{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:17:46Z","timestamp":1791940666781,"version":"4.3.4"},"reference-count":84,"publisher":"Georg Thieme Verlag KG","issue":"08","funder":[{"DOI":"10.13039\/100000016","name":"U.S. Department of Health and Human Services","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000016","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"crossref","award":["HL144552"],"award-info":[{"award-number":["HL144552"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"crossref","award":["HL157975-01A1"],"award-info":[{"award-number":["HL157975-01A1"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"crossref","award":["HL164016-01A1"],"award-info":[{"award-number":["HL164016-01A1"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:p>\n            Background\u2003Neutrophil NETosis and neutrophil extracellular traps (NETs) play a critical role in pathogenesis of coronavirus disease 2019 (COVID-19)-associated thrombosis. However, the extents and reserve of NETosis, and potential of thrombus formation under shear in whole blood of patients with COVID-19 are not fully elucidated. Neither has the role of recombinant ADAMTS13 or caplacizumab on the accumulation of NETs and thrombus in COVID-19 patients' whole blood under shear been investigated.<\/jats:p><jats:p>\n            Methods\u2003Flow cytometry and microfluidic assay, as well as immunoassays, were employed for the study.<\/jats:p><jats:p>\n            Results\u2003We demonstrated that the percentage of H3Cit\u2009+\u2009MPO+ neutrophils, indicative of NETosis, was dramatically increased in patients with severe but not critical COVID-19 compared with that in asymptomatic or mild disease controls. Upon stimulation with poly [I:C], a double strain DNA mimicking viral infection, or bacterial shigatoxin-2, the percentage of H3Cit\u2009+\u2009MPO+ neutrophils was not significantly increased in the whole blood of severe and critical COVID-19 patients compared with that of asymptomatic controls, suggesting the reduction in NETosis reserve in these patients. Microfluidic assay demonstrated that the accumulation of NETs and thrombus was significantly enhanced in the whole blood of severe\/critical COVID-19 patients compared with that of asymptomatic controls. Like DNase I, recombinant ADAMTS13 or caplacizumab dramatically reduced the NETs accumulation and thrombus formation under arterial shear.<\/jats:p><jats:p>\n            Conclusion\u2003Significantly increased neutrophil NETosis, reduced NETosis reserve, and enhanced thrombus formation under arterial shear may play a crucial role in the pathogenesis of COVID-19-associated coagulopathy. Recombinant ADAMTS13 or caplacizumab may be explored for the treatment of COVID-19-associated thrombosis.<\/jats:p>","DOI":"10.1055\/a-2253-9359","type":"journal-article","created":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T23:43:30Z","timestamp":1706226210000},"page":"725-738","source":"Crossref","is-referenced-by-count":14,"title":["ADAMTS13 or Caplacizumab Reduces the Accumulation of Neutrophil Extracellular Traps and Thrombus in Whole Blood of COVID-19 Patients under Flow"],"prefix":"10.1055","volume":"124","author":[{"given":"Noritaka","family":"Yada","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, The University of Kansas Medical Center, Kanas City, Kansas, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, The University of Kansas Medical Center, Kanas City, Kansas, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia","family":"Bignotti","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, The University of Kansas Medical Center, Kanas City, Kansas, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhan","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, The University of Kansas Medical Center, Kanas City, Kansas, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1680-5295","authenticated-orcid":false,"given":"X. Long","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, The University of Kansas Medical Center, Kanas City, Kansas, United States"},{"name":"Institute of Reproductive Medicine and Developmental Sciences, The University of Kansas Medical Center, Kanas City, Kansas, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2024,3,5]]},"reference":[{"issue":"08","key":"ref1","doi-asserted-by":"crossref","first-page":"1995","DOI":"10.1111\/jth.14888","article-title":"Incidence of venous thromboembolism in hospitalized patients with COVID-19","volume":"18","author":"S Middeldorp","year":"2020","journal-title":"J Thromb Haemost"},{"issue":"06","key":"ref2","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.1111\/jth.14842","article-title":"Venous thromboembolism in COVID-19 patients","volume":"18","author":"A Porfidia","year":"2020","journal-title":"J Thromb 