{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T03:25:14Z","timestamp":1793157914043,"version":"4.5.0"},"reference-count":41,"publisher":"Georg Thieme Verlag KG","issue":"02","license":[{"start":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:00:00Z","timestamp":1698710400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"XPCC Science and Technology Plan of Science and Technology Innovation Talent Program","award":["2022CB002\u201309"],"award-info":[{"award-number":["2022CB002\u201309"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Neurol Surg A Cent Eur Neurosurg"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Background\u2003Pituitary adenoma (PA) is a common intracranial endocrine tumor, but no precise target has been found for effective prediction and treatment of PA.<\/jats:p><jats:p>\n          Methods\u2003Quantitative reverse transcription polymerase chain reaction (qRT\u2012PCR) analysis showed that circMFN2 could affect the expression of miR-146a-3p in PA samples. Moreover, we used Western blotting to evaluate the expression levels of TRAF6 and NF-\u03baB markers. The EdU assay, scratch wound healing assay, and Matrigel invasion assay were performed to assess the potential function of this pathway in PA cells. Based on the bioinformatic analysis including KEGG, gene ontology (GO) analysis, and microarray analysis, we evaluated the efficacy of circMFN2 as a potential biomarker for diagnosing PA, and we aimed to determine the mechanism of action in PA cells.<\/jats:p><jats:p>\n          Results\u2003Our findings indicate that there is a significant increase in the expression of circMFN2 in tissues, serum, and exosomes in the invasive group compared with the noninvasive and normal groups. Furthermore, this difference was statistically significant both preoperatively and postoperatively. To clarify its function, we downregulated this gene, and the experimental results suggested that the motility and proliferative capacity were reduced in vitro. In addition, rescue assays showed that miR-146a-3p could successfully reverse the inhibitory effect of circMFN2 knockdown on motility and proliferation in PA cells. Moreover, downregulation of circMFN2 and miR-146a-3p significantly changed the expression of TRAF6 and NF-\u03baB.<\/jats:p><jats:p>\n          Conclusion\u2003This study identified that circMFN2 regulates miR-146a-3p to promote adenoma development partially via the TRAF6\/NF-\u03baB pathway and may be a potential therapeutic target for PA.<\/jats:p>","DOI":"10.1055\/a-2201-8370","type":"journal-article","created":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T01:01:31Z","timestamp":1698800491000},"page":"135-147","source":"Crossref","is-referenced-by-count":6,"title":["Exosomal CircMFN2 Enhances the Progression of Pituitary Adenoma via the MiR-146a-3p\/TRAF6\/NF-\u03baB Pathway"],"prefix":"10.1055","volume":"86","author":[{"given":"Haitong","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, First Affiliated Hospital of Medical College, Shihezi University, Shihezi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, First Affiliated Hospital of Medical College, Shihezi University, Shihezi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zexu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, First Affiliated Hospital of Medical College, Shihezi University, Shihezi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leiguo","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, First Affiliated Hospital of Medical College, Shihezi University, Shihezi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufei","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, First Affiliated Hospital of Medical College, Shihezi University, Shihezi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5504-3687","authenticated-orcid":false,"given":"Qi","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, First Affiliated Hospital of Medical College, Shihezi University, Shihezi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2024,2,16]]},"reference":[{"issue":"10","key":"ref1","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1056\/NEJMra1810772","article-title":"Pituitary-tumor endocrinopathies","volume":"382","author":"S Melmed","year":"2020","journal-title":"N Engl J Med"},{"issue":"02","key":"ref2","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1007\/s11154-019-09534-8","article-title":"Aggressive pituitary tumors in the young and elderly","volume":"21","author":"C Tatsi","year":"2020","journal-title":"Rev Endocr Metab 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