{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T12:48:20Z","timestamp":1789130900619,"version":"build-2803163510"},"reference-count":23,"publisher":"Georg Thieme Verlag KG","issue":"07","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82261138554"],"award-info":[{"award-number":["82261138554"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82170130"],"award-info":[{"award-number":["82170130"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81970124"],"award-info":[{"award-number":["81970124"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81400082"],"award-info":[{"award-number":["81400082"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"crossref","award":["BK20140219"],"award-info":[{"award-number":["BK20140219"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Distinguished Professorship Program of Jiangsu Province"},{"name":"Shuangchuang Project of Jiangsu Province"},{"name":"333 projects of Jiangsu Province","award":["BRA2017542"],"award-info":[{"award-number":["BRA2017542"]}]},{"name":"Natural Science Foundation of the Jiangsu Higher Education Institutions of China","award":["18KJA320010"],"award-info":[{"award-number":["18KJA320010"]}]},{"name":"Jiangsu Province's Graduate Scientific Research Innovation Program","award":["KYCX22-2896"],"award-info":[{"award-number":["KYCX22-2896"]}]},{"name":"Jiangsu Province's Graduate Scientific Research Innovation Program","award":["KYCX21-2691"],"award-info":[{"award-number":["KYCX21-2691"]}]},{"name":"Youth Science and Technology Innovation Team of Xuzhou Medical University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:p>\n            Background\u2009Transforming growth factor-\u03b21 (TGF-\u03b21) modulates multiple cellular functions during development and tissue homeostasis. A large amount of TGF-\u03b21 is stored in platelet \u03b1-granules and released upon platelet activation. Whether platelet-derived TGF-\u03b21 plays a role in venous thrombosis remains unclear. This study intends to assess the role of platelet-derived TGF-\u03b21 in the development of venous thrombosis in mice.<\/jats:p><jats:p>\n            Material and Methods\u2009TGF-\u03b21flox\/flox and platelet-specific TGF-\u03b21\u2212\/\u2212 mice were utilized to assess platelet function in vitro, arterial thrombosis induced by FeCl3, tail bleeding time, prothrombin time (PT), activated partial thromboplastin time (APTT), and deep vein thrombosis induced through ligation of the inferior vena cava (IVC). The IVC sample was collected to measure accumulation of neutrophils, monocytes, and the formation of neutrophil extracellular traps (NETs) by immunofluorescence staining.<\/jats:p><jats:p>\n            Results\u2009TGF-\u03b21 deficiency in platelets did not affect the number of circulating platelets, platelet aggregation, adenosine triphosphate release, and integrin \u03b1IIb\u03b23 activation. Meanwhile, TGF-\u03b21 deficiency did not alter the arterial thrombus formation, hemostasis, and coagulation time (PT and APTT), but significantly impaired venous thrombus formation, inhibited the recruitment and accumulation of neutrophils and monocytes in thrombi, as well as reduced formation of NETs and platelet-neutrophil complex. In addition, adoptive transfer of TGF-\u03b21flox\/flox platelets to TGF-\u03b21\u2212\/\u2212 mice rescued the impaired venous thrombus formation, recruitment of leukocytes and monocytes, as well as the NETs formation.<\/jats:p><jats:p>\n            Conclusion In conclusion, platelet-derived TGF-\u03b21 positively modulates venous thrombus formation in mice, indicating that targeting TGF-\u03b21 might be a novel approach for treating venous thrombosis without increasing the risk of bleeding.<\/jats:p>","DOI":"10.1055\/a-2235-7485","type":"journal-article","created":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T23:39:16Z","timestamp":1703720356000},"page":"641-648","source":"Crossref","is-referenced-by-count":18,"title":["Platelet-Derived TGF-\u03b21 Promotes Deep Vein Thrombosis"],"prefix":"10.1055","volume":"124","author":[{"given":"Sixuan","family":"Zhang","sequence":"first","affiliation":[{"name":"Blood Diseases Institute, Xuzhou Medical University, Xuzhou, 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