{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T06:03:27Z","timestamp":1785737007853,"version":"3.56.0"},"reference-count":38,"publisher":"Georg Thieme Verlag KG","issue":"02","funder":[{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research, India","doi-asserted-by":"crossref","award":["CII7041"],"award-info":[{"award-number":["CII7041"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research, India","doi-asserted-by":"crossref","award":["MLP2028"],"award-info":[{"award-number":["MLP2028"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research, India","doi-asserted-by":"crossref","award":["MLP2035"],"award-info":[{"award-number":["MLP2035"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Oxystelma esculentum has been used as a folk medicine to treat jaundice, throat infections, and skin problems. In the current study, the bone fracture-healing properties of a\n          flavonoid-enriched fraction (Oxy50-60F) of O. esculentum were investigated in Swiss mice using a drill-hole injury model. Oxy50-60F (1\u2009mg\/kg\/day, 5\u2009mg\/kg\/day, and 10\u2009mg\/kg\/day) was\n          administered orally (from the next day) after a 0.6\u2009mm drill-hole injury in mice femur mid-diaphysis for 7 days and 14 days. Parathyroid hormone (40\u2009\u00b5g\/kg; 5 times\/week) was given\n          subcutaneously as the positive control. Confocal imaging for bone regeneration, micro-architecture of femur bones, ex vivo mineralization, hematoxyline and eosin staining, measurement\n          of reactive oxygen species, and gene expression of osteogenic and anti-inflammatory genes were studied. Quercetin, kaempferol, and isorhamnetin glycosides were identified in the active\n          fraction using mass spectrometry techniques. Our results confirm that Oxy50-60F treatment promotes fracture healing and callus formation at drill-hole sites and stimulates osteogenic and\n          anti-inflammatory genes. Oxy50-60F administration to fractured mice exhibited significantly better micro-CT parameters in a dose-dependent manner and promoted nodule mineralization at days 7\n          and 14 post-injury. Oxy50-60F also prevents ROS generation by increasing expression of the SOD2 enzyme. Overall, this study reveals that Oxy50-60F has bone regeneration potential in a\n          cortical bone defect model, which supports its use in delayed-union and non-union fracture cases.<\/jats:p>","DOI":"10.1055\/a-2192-2138","type":"journal-article","created":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T07:54:51Z","timestamp":1697529291000},"page":"96-110","source":"Crossref","is-referenced-by-count":4,"title":["Bone Fracture-healing Properties and UPLC-MS Analysis of an Enriched Flavonoid Fraction from Oxystelma esculentum"],"prefix":"10.1055","volume":"90","author":[{"given":"Priyanka","family":"Rawat","sequence":"first","affiliation":[{"name":"Sophisticated Analytical Instrument Facility & Research, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India"},{"name":"Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kriti","family":"Sharma","sequence":"first","affiliation":[{"name":"Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India"},{"name":"Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonu","family":"Khanka","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India"},{"name":"Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yatendra","family":"Singh","sequence":"additional","affiliation":[{"name":"Sophisticated Analytical Instrument Facility & Research, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravi","family":"Prakash","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nisha","family":"Rais","sequence":"additional","affiliation":[{"name":"Sophisticated Analytical Instrument Facility & Research, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arvind Kumar","family":"Maurya","sequence":"additional","affiliation":[{"name":"Sophisticated Analytical Instrument Facility & Research, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India"},{"name":"Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ritika","family":"Gupta","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dipak K.","family":"Mishra","sequence":"additional","affiliation":[{"name":"Sophisticated Analytical Instrument Facility & Research, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India"},{"name":"Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Divya","family":"Singh","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India"},{"name":"Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2831-2797","authenticated-orcid":false,"given":"Sanjeev","family":"Kanojiya","sequence":"additional","affiliation":[{"name":"Sophisticated Analytical Instrument Facility & Research, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India"},{"name":"Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2023,11,10]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"87","DOI":"10.5530\/pj.2011.19.16","article-title":"A complete review on Oxystelma esculentum R. 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A preliminary report","volume":"366","author":"G Holzer","year":"1999","journal-title":"Clin Orthop Relat Res"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1172\/JCI11089","article-title":"Interactions between immune and bone cells: new insights with many remaining questions","volume":"106","author":"J Lorenzo","year":"2000","journal-title":"J Clin Invest"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1111\/jphp.12764","article-title":"Heartwood extract from Dalbergia sissoo promotes fracture healing and its application in ovariectomy-induced osteoporotic rats","volume":"69","author":"A Karvande","year":"2017","journal-title":"J Pharm Pharmacol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1007\/s00223-021-00818-3","article-title":"Phenanthrenoid coelogin isolated from coelogyne cristata exerts osteoprotective effect through MAPK-mitogen-activated protein kinase signaling pathway","volume":"109","author":"R Prakash","year":"2021","journal-title":"Calcif Tissue Int"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.jep.2019.01.029","article-title":"Extract and fraction of Cassia occidentalis L. 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