{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T12:23:10Z","timestamp":1772281390860,"version":"3.50.1"},"reference-count":40,"publisher":"Georg Thieme Verlag KG","issue":"05","funder":[{"DOI":"10.13039\/100009086","name":"Medical University Sofia","doi-asserted-by":"crossref","award":["D-117\/24.06.2020"],"award-info":[{"award-number":["D-117\/24.06.2020"]}],"id":[{"id":"10.13039\/100009086","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Exp Clin Endocrinol Diabetes"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Purpose: This preliminary study aimed to analyze and identify\n                    differentially expressed miRNAs in Bulgarian patients with non-functioning\n                    pituitary neuroendocrine tumors (NFPitNET). The relationship between deregulated\n                    miRNAs and tumor invasiveness, recurrence, and size was determined.<\/jats:p><jats:p>\n          Methods: Twenty patients with NFPitNET were selected and fresh pituitary\n                    tumor tissues were collected. RNA containing miRNAs were isolated using\n                    miRNAeasy mini kit and analyzed by quantitative real-time polymerase chain\n                    reaction (PCR) using LNA miRNA Cancer-Focus PCR Panel\n                    (Qiagen).<\/jats:p><jats:p>\n          Results: Three miRNAs (miR-210-3p, miR-149-3p, and miR-29b-3p) were\n                    deregulated in invasive compared to non-invasive NFPitNETs. Differential\n                    expression of four-miRNA signatures \u2013 miRNA-17, miR-19, miR-106a, and miR-20,\n                    correlated with patient recurrence.<\/jats:p><jats:p>\n          Conclusion: This prospective pilot study selected a unique miRNA\n                    expression profile, that correlates with invasiveness and recurrence in\n                    non-functioning pituitary neuroendocrine tumors. Moreover, some of the selected\n                    miRNAs are reported for the first time in patients with this disease, shedding\n                    light on the molecular mechanisms involved in pituitary pathogenesis. The\n                    identified miRNAs demonstrate potential as biomarkers, deserving further\n                    investigation in a larger cohort to validate their clinical applicability.<\/jats:p>","DOI":"10.1055\/a-2268-4129","type":"journal-article","created":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T00:08:55Z","timestamp":1707955735000},"page":"240-248","source":"Crossref","is-referenced-by-count":3,"title":["MiRNA Signatures Related to Invasiveness and Recurrence in Patients\n                    With Non-Functioning Pituitary Neuroendocrine Tumors"],"prefix":"10.1055","volume":"132","author":[{"given":"Emiliya","family":"Nikolova","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry and Biochemistry, Medical\n                    University-Sofia, Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anelia","family":"Nankova","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Medical University-Sofia, USHATE \u201cAcad.\n                    Ivan Penchev\u201d, Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Kalenderova","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry and Biochemistry, Medical\n                    University-Sofia, Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bilyana","family":"Georgieva","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry and Biochemistry, Medical\n                    University-Sofia, Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asen","family":"Hadzhiyanev","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Medical University-Sofia, University\n                    Hospital \u201cSt. Ivan Rilski\u201d Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stoyan","family":"Bichev","sequence":"additional","affiliation":[{"name":"National Genetic Laboratory, Medical University- Sofia, UHOG \u201cMaichin\n                    dom\u201d, Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexey","family":"Savov","sequence":"additional","affiliation":[{"name":"National Genetic Laboratory, Medical University- Sofia, UHOG \u201cMaichin\n                    dom\u201d, Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albena","family":"Todorova","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry and Biochemistry, Medical\n                    University-Sofia, Sofia, Bulgaria"},{"name":"Genetic and Medico-diagnostic Laboratory \u201cGenica\u201d, Sofia,\n                    Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanyo","family":"Mitev","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry and Biochemistry, Medical\n                    University-Sofia, Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9821-4952","authenticated-orcid":false,"given":"Atanaska","family":"Elenkova","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Medical University-Sofia, USHATE \u201cAcad.\n                    Ivan Penchev\u201d, Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2024,4,15]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1002\/cncr.20412","article-title":"The prevalence of pituitary adenomas: A systematic review","volume":"101","author":"S Ezzat","year":"2004","journal-title":"Cancer"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.ando.2015.04.002","article-title":"Management of clinically non-functioning pituitary adenoma","volume":"76","author":"P Chanson","year":"2015","journal-title":"Ann Endocrinol (Paris)"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"G1","DOI":"10.1530\/EJE-17-0796","article-title":"European Society of Endocrinology Clinical Practice Guidelines for the\n                    management of aggressive pituitary tumours and carcinomas","volume":"178","author":"G Raverot","year":"2018","journal-title":"Eur J Endocrinol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1210\/jc.2005-2552","article-title":"Observation alone after transsphenoidal surgery for nonfunctioning pituitary\n                    macroadenoma","volume":"91","author":"O M Dekkers","year":"2006","journal-title":"J