{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T14:14:01Z","timestamp":1781014441685,"version":"3.54.1"},"reference-count":34,"publisher":"Georg Thieme Verlag KG","issue":"01","license":[{"start":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T00:00:00Z","timestamp":1700697600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["AJP Rep"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Objective\u2003The objective of this study was to determine the cytokine response in human pregnant preterm and term myometrial cells exposed to lipopolysaccharide (LPS) and cocultured with mesenchymal stem cells (MSCs).<\/jats:p><jats:p>\n          Study Design\u2003Myometrium was obtained at cesarean delivery in term and preterm patients. Human myometrial cells were exposed to 5\u2009\u03bcg\/mL LPS for 4\u2009hours followed by 1\u2009\u03bcg\/mL LPS for 24\u2009hours and were cocultured with MSCs for 24\u2009hours. Culture supernatants were collected at 24\u2009hours and expression of cytokines, including interleukin-1\u03b2 (IL-1\u03b2), IL-6, IL-8, tumor necrosis factor-\u03b1 (TNF-\u03b1), transforming growth factor-\u03b2 (TGF-\u03b2), and IL-10, was quantified by enzyme-linked immunosorbent assay.<\/jats:p><jats:p>\n          Results\u2003There was significantly increased expression of the proinflammatory cytokines IL-1\u03b2, IL-6, IL-8, and TNF-\u03b1 in preterm myometrial cells treated with LPS compared with untreated preterm myometrial cells. Coculture with MSCs significantly suppressed the proinflammatory cytokine levels in LPS-treated preterm versus treated term myometrial cells. Moreover, MSC cocultured preterm myometrial cells expressed increased levels of the anti-inflammatory cytokines TGF-\u03b2 and IL-10 compared with treated term myometrial cells.<\/jats:p><jats:p>\n          Conclusion\u2003MSCs ameliorate LPS-mediated inflammation in preterm human myometrial cells compared with term myometrial cells. Immunomodulatory effects of MSCs mediated through anti-inflammatory cytokine regulation suggest a potential cell-based therapy for preterm birth.<\/jats:p>","DOI":"10.1055\/a-2216-9194","type":"journal-article","created":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T23:57:41Z","timestamp":1700783861000},"page":"e69-e73","source":"Crossref","is-referenced-by-count":3,"title":["Mesenchymal Stem Cells Suppress Inflammatory Cytokines in Lipopolysaccharide Exposed Preterm and Term Human Pregnant Myometrial Cells"],"prefix":"10.1055","volume":"14","author":[{"given":"Arunmani","family":"Mani","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Gynecology and Reproductive Sciences, University of Texas Health Science Center at Houston, Houston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Hotra","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Gynecology and Reproductive Sciences, University of Texas Health Science Center at Houston, Houston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean C.","family":"Blackwell","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Texas Health Science Center at Houston, Houston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Goetzl","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Gynecology and Reproductive Sciences, University of Texas Health Science Center at Houston, Houston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerrie S.","family":"Refuerzo","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Gynecology and Reproductive Sciences, University of Texas Health Science Center at Houston, Houston, 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