{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T14:39:30Z","timestamp":1786631970207,"version":"build-2736575974"},"reference-count":46,"publisher":"Georg Thieme Verlag KG","issue":"07\/08","license":[{"start":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T00:00:00Z","timestamp":1717632000000},"content-version":"vor","delay-in-days":5,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Austrian Science Fund","award":["P31915"],"award-info":[{"award-number":["P31915"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Antimicrobial photodynamic therapy (aPDT) is an evolving treatment strategy against human pathogenic microbes such as the Candida species, including the emerging pathogen C.\u00a0auris. Using a modified EUCAST protocol, the light-enhanced antifungal activity of the natural compound parietin was explored. The photoactivity was evaluated against three separate strains of five yeasts, and its molecular mode of action was analysed via several techniques, i.e., cellular uptake, reactive electrophilic species (RES), and singlet oxygen yield. Under experimental conditions (\u03bb\u2009=\u2009428\u2009nm, H\u2009=\u200930\u2009J\/cm2, PI\u2009=\u200930\u2009min), microbial growth was inhibited by more than 90% at parietin concentrations as low as c\u2009=\u20090.156\u2009mg\/L (0.55\u2009\u00b5M) for C. tropicalis and Cryptococcus neoformans, c\u2009=\u20090.313\u2009mg\/L (1.10\u2009\u00b5M) for C. auris, c\u2009=\u20090.625\u2009mg\/L (2.20\u2009\u00b5M) for C. glabrata, and c\u2009=\u20091.250\u2009mg\/L (4.40\u2009\u00b5M) for C.\u00a0albicans. Mode-of-action analysis\n          demonstrated fungicidal activity. Parietin targets the cell membrane and induces cell death via ROS-mediated lipid peroxidation after light irradiation. In summary, parietin exhibits light-enhanced fungicidal activity against all Candida species tested (including C. auris) and Cryptococcus neoformans, covering three of the four critical threats on the WHO\u02bcs most recent fungal priority list.<\/jats:p>","DOI":"10.1055\/a-2249-9110","type":"journal-article","created":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T22:34:57Z","timestamp":1717713297000},"page":"588-594","source":"Crossref","is-referenced-by-count":7,"title":["The Light-activated Effect of Natural Anthraquinone Parietin against Candida auris and Other Fungal Priority Pathogens"],"prefix":"10.1055","volume":"90","author":[{"given":"Johannes","family":"Fiala","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Institute of Pharmacy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Roach","sequence":"additional","affiliation":[{"name":"Department of Botany, University of Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Holzinger","sequence":"additional","affiliation":[{"name":"Department of Botany, University of Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yurii","family":"Husiev","sequence":"additional","affiliation":[{"name":"Leiden Institute of Chemistry, Leiden University, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Delueg","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Institute of Pharmacy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian","family":"Hammerle","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Institute of Pharmacy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva Sanchez","family":"Armengol","sequence":"additional","affiliation":[{"name":"Department of Technology, Institute of Pharmacy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald","family":"Sch\u00f6bel","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, MCI Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvestre","family":"Bonnet","sequence":"additional","affiliation":[{"name":"Leiden Institute of Chemistry, Leiden University, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia","family":"Laffleur","sequence":"additional","affiliation":[{"name":"Department of Technology, Institute of Pharmacy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilse","family":"Kranner","sequence":"additional","affiliation":[{"name":"Department of Botany, University of Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Lackner","sequence":"additional","affiliation":[{"name":"Institute of Hygiene und Medical Microbiology, Medical University of Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4910-1756","authenticated-orcid":false,"given":"Bianka","family":"Siewert","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Institute of Pharmacy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2024,6,6]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1111\/j.1439-0507.2009.01691.x","article-title":"The changing face of epidemiology of invasive fungal disease in Europe","volume":"52","author":"C Lass-Fl\u00f6rl","year":"2009","journal-title":"Mycoses"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1111\/j.1574-6976.2011.00278.x","article-title":"Candida glabrata, Candida parapsilosis and Candida tropicalis: biology, epidemiology, pathogenicity and antifungal resistance","volume":"36","author":"S Silva","year":"2012","journal-title":"FEMS Microbiol Rev"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"e1008921","DOI":"10.1371\/journal.ppat.1008921","article-title":"Candida auris: Epidemiology, biology, antifungal resistance, and 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