{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T07:23:32Z","timestamp":1772609012050,"version":"3.50.1"},"reference-count":40,"publisher":"Georg Thieme Verlag KG","issue":"08","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Horm Metab Res"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Basement membranes (BMs) are widely distributed and highly specialized\n                    extracellular matrix (ECM). The goal of this study was to explore novel genes\n                    associated with nonalcoholic fatty liver disease (NAFLD) from the perspective of\n                    BMs. Sequencing results of 304 liver biopsy samples about NAFLD were\n                    systematically obtained from the Gene Expression Omnibus (GEO) database.\n                    Biological changes during NAFLD progression and hub BM-associated genes were\n                    investigated by differential gene analysis and weighted gene co-expression\n                    network analysis (WGCNA), respectively. The nonalcoholic steatohepatitis (NASH)\n                    subgroups were identified based on hub BM-associated genes expression, as well\n                    as the differences in Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling\n                    pathways and immune microenvironment between different subgroups were compared.\n                    Extracellular matrix (ECM) seems to play an important role in the development of\n                    NAFLD. Three representative BM-associated genes (ADAMTS2, COL5A1, and LAMC3)\n                    were finally identified. Subgroup analysis results suggested that there were\n                    significant changes in KEGG signaling pathways related to metabolism,\n                    extracellular matrix, cell proliferation, differentiation, and death. There were\n                    also changes in macrophage polarization, neutrophils, and dendritic cells\n                    abundance, and so on. In conclusion, the present study identified novel\n                    potential BM-associated biomarkers and further explored the heterogeneity of\n                    NASH that might provide new insights into the diagnosis, assessment, management,\n                    and personalized treatment of NAFLD.<\/jats:p>","DOI":"10.1055\/a-2081-1098","type":"journal-article","created":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T23:03:02Z","timestamp":1685746982000},"page":"546-554","source":"Crossref","is-referenced-by-count":3,"title":["Identification of Basement Membrane Genes and Related Molecular\n                    Subtypes in Nonalcoholic Fatty Liver Disease"],"prefix":"10.1055","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6577-0565","authenticated-orcid":false,"given":"Zhaoxiang","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Endocrinology, Affiliated Hospital of Jiangsu University,\n                    Zhenjiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huijuan","family":"Qin","sequence":"first","affiliation":[{"name":"Department of Endocrinology, Affiliated Hospital of Jiangsu University,\n                    Zhenjiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qichao","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Jiangsu University Affiliated Wujin\n                    Hospital, Changzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jue","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Affiliated Hospital of Jiangsu University,\n                    Zhenjiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Affiliated Hospital of Jiangsu University,\n                    Zhenjiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shao","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Affiliated Kunshan Hospital of Jiangsu\n                    University, Kunshan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoyue","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Affiliated Hospital of Jiangsu University,\n                    Zhenjiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2023,6,2]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"2212","DOI":"10.1016\/S0140-6736(20)32511-3","article-title":"Non-alcoholic fatty liver disease","volume":"397","author":"E E Powell","year":"2021","journal-title":"Lancet"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1001\/jama.2020.2298","article-title":"Nonalcoholic 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Dis"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1002\/hep.1840040612","article-title":"Basement membrane production by hepatocytes in chronic liver disease","volume":"4","author":"F B Bianchi","year":"1984","journal-title":"Hepatology"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"D991","DOI":"10.1093\/nar\/gks1193","article-title":"NCBI GEO: archive for functional genomics data sets--update","volume":"41","author":"T Barrett","year":"2013","journal-title":"Nucleic Acids Res"},{"key":"ref12","first-page":"2","article-title":"ComBat-seq: batch effect adjustment for RNA-seq count data","author":"Y Zhang","year":"2020","journal-title":"NAR Genom Bioinform"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"2044","DOI":"10.1093\/bib\/bby067","article-title":"Interpretation of differential gene expression results of RNA-seq data: review\n                    and integration","volume":"20","author":"A McDermaid","year":"2019","journal-title":"Brief Bioinform"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1186\/1471-2105-9-559","article-title":"WGCNA: an R package for weighted correlation network analysis","volume":"9","author":"P Langfelder","year":"2008","journal-title":"BMC Bioinformatics"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1089\/omi.2011.0118","article-title":"clusterProfiler: an R package for comparing biological themes among gene\n                    clusters","volume":"16","author":"G Yu","year":"2012","journal-title":"OMICS"},{"key":"ref16","first-page":"12","article-title":"Logistic LASSO regression for dietary Intakes and breast cancer","author":"A J