{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T07:26:21Z","timestamp":1787729181744,"version":"build-2784847793"},"reference-count":32,"publisher":"Georg Thieme Verlag KG","issue":"05","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Horm Metab Res"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Anaplastic thyroid carcinoma (ATC) is an aggressive malignancy threatening\n                    patients\u2019 life quality. Our previous study has demonstrated that\n                    inhibition of long non-coding RNA H19 (lncRNA h19; H19) blocks ATC growth and\n                    metastasis. The current study aimed to further explore the potential mechanism\n                    of H19 in ATC. Expression of H19, miR-454-3p, and BHLHE40 mRNA was measured\n                    using RT-qPCR in tissue samples and cell lines. The dual-luciferase reporter\n                    assay and Pearson correlation analysis were used to explore the interaction\n                    among H19, miR-454-3p, and BHLHE40. The biological process of proliferation,\n                    migration, and invasion was determined using loss- or gain-function CCK-8 and\n                    Transwell assays. Western blot assay was used to evaluate the changes in protein\n                    levels. H19 was elevated in ATC tissues and cell lines. Based on online\n                    prediction database results, miR-454-3p might be a target of H19, and BHLHE40\n                    might be a direct target of miR-454-3p. miR-454-3p expression was decreased in\n                    ATC and had a negative interaction with H19. BHLHE40 mRNA expression was\n                    increased and has a negative correlation with miR-454-3p and a positive\n                    correlation with H19. Downregulation of miR-454-3p and upregulation of BHLHE40\n                    could reverse the decreased cellular activities caused by si-H19. Moreover, the\n                    silence of H19 modulates BHLHE40 to affect the PI3K\/AKT protein levels\n                    and apoptotic-related protein levels. The current study provided a potential\n                    detailed mechanism of H19 in ATC, and lncRNA H19-miR-454-3p-BHLHE40 interaction\n                    may be a new experimental basis for prognosis and targeted therapy for ATC\n                    patients.<\/jats:p>","DOI":"10.1055\/a-2196-3511","type":"journal-article","created":{"date-parts":[[2023,12,6]],"date-time":"2023-12-06T00:01:50Z","timestamp":1701820910000},"page":"392-399","source":"Crossref","is-referenced-by-count":6,"title":["LncRNA H19 Influences Cellular Activities via the\n                    miR-454-3p\/BHLHE40 Axis in Anaplastic Thyroid Carcinoma"],"prefix":"10.1055","volume":"56","author":[{"given":"Yang","family":"Wu","sequence":"first","affiliation":[{"name":"Department of General Surgery (Thyroid Gland\/Blood Vessel), The\n                    First People\u2019s Hospital of Neijiang, Neijiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jihua","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Ultrasound, Central Hospital Affiliated to Shandong First\n                    Medical University, Jinan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honglai","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, Medical College of Soochow University,\n                    Suzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Oncology Radiotherapy, The Affiliated Hospital of Qingdao\n                    University, Qingdao, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peijie","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Oncology Radiotherapy, The Affiliated Hospital of Qingdao\n                    University, Qingdao, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianyuan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of General Surgery (Thyroid Gland\/Blood Vessel), The\n                    First People\u2019s Hospital of Neijiang, Neijiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2023,12,5]]},"reference":[{"key":"ref1","first-page":"239","article-title":"Anaplastic thyroid carcinoma: updates on WHO classification, clinicopathological\n                    features and staging","volume":"36","author":"I Abe","year":"2021","journal-title":"Histol Histopathol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"769807","DOI":"10.3389\/fonc.2021.769807","article-title":"Bibliometric insights in advances of anaplastic thyroid cancer: research\n                    landscapes, turning points, and global trends","volume":"11","author":"H 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