{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,20]],"date-time":"2025-07-20T04:24:53Z","timestamp":1752985493248,"version":"3.37.3"},"reference-count":46,"publisher":"Georg Thieme Verlag KG","issue":"01","funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"crossref","award":["848290"],"award-info":[{"award-number":["848290"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The development of virus-free, oral vaccines against poliovirus capable of inducing mucosal protective immunity is needed to safely combat this pathogen. In the present study, a carrot cell\n          line expressing the poliovirus VP2 antigen was established at the level of callus and cell suspensions, exploring the effects of culture media (MS and B5), supplementation with urea,\n          phytoregulators (2,4-D\u200a:\u200aKIN), and light conditions (continuous light, photoperiod, and total darkness). The best callus growth was obtained on B5 medium supplemented with 2\u2009mg\/L of\n          2,4-D\u2009+\u20092\u2009mg\/L kinetin and 0.0136\u2009g\/L of urea and in continuous light conditions. Suspension cultures of the SMC-1 line in 250\u2009mL Erlenmeyer flasks had a maximum growth of 16.07\u2009\u00b1\u20090.03\u2009g\/L\n          DW on day 12 with a growth rate of \u00b5=0.3\/d and a doubling time of 2.3 days. In a 2\u2009L airlift bioreactor, the biomass yield achieved was 25.6\u2009\u00b1\u20090.05\u2009g\/L DW at day 10 with a growth rate of \u00b5=\n          0.58\/d and doubling time of 1.38\u2009d. Cell growth was 1.5 times higher in bioreactors than in shake flasks, highlighting that both systems resulted in the accumulation of VP2 throughout the\n          time in culture. The maximum VP2 yield in flasks was 387.8\u2009\u00b5g\/g DW at day 21, while in the reactor it was 550.2\u2009\u00b5g\/g DW at day 18. In conclusion, bioreactor-based production of the VP2\n          protein by the SMC-1 suspension cell line offers a higher productivity when compared to flask cultures, offering a key perspective to produce low-cost vaccines against poliomyelitis.<\/jats:p>","DOI":"10.1055\/a-2181-2886","type":"journal-article","created":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T22:49:37Z","timestamp":1697669377000},"page":"63-72","source":"Crossref","is-referenced-by-count":2,"title":["Establishment of the Daucus carota SMC-1 Cell Suspension Line for Poliovirus Vaccine Development"],"prefix":"10.1055","volume":"90","author":[{"given":"M\u00f3nica","family":"Morales-Aguilar","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda, Universidad Aut\u00f3noma del Estado de Morelos, Cuernavaca, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omayra Citlalli","family":"Bola\u00f1os-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Laboratorio de Biofarmac\u00e9uticos Recombinantes, Universidad Aut\u00f3noma de San Luis Potos\u00ed, SLP, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea Romero","family":"Maldonado","sequence":"additional","affiliation":[{"name":"Laboratorio de Biofarmac\u00e9uticos Recombinantes, Universidad Aut\u00f3noma de San Luis Potos\u00ed, SLP, Mexico"},{"name":"Secci\u00f3n de Biotecnolog\u00eda, Centro de Investigaci\u00f3n en Ciencias de la Salud y Biomedicina (CICSaB), Universidad Aut\u00f3noma de San Luis Potos\u00ed, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dania O.","family":"Govea-Alonso","sequence":"additional","affiliation":[{"name":"Laboratorio de Biofarmac\u00e9uticos Recombinantes, Universidad Aut\u00f3noma de San Luis Potos\u00ed, SLP, Mexico"},{"name":"Secci\u00f3n de Biotecnolog\u00eda, Centro de Investigaci\u00f3n en Ciencias de la Salud y Biomedicina (CICSaB), Universidad Aut\u00f3noma de San Luis Potos\u00ed, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Carre\u00f1o-Campos","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda, Universidad Aut\u00f3noma del Estado de Morelos, Cuernavaca, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Luisa","family":"Villarreal","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda, Universidad Aut\u00f3noma del Estado de Morelos, Cuernavaca, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Rosales-Mendoza","sequence":"additional","affiliation":[{"name":"Laboratorio de Biofarmac\u00e9uticos Recombinantes, Universidad Aut\u00f3noma de San Luis Potos\u00ed, SLP, Mexico"},{"name":"Secci\u00f3n de Biotecnolog\u00eda, Centro de Investigaci\u00f3n en Ciencias de la Salud y Biomedicina (CICSaB), Universidad Aut\u00f3noma de San Luis Potos\u00ed, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anabel","family":"Ortiz-Caltempa","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda, Universidad Aut\u00f3noma del Estado de Morelos, Cuernavaca, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2023,10,18]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1128\/MMBR.00012-14","article-title":"Picornavirus morphogenesis","volume":"78","author":"P Jiang","year":"2014","journal-title":"Microbiol Mol Biol Rev"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/0042-6822(83)90297-0","article-title":"Identification of a poliovirus neutralization epitope through use of neutralizing antiserum raised against a purified 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