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Dong","year":"2002","journal-title":"Blood"},{"issue":"22","key":"ref31","doi-asserted-by":"crossref","first-page":"1578","DOI":"10.1056\/NEJM199811263392202","article-title":"von Willebrand factor-cleaving protease in thrombotic thrombocytopenic purpura and the hemolytic-uremic syndrome","volume":"339","author":"M Furlan","year":"1998","journal-title":"N Engl J Med"},{"issue":"22","key":"ref32","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.1056\/NEJM199811263392203","article-title":"Antibodies to von Willebrand factor-cleaving protease in acute thrombotic thrombocytopenic purpura","volume":"339","author":"H M Tsai","year":"1998","journal-title":"N Engl J Med"},{"issue":"06","key":"ref33","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1111\/bjh.17216","article-title":"von Willebrand factor\/ADAMTS13 axis and venous thromboembolism in moderate-to-severe COVID-19 patients","volume":"192","author":"M Delrue","year":"2021","journal-title":"Br J Haematol"},{"issue":"S 01","key":"ref34","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1111\/ijlh.13415","article-title":"ADAMTS13 activity to von Willebrand factor antigen ratio predicts acute kidney injury in patients with COVID-19: Evidence of SARS-CoV-2 induced secondary thrombotic microangiopathy","volume":"43","author":"B M Henry","year":"2021","journal-title":"Int J Lab Hematol"},{"issue":"04","key":"ref35","doi-asserted-by":"crossref","first-page":"O152","DOI":"10.1111\/ijlh.13476","article-title":"Increased von Willebrand factor antigen and low ADAMTS13 activity are related to poor prognosis in covid-19 patients","volume":"43","author":"M Rodr\u00edguez Rodr\u00edguez","year":"2021","journal-title":"Int J Lab Hematol"},{"issue":"01","key":"ref36","doi-asserted-by":"crossref","first-page":"e89","DOI":"10.1055\/s-0041-1723784","article-title":"Low ADAMTS13 activity correlates with increased mortality in COVID-19 patients","volume":"5","author":"J M Sweeney","year":"2021","journal-title":"TH Open"},{"issue":"01","key":"ref37","doi-asserted-by":"crossref","first-page":"34","DOI":"10.5858\/arpa.2021-0255-SA","article-title":"Elevated von Willebrand factor antigen is an early predictor of mortality and prolonged length of stay for coronavirus disease 2019 (COVID-19) inpatients","volume":"146","author":"A H Cotter","year":"2022","journal-title":"Arch Pathol Lab Med"},{"issue":"09","key":"ref38","doi-asserted-by":"crossref","first-page":"2193","DOI":"10.1111\/jth.15445","article-title":"Imbalance of von Willebrand factor and ADAMTS13 axis is rather a biomarker of strong inflammation and endothelial damage than a cause of thrombotic process in critically ill COVID-19 patients","volume":"19","author":"B S Joly","year":"2021","journal-title":"J Thromb Haemost"},{"issue":"08","key":"ref39","doi-asserted-by":"crossref","first-page":"1914","DOI":"10.1111\/jth.15409","article-title":"ADAMTS13 regulation of VWF multimer distribution in severe COVID-19","volume":"19","author":"S E Ward","year":"2021","journal-title":"J Thromb Haemost"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1160\/TH14-05-0455","article-title":"Neutrophil extracellular traps (NETs) and the role of platelets in infection","volume":"112","author":"R K Andrews","year":"2014","journal-title":"Thromb Haemost"},{"issue":"04","key":"ref41","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1084\/jem.20112322","article-title":"Monocytes, neutrophils, and platelets cooperate to initiate and propagate venous thrombosis in mice in vivo","volume":"209","author":"M L von Br\u00fchl","year":"2012","journal-title":"J Exp Med"},{"issue":"03","key":"ref42","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1111\/j.1538-7836.2007.02865.x","article-title":"Platelets, neutrophils, and neutrophil extracellular traps (NETs) in sepsis","volume":"6","author":"A C Ma","year":"2008","journal-title":"J Thromb Haemost"},{"issue":"02","key":"ref43","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.arbres.2022.10.004","article-title":"Response to aspirin therapy in COVID-19: prevention of NETosis","volume":"59","author":"A Lal","year":"2023","journal-title":"Arch Bronconeumol"},{"issue":"01","key":"ref44","first-page":"e14","article-title":"Effect of ticagrelor compared to clopidogrel on short-term outcomes of COVID-19 patients with acute coronary syndrome undergoing percutaneous coronary intervention; a randomized clinical trial","volume":"11","author":"R Arefizadeh","year":"2023","journal-title":"Arch Acad Emerg Med"},{"issue":"10","key":"ref45","doi-asserted-by":"crossref","first-page":"2261","DOI":"10.1161\/ATVBAHA.111.229609","article-title":"Essential domains of a disintegrin and metalloprotease with thrombospondin type 1 repeats-13 metalloprotease required for modulation of arterial thrombosis","volume":"31","author":"J Xiao","year":"2011","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"25","key":"ref46","doi-asserted-by":"crossref","first-page":"2497","DOI":"10.1056\/NEJMc1603180","article-title":"Caplacizumab for acquired thrombotic thrombocytopenic purpura","volume":"374","author":"F Peyvandi","year":"2016","journal-title":"N Engl J Med"},{"issue":"15","key":"ref47","doi-asserted-by":"crossref","first-page":"1639","DOI":"10.1007\/s40265-018-0989-0","article-title":"Caplacizumab: first global approval","volume":"78","author":"S Duggan","year":"2018","journal-title":"Drugs"},{"issue":"02","key":"ref48","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1111\/jth.15176","article-title":"Plasma levels of S100A8\/A9, histone\/DNA complexes, and cell-free DNA predict adverse outcomes of immune thrombotic thrombocytopenic purpura","volume":"19","author":"J Sui","year":"2021","journal-title":"J Thromb Haemost"},{"issue":"04","key":"ref49","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1160\/TH16-11-0834","article-title":"Plasma ADAMTS13 activity and von Willebrand factor antigen and activity in patients with subarachnoid haemorrhage","volume":"117","author":"M Kumar","year":"2017","journal-title":"Thromb Haemost"},{"issue":"11","key":"ref50","doi-asserted-by":"crossref","first-page":"2731","DOI":"10.1161\/ATVBAHA.118.311407","article-title":"Transfusion of platelets loaded with recombinant ADAMTS13 (a disintegrin and metalloprotease with thrombospondin type 1 repeats-13) is efficacious for inhibiting arterial thrombosis associated with thrombotic thrombocytopenic purpura","volume":"38","author":"M S Abdelgawwad","year":"2018","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"04","key":"ref51","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1111\/jth.14407","article-title":"Human neutrophil peptide-1 inhibits thrombus formation under arterial flow via 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J"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1007\/978-1-0716-0154-9_25","article-title":"Detection, visualization, and quantification of neutrophil extracellular traps (NETs) and NET markers","volume":"2087","author":"N de Buhr","year":"2020","journal-title":"Methods Mol Biol"},{"issue":"01","key":"ref56","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1055\/s-0038-1677040","article-title":"Neutrophil extracellular traps in arterial and venous thrombosis","volume":"45","author":"E Laridan","year":"2019","journal-title":"Semin Thromb Hemost"},{"issue":"18","key":"ref57","doi-asserted-by":"crossref","first-page":"2768","DOI":"10.1182\/blood-2013-10-463646","article-title":"Thrombosis: tangled up in NETs","volume":"123","author":"K Martinod","year":"2014","journal-title":"Blood"},{"issue":"01","key":"ref58","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1111\/j.1538-7836.2011.04544.x","article-title":"Neutrophil extracellular traps promote deep vein thrombosis in mice","volume":"10","author":"A Brill","year":"2012","journal-title":"J Thromb Haemost"},{"issue":"08","key":"ref59","doi-asserted-by":"crossref","first-page":"1777","DOI":"10.1161\/ATVBAHA.111.242859","article-title":"Neutrophil extracellular trap (NET) impact on deep vein thrombosis","volume":"32","author":"T A Fuchs","year":"2012","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"36","key":"ref60","doi-asserted-by":"crossref","first-page":"15880","DOI":"10.1073\/pnas.1005743107","article-title":"Extracellular DNA traps promote thrombosis","volume":"107","author":"T A Fuchs","year":"2010","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1146\/annurev.immunol.23.021704.115653","article-title":"How neutrophils kill microbes","volume":"23","author":"A W Segal","year":"2005","journal-title":"Annu Rev Immunol"},{"issue":"04","key":"ref62","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.1172\/JCI112120","article-title":"Defensins. Natural peptide antibiotics of human neutrophils","volume":"76","author":"T Ganz","year":"1985","journal-title":"J Clin Invest"},{"issue":"01","key":"ref63","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1111\/j.1600-065X.2011.01082.x","article-title":"\u03b1-Defensins in human innate immunity","volume":"245","author":"R I Lehrer","year":"2012","journal-title":"Immunol Rev"},{"issue":"06","key":"ref64","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1182\/blood-2012-02-412197","article-title":"Circulating DNA and myeloperoxidase indicate disease activity in patients with thrombotic microangiopathies","volume":"120","author":"T A Fuchs","year":"2012","journal-title":"Blood"},{"issue":"03","key":"ref65","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1083\/jcb.201006052","article-title":"Neutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps","volume":"191","author":"V Papayannopoulos","year":"2010","journal-title":"J Cell Biol"},{"key":"ref66","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1007\/978-1-4899-0117-0_24","article-title":"Myeloperoxidase (MPO) may mediate neutrophil adherence to the endothelium through upregulation of CD11B expression\u2013an effect downregulated by taurine","volume":"442","author":"P P Stapleton","year":"1998","journal-title":"Adv Exp Med Biol"},{"issue":"11","key":"ref67","doi-asserted-by":"crossref","first-page":"2990","DOI":"10.1002\/art.39247","article-title":"Release of neutrophil extracellular traps by neutrophils stimulated with antiphospholipid antibodies: a newly identified mechanism of thrombosis in the antiphospholipid syndrome","volume":"67","author":"S Yalavarthi","year":"2015","journal-title":"Arthritis Rheumatol"},{"key":"ref68","doi-asserted-by":"crossref","first-page":"1078891","DOI":"10.3389\/fimmu.2022.1078891","article-title":"Impact of neutrophil extracellular traps on fluid properties, blood flow and complement activation","volume":"13","author":"A Burmeister","year":"2022","journal-title":"Front Immunol"},{"issue":"01","key":"ref69","first-page":"67","article-title":"PAMPs and DAMPs as triggers for DIC","volume":"2","author":"T Ito","year":"2014","journal-title":"J Intensive Care"},{"issue":"01","key":"ref70","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1038\/nri3345","article-title":"Thrombosis as an intravascular effector of innate immunity","volume":"13","author":"B Engelmann","year":"2013","journal-title":"Nat Rev Immunol"},{"issue":"12","key":"ref71","first-page":"9","article-title":"Shiga toxin 2a induces NETosis via NOX-dependent pathway","volume":"9","author":"W JC Feitz","year":"2021","journal-title":"Biomedicines"},{"issue":"04","key":"ref72","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1159\/000445770","article-title":"Induction of neutrophil extracellular traps in shiga toxin-associated hemolytic uremic syndrome","volume":"8","author":"M V Ramos","year":"2016","journal-title":"J Innate Immun"},{"issue":"01","key":"ref73","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.cellimm.2010.10.008","article-title":"Novel antagonist antibody to TLR3 blocks poly(I:C)-induced inflammation in vivo and in vitro","volume":"267","author":"R A Bunting","year":"2011","journal-title":"Cell Immunol"},{"issue":"04","key":"ref74","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1007\/s11259-021-09809-z","article-title":"Co-administration of toll-like receptor (TLR)-3 agonist Poly I:C with different infectious bursal disease (IBD) vaccines improves IBD specific immune response in chicken","volume":"45","author":"T R Kannaki","year":"2021","journal-title":"Vet Res Commun"},{"issue":"01","key":"ref75","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1186\/1465-9921-10-43","article-title":"Long-term activation of TLR3 by poly(I:C) induces inflammation and impairs lung function in mice","volume":"10","author":"N C Stowell","year":"2009","journal-title":"Respir Res"},{"key":"ref76","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1182\/blood-2021-153272","article-title":"Neutrophil extracellular traps (NETs) contribute to the formation of microvascular thrombosis in immune thrombotic thrombocytopenic purpura","volume":"138","author":"N Yada","year":"2021","journal-title":"Blood"},{"issue":"09","key":"ref77","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.1161\/ATVBAHA.114.304114","article-title":"Neutrophil extracellular traps promote thrombin generation through platelet-dependent and platelet-independent mechanisms","volume":"34","author":"T J Gould","year":"2014","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"05","key":"ref78","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1038\/nm.3547","article-title":"Aggregated neutrophil extracellular traps limit inflammation by degrading cytokines and chemokines","volume":"20","author":"C Schauer","year":"2014","journal-title":"Nat Med"},{"issue":"6367","key":"ref79","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1126\/science.aam8897","article-title":"Host DNases prevent vascular occlusion by neutrophil extracellular traps","volume":"358","author":"M Jim\u00e9nez-Alc\u00e1zar","year":"2017","journal-title":"Science"},{"issue":"01","key":"ref80","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1186\/s13075-015-0726-y","article-title":"A subset of patients with systemic lupus erythematosus fails to degrade DNA from multiple clinically relevant sources","volume":"17","author":"J Leffler","year":"2015","journal-title":"Arthritis Res Ther"},{"issue":"05","key":"ref81","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1111\/jth.12796","article-title":"Impaired DNase1-mediated degradation of neutrophil extracellular traps is associated with acute thrombotic microangiopathies","volume":"13","author":"M 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