung der biomechanischen Eigenschaften von Hornh\u00e4uten mit Keratokonus im Vergleich zu einer gesunden\n          Kontrollgruppe mit Spendergewebe, das nicht zur Transplantation geeignet ist.<\/jats:p><jats:p>\n          Methoden 17 Gewebeexplantate von Patienten mit Keratokonus und 10 gesunde, nicht f\u00fcr Transplantationszwecke geeignete Hornh\u00e4ute wurden in die Studie eingeschlossen. Alle Proben\n          wurden f\u00fcr mindestens 24\u2009h in Kulturmedium mit 15% Dextran \u00e4quilibriert. Anschlie\u00dfend erfolgte die Nanoindentation bis in eine Tiefe von 25\u2009\u00b5m bei einer Kraftzunahme von 300\u2009\u00b5N\/min.<\/jats:p><jats:p>\n          Ergebnisse Insgesamt wurden 2328 ortsaufgel\u00f6ste einzelne Nanoindentationsmessungen durchgef\u00fchrt. In der Keratokonusgruppe (1802 Indentationen) lag das mittlere Elastizit\u00e4tsmodul bei\n          23,2\u2009kPa (\u00b1\u200915,0\u2009kPa). In der Kontrollgruppe (526 Indentationen) lag das mittlere Elastizit\u00e4tsmodul bei 48,7\u2009kPa (\u00b1\u200920,5\u2009kPa). Im Wilcoxon-Test zeigte sich der Unterschied als statistisch\n          signifikant.<\/jats:p><jats:p>\n          Schlussfolgerung Mittels Nanoindentation konnte ex vivo bei Hornh\u00e4uten mit Keratokonus im Vergleich zu Hornh\u00e4uten ohne Keratokonus ein signifikant geringeres und damit weicheres\n          Elastizit\u00e4tsmodul gefunden werden. Diese Ergebnisse sind eine Basis f\u00fcr die Initiierung weiterer Studien, um ein besseres Verst\u00e4ndnis \u00fcber die pathologisch ver\u00e4nderte korneale Biomechanik\n          bei Keratokonus zu entwickeln.<\/jats:p>","DOI":"10.1055\/a-2062-3633","type":"journal-article","created":{"date-parts":[[2023,5,5]],"date-time":"2023-05-05T23:09:40Z","timestamp":1683328180000},"page":"774-778","source":"Crossref","is-referenced-by-count":6,"title":["Keratokonus: Biomechanik ex vivo"],"prefix":"10.1055","volume":"240","author":[{"given":"Robert","family":"Lohm\u00fcller","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Augenheilkunde, Albert-Ludwigs-Universit\u00e4t Freiburg, Medizinische Fakult\u00e4t, Deutschland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"B\u00f6hringer","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Augenheilkunde, Albert-Ludwigs-Universit\u00e4t Freiburg, Medizinische Fakult\u00e4t, Deutschland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip Christian","family":"Maier","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Augenheilkunde, Albert-Ludwigs-Universit\u00e4t Freiburg, Medizinische Fakult\u00e4t, Deutschland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea Karin","family":"Ross","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Augenheilkunde, Albert-Ludwigs-Universit\u00e4t Freiburg, Medizinische Fakult\u00e4t, Deutschland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0355-7766","authenticated-orcid":false,"given":"G\u00fcnther","family":"Schlunck","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Augenheilkunde, Albert-Ludwigs-Universit\u00e4t Freiburg, Medizinische Fakult\u00e4t, Deutschland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Reinhard","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Augenheilkunde, Albert-Ludwigs-Universit\u00e4t Freiburg, Medizinische Fakult\u00e4t, Deutschland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5324-7002","authenticated-orcid":false,"given":"Stefan J.","family":"Lang","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Augenheilkunde, Albert-Ludwigs-Universit\u00e4t Freiburg, Medizinische Fakult\u00e4t, Deutschland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2023,5,5]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1109","DOI":"10.1097\/ICO.0b013e31817f815a","article-title":"Keratoconus clinical findings according to different age and gender groups","volume":"27","author":"A Ertan","year":"2008","journal-title":"Cornea"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"1592","DOI":"10.1097\/ICO.0000000000002420","article-title":"Keratoconus in Children: A Literature Review","volume":"39","author":"L Buzzonetti","year":"2020","journal-title":"Cornea"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"1582","DOI":"10.1016\/j.jcrs.2017.08.024","article-title":"Vision-related quality of life and dependency in French keratoconus patients: Impact study","volume":"43","author":"V Saunier","year":"2017","journal-title":"J Cataract Refract Surg"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1111\/cxo.12300","article-title":"Contact lens management of keratoconus","volume":"98","author":"L E Downie","year":"2015","journal-title":"Clin Exp Optom"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1055\/s-0042-103494","article-title":"[Crosslinking in Keratoconus]","volume":"233","author":"S J Lang","year":"2016","journal-title":"Klin Monbl Augenheilkd"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"e112696","DOI":"10.1371\/journal.pone.0112696","article-title":"Analysis of the changes in keratoplasty indications and preferred techniques","volume":"9","author":"S J Lang","year":"2014","journal-title":"PLoS One"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1006\/exer.1997.0410","article-title":"Induction of cross-links in corneal tissue","volume":"66","author":"E Spoerl","year":"1998","journal-title":"Exp Eye Res"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1097\/01.ico.0000105827.85025.7f","article-title":"Collagen fiber diameter in the rabbit cornea after collagen crosslinking by riboflavin\/UVA","volume":"23","author":"G Wollensak","year":"2004","journal-title":"Cornea"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"620","DOI":"10.1016\/s0002-9394(02)02220-1","article-title":"Riboflavin\/ultraviolet-a-induced collagen crosslinking for the treatment of keratoconus","volume":"135","author":"G Wollensak","year":"2003","journal-title":"Am J Ophthalmol"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"812","DOI":"10.1016\/j.ophtha.2013.10.028","article-title":"A randomized, controlled trial of corneal collagen cross-linking in progressive keratoconus: three-year results","volume":"121","author":"C Wittig-Silva","year":"2014","journal-title":"Ophthalmology"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1186\/s12886-015-0070-7","article-title":"Prospective, randomized, double-blind trial to investigate the efficacy and safety of corneal cross-linking to halt the progression of keratoconus","volume":"15","author":"S J Lang","year":"2015","journal-title":"BMC Ophthalmol"},{"key":"ref12","doi-asserted-by":"publisher","first-page":"827","DOI":"10.1007\/s10792-015-0054-x","article-title":"Collagen cross-linking in keratoconus in Asian eyes: visual, refractive and confocal microscopy outcomes in a prospective randomized controlled trial","volume":"35","author":"N Sharma","year":"2015","journal-title":"Int Ophthalmol"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"1259","DOI":"10.1016\/j.ophtha.2017.03.052","article-title":"United States Multicenter Clinical Trial of Corneal Collagen Crosslinking for Keratoconus Treatment","volume":"124","author":"P S Hersh","year":"2017","journal-title":"Ophthalmology"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1097\/j.jcrs.0000000000000740","article-title":"Correlation of the Corvis Biomechanical Factor with tomographic parameters in keratoconus","volume":"48","author":"E Flockerzi","year":"2022","journal-title":"J Cataract Refract Surg"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1016\/s0014-4835(80)80027-3","article-title":"Biomechanical properties of keratoconus and normal corneas","volume":"31","author":"T T Andreassen","year":"1980","journal-title":"Exp Eye Res"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1167\/tvst.11.6.4","article-title":"Quantitative Assessment of Biomechanical Properties of the Human Keratoconus Cornea Using Acoustic Radiation Force Optical Coherence Elastography","volume":"11","author":"Y Zhao","year":"2022","journal-title":"Transl Vis Sci Technol"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"7467","DOI":"10.1038\/s41598-019-43811-5","article-title":"Spatially-resolved Brillouin spectroscopy reveals biomechanical abnormalities in mild to advanced keratoconus in vivo","volume":"9","author":"P Shao","year":"2019","journal-title":"Sci Rep"},{"key":"ref18","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1016\/j.ajo.2019.02.010","article-title":"Brillouin Spectroscopy of Normal and Keratoconus Corneas","volume":"202","author":"T G Seiler","year":"2019","journal-title":"Am J Ophthalmol"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1016\/j.actbio.2016.12.007","article-title":"A simple basis for determination of the modulus and hydraulic conductivity of human ocular surface using nano-indentation","volume":"50","author":"M V Swain","year":"2017","journal-title":"Acta Biomater"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"1413","DOI":"10.1002\/jbm.a.36337","article-title":"Instrumented indentation for determination of mechanical properties of human cornea after ultraviolet-A crosslinking","volume":"106","author":"J Nohava","year":"2018","journal-title":"J Biomed Mater Res A"},{"key":"ref21","doi-asserted-by":"publisher","first-page":"3282","DOI":"10.1167\/iovs.15-18248","article-title":"Measurement of an Elasticity Map in the Human Cornea","volume":"57","author":"E R Mikula","year":"2016","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref22","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.exer.2018.05.019","article-title":"Axial mechanical and structural characterization of keratoconus corneas","volume":"175","author":"E Mikula","year":"2018","journal-title":"Exp Eye Res"},{"key":"ref23","doi-asserted-by":"publisher","first-page":"810","DOI":"10.1007\/s00347-013-2818-x","article-title":"[New aspects on biomechanics of the cornea in keratoconus]","volume":"110","author":"Z Gatzioufas","year":"2013","journal-title":"Ophthalmologe"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"4324","DOI":"10.1167\/iovs.09-4798","article-title":"Age-related differences in the elasticity of the human cornea","volume":"52","author":"N E Knox Cartwright","year":"2011","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref25","doi-asserted-by":"publisher","first-page":"967","DOI":"10.1007\/s10792-019-01273-8","article-title":"Corneal biomechanics in different age groups","volume":"40","author":"A AK El Massry","year":"2020","journal-title":"Int Ophthalmol"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"108839","DOI":"10.1016\/j.exer.2021.108839","article-title":"Corneal biomechanical properties following corneal cross-linking: Does age have an effect?","volume":"214","author":"B Alenezi","year":"2022","journal-title":"Exp Eye Res"}],"container-title":["Klinische Monatsbl\u00e4tter f\u00fcr Augenheilkunde"],"original-title":[],"language":"de","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-2062-3633.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,23]],"date-time":"2023-06-23T09:10:13Z","timestamp":1687511413000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-2062-3633"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,5]]},"references-count":26,"journal-issue":{"issue":"06","published-online":{"date-parts":[[2023,6,22]]},"published-print":{"date-parts":[[2023,6]]}},"URL":"https:\/\/doi.org\/10.1055\/a-2062-3633","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["0023-2165","1439-3999"],"issn-type":[{"value":"0023-2165","type":"print"},{"value":"1439-3999","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,5]]}}}