McEligot","year":"2020","journal-title":"Nutrients"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"15545","DOI":"10.1073\/pnas.0506580102","article-title":"Gene set enrichment analysis: a knowledge-based approach for interpreting\n                    genome-wide expression profiles","volume":"102","author":"A Subramanian","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1016\/j.eururo.2018.08.038","article-title":"Reporting and interpreting decision curve analysis: a guide for\n                    investigators","volume":"74","author":"B Van Calster","year":"2018","journal-title":"Eur Urol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1572","DOI":"10.1093\/bioinformatics\/btq170","article-title":"ConsensusClusterPlus: a class discovery tool with confidence assessments and\n                    item tracking","volume":"26","author":"M D Wilkerson","year":"2010","journal-title":"Bioinformatics"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1471-2105-14-7","article-title":"GSVA: gene set variation analysis for microarray and RNA-seq data","volume":"14","author":"S H\u00e4nzelmann","year":"2013","journal-title":"BMC Bioinformatics"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1038\/nmeth.3337","article-title":"Robust enumeration of cell subsets from tissue expression profiles","volume":"12","author":"A M Newman","year":"2015","journal-title":"Nat Methods"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"967016","DOI":"10.3389\/fendo.2022.967016","article-title":"Comprehensive analysis of endoplasmic reticulum-related and secretome gene\n                    expression profiles in the progression of non-alcoholic fatty liver disease","volume":"13","author":"R Gao","year":"2022","journal-title":"Front Endocrinol (Lausanne)"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1379","DOI":"10.1007\/s40618-022-01773-y","article-title":"Potential biomarkers in the fibrosis progression of nonalcoholic steatohepatitis\n                    (NASH)","volume":"45","author":"Z Wang","year":"2022","journal-title":"J 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Hepatol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1038\/nrm3904","article-title":"Remodelling the extracellular matrix in development and disease","volume":"15","author":"C Bonnans","year":"2014","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.matbio.2015.04.001","article-title":"The procollagen N-proteinases ADAMTS2, 3 and 14 in pathophysiology","volume":"44-46","author":"M Bekhouche","year":"2015","journal-title":"Matrix Biol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.jhep.2008.03.020","article-title":"Hepatic expression of A disintegrin and metalloproteinase (ADAM) and ADAMs with\n                    thrombospondin motives (ADAM-TS) enzymes in patients with chronic liver\n                    diseases","volume":"49","author":"L Schwettmann","year":"2008","journal-title":"J 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Gu","year":"2021","journal-title":"Front Oncol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"8101","DOI":"10.1002\/jcb.28089","article-title":"A novel messenger RNA and long noncoding RNA signature associated with the\n                    progression of nonmuscle invasive bladder cancer","volume":"120","author":"Y H He","year":"2019","journal-title":"J Cell Biochem"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1038\/s41591-018-0104-9","article-title":"Mechanisms of NAFLD development and therapeutic strategies","volume":"24","author":"S L Friedman","year":"2018","journal-title":"Nat Med"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1784","DOI":"10.1053\/j.gastro.2015.08.011","article-title":"Heritability of hepatic fibrosis and steatosis based on a prospective twin\n                    study","volume":"149","author":"R Loomba","year":"2015","journal-title":"Gastroenterology"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"2697","DOI":"10.1172\/JCI93465","article-title":"Nonalcoholic fatty liver disease with cirrhosis increases familial risk for\n                    advanced fibrosis","volume":"127","author":"C Caussy","year":"2017","journal-title":"J Clin Invest"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1038\/s41575-020-0304-x","article-title":"Single-cell technologies in hepatology: new insights into liver biology and\n                    disease pathogenesis","volume":"17","author":"P Ramachandran","year":"2020","journal-title":"Nat Rev Gastroenterol Hepatol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1016\/j.jhep.2022.06.012","article-title":"Immune mechanisms linking metabolic injury to inflammation and fibrosis in fatty\n                    liver disease - novel insights into cellular communication circuits","volume":"77","author":"M Peiseler","year":"2022","journal-title":"J Hepatol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1038\/s41588-021-00894-z","article-title":"Single-nucleus chromatin accessibility and transcriptomic characterization of\n                    Alzheimer\u2019s disease","volume":"53","author":"S Morabito","year":"2021","journal-title":"Nat Genet"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.1016\/j.cell.2019.07.021","article-title":"LECT2, a ligand for tie1, plays a crucial role in liver Ffibrogenesis","author":"M Xu","year":"2019","journal-title":"Cell"}],"container-title":["Hormone and Metabolic Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-2081-1098.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T23:03:02Z","timestamp":1690844582000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-2081-1098"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,2]]},"references-count":40,"journal-issue":{"issue":"08","published-online":{"date-parts":[[2023,7,31]]},"published-print":{"date-parts":[[2023,8]]}},"URL":"https:\/\/doi.org\/10.1055\/a-2081-1098","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["0018-5043","1439-4286"],"issn-type":[{"value":"0018-5043","type":"print"},{"value":"1439-4286","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,2]]}}}