Clin Endocrinol Metab"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"E7","DOI":"10.3171\/foc.2004.16.4.8","article-title":"Pituitary carcinoma: A review of the literature","volume":"16","author":"B T Ragel","year":"2004","journal-title":"Neurosurg Focus"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1038\/ncpendmet0159","article-title":"Mechanisms of disease: The pathogenesis of pituitary tumors","volume":"2","author":"S Ezzat","year":"2006","journal-title":"Nat Clin Pract Endocrinol Metab"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1007\/s11102-015-0648-3","article-title":"MicroRNA involvement in a metastatic non-functioning pituitary carcinoma","volume":"18","author":"Z Wei","year":"2015","journal-title":"Pituitary"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.cell.2018.03.006","article-title":"Metazoan MicroRNAs","volume":"173","author":"D P Bartel","year":"2018","journal-title":"Cell"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1546","DOI":"10.1136\/gut.2009.179531","article-title":"MicroRNAs: Tools for cancer diagnostics","volume":"58","author":"T Paranjape","year":"2009","journal-title":"Gut"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ydbio.2006.08.028","article-title":"microRNAs as oncogenes and tumor suppressors","volume":"302","author":"B Zhang","year":"2007","journal-title":"Dev Biol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1016\/j.bbrc.2005.07.030","article-title":"Extensive modulation of a set of microRNAs in primary glioblastoma","volume":"334","author":"S A Ciafr\u00e8","year":"2005","journal-title":"Biochem Biophys Res Commun"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1007\/BF03346759","article-title":"MicroRNAs: Suggested role in pituitary adenoma pathogenesis","volume":"36","author":"M Gadelha","year":"2013","journal-title":"J Endocrinol Invest"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1038\/nrendo.2011.40","article-title":"Pathogenesis of pituitary tumors","volume":"7","author":"S Melmed","year":"2011","journal-title":"Nat Rev Endocrinol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1227\/NEU.0000000000000369","article-title":"MicroRNAs as biomarkers in pituitary tumors","volume":"75","author":"A Di Ieva","year":"2014","journal-title":"Neurosurgery"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1007\/s11102-010-0268-x","article-title":"MicroRNA profile indicates downregulation of the TGF\u03b2 pathway in sporadic\n                    non-functioning pituitary adenomas","volume":"14","author":"H Butz","year":"2011","journal-title":"Pituitary"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1007\/s10528-017-9794-9","article-title":"Novel biomarkers for non-functioning invasive pituitary adenomas were identified\n                    by using analysis of microRNAs expression profile","volume":"55","author":"S Wu","year":"2017","journal-title":"Biochem Genet"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1007\/s11102-012-0454-0","article-title":"Prevalence and incidence of pituitary adenomas: A population based study in\n                    Malta","volume":"16","author":"M Gruppetta","year":"2013","journal-title":"Pituitary"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"20881","DOI":"10.18632\/oncotarget.14930","article-title":"microRNA-210-3p depletion by CRISPR\/Cas9 promoted tumorigenesis through revival\n                    of TWIST1 in renal cell carcinoma","volume":"8","author":"H Yoshino","year":"2017","journal-title":"Oncotarget"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1007\/s40618-019-01178-4","article-title":"MicroRNAs expression in pituitary tumors: Differences related to functional\n                    status, pathological features, and clinical behavior","volume":"43","author":"T M Vicchio","year":"2020","journal-title":"J Endocrinol Invest"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1007\/s10565-020-09570-0","article-title":"Up-regulation of the expressions of MiR-149-5p and MiR-99a-3p in exosome\n                    inhibits the progress of pituitary adenomas","volume":"37","author":"P Zhao","year":"2021","journal-title":"Cell Biol Toxicol"},{"key":"ref21","first-page":"539","article-title":"The role of miR-29b in cancer: Regulation, function, and signaling","volume":"8","author":"B Yan","year":"2015","journal-title":"Onco Targets Ther"},{"key":"ref22","first-page":"1","article-title":"MicroRNA-29-3p regulates the \u03b2-catenin pathway by targeting IGF1 to inhibit the\n                    proliferation of prolactinoma cells","volume":"23","author":"J Xia","year":"2021","journal-title":"Mol Med Rep"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/diagnostics10040227","article-title":"Increased E2F1 mRNA and miR-17-5p expression is correlated to invasiveness and\n                    proliferation of pituitary neuroendocrine tumours","volume":"10","author":"A Garc\u00eda-Mart\u00ednez","year":"2020","journal-title":"Diagnostics"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"2589","DOI":"10.3892\/ijo.2016.3746","article-title":"MiR-19a correlates with poor prognosis of clear cell renal cell carcinoma\n                    patients via promoting cell proliferation and suppressing PTEN\/SMAD4\n                    expression","volume":"49","author":"Q Ma","year":"2016","journal-title":"Int J Oncol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"4784500","DOI":"10.1155\/2023\/4784500","article-title":"miR-19-3p targets PTEN to regulate cervical cancer cell proliferation, invasion,\n                    and autophagy","volume":"2023","author":"W Wang","year":"2023","journal-title":"Genet Res (Camb)"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"4564","DOI":"10.3748\/wjg.v21.i15.4564","article-title":"MiR-19a promotes epithelial-mesenchymal transition through PI3K\/AKT pathway in\n                    gastric cancer","volume":"21","author":"W D Lu","year":"2015","journal-title":"World J Gastroenterol"},{"key":"ref27","first-page":"2491","article-title":"MiR-16 regulates proliferation and apoptosis of pituitary adenoma cