{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T12:37:08Z","timestamp":1764333428864,"version":"3.37.3"},"reference-count":42,"publisher":"Georg Thieme Verlag KG","issue":"07\/08","license":[{"start":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T00:00:00Z","timestamp":1717632000000},"content-version":"vor","delay-in-days":5,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Benzylisoquinoline alkaloids are the major bioactive components in Chelidonium majus, a plant that has a long usage history for the treatment of gastrointestinal ailments in European and Asian phytomedicine. This study reports on the development and application of a supercritical fluid chromatography technique for the simultaneous qualitative and quantitative determination of seven benzylisoquinoline alkaloids in under six minutes using a Viridis BEH 2-EP column and a modifier comprising methanol with 30% acetonitrile and 20\u2009mM ammonium formate. The method was fully validated according to ICH guidelines showing, e.g., excellent linearity (\u2265\u20090.9997) and maximum deviations for intraday and inter-day precision of 2.99 and 2.76%, respectively. The new supercritical fluid chromatography assay was not only employed for the analysis of several C. majus samples but was also used for the subsequent development of a fast centrifugal partition chromatography technique,\n          whereby five benzylisoquinoline alkaloids could be isolated within approximately 2.5\u2009h, with only two of them, protopine and chelidonine, requiring an additional purification step. To achieve this, a solvent system composed of chloroform\/methanol\/0.3\u2009M hydrochloric acid was used in descending mode. By injecting 500\u2009mg of crude extract, stylopine (1.93\u2009mg), sanguinarine (0.57\u2009mg), chelidonine (1.29\u2009mg), protopine (1.95\u2009mg), and coptisine (7.13\u2009mg) could be obtained. The purity of compounds was confirmed by supercritical fluid chromatography and MS.<\/jats:p>","DOI":"10.1055\/a-2204-5686","type":"journal-article","created":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T22:34:57Z","timestamp":1717713297000},"page":"523-533","source":"Crossref","is-referenced-by-count":4,"title":["Novel Approaches for the Analysis and Isolation of Benzylisoquinoline Alkaloids in Chelidonium majus"],"prefix":"10.1055","volume":"90","author":[{"given":"Michael","family":"Zwerger","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Pharmacognosy, Center for Molecular Biosciences (CMBI), University of Innsbruck, Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynda","family":"Boeck","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Pharmacognosy, Center for Molecular Biosciences (CMBI), University of Innsbruck, Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Manzl","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Pharmacognosy, Center for Molecular Biosciences (CMBI), University of Innsbruck, Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3731-7349","authenticated-orcid":false,"given":"Stefan","family":"Schwaiger","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Pharmacognosy, Center for Molecular Biosciences (CMBI), University of Innsbruck, Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Ganzera","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Pharmacognosy, Center for Molecular Biosciences (CMBI), University of Innsbruck, Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2024,6,6]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"299","DOI":"10.3389\/fphar.2018.00299","article-title":"Greater celandine\u02bcs ups and downs\u201321 centuries of medicinal uses of Chelidonium majus from the viewpoint of today\u02bcs pharmacology","volume":"9","author":"S Zielinska","year":"2018","journal-title":"Front Pharmacol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0021-9673(95)00643-1","article-title":"Application of \u03b2-cyclodextrin for the analysis of the main alkaloids from Chelidonium majus by capillary electrophoresis","volume":"717","author":"H Stuppner","year":"1995","journal-title":"J Chromatogr A"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1159\/000485410","article-title":"STW 5 (Iberogast) therapy in gastrointestinal functional 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Wang by high speed counter-current chromatography","volume":"28","author":"S Tong","year":"2005","journal-title":"J Liq Chromatogr Relat Technol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"411","DOI":"10.4236\/ajac.2011.24050","article-title":"An economical method for preparative purification of five alkaloids from Coptis chinensis Franch by high-speed counter-current chromatography using singled prepared solvent system by GC","volume":"02","author":"L Yin","year":"2011","journal-title":"AJAC"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"842","DOI":"10.3390\/molecules25040842","article-title":"Modulatory effect of Chelidonium majus extract and its alkaloids on LPS-stimulated cytokine secretion in human neutrophils","volume":"25","author":"S Zielinska","year":"2020","journal-title":"Molecules"}],"container-title":["Planta Medica"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-2204-5686.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T13:42:18Z","timestamp":1730814138000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-2204-5686"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6]]},"references-count":42,"journal-issue":{"issue":"07\/08","published-online":{"date-parts":[[2024,6,6]]},"published-print":{"date-parts":[[2024,6]]}},"URL":"https:\/\/doi.org\/10.1055\/a-2204-5686","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["0032-0943","1439-0221"],"issn-type":[{"type":"print","value":"0032-0943"},{"type":"electronic","value":"1439-0221"}],"subject":[],"published":{"date-parts":[[2024,6]]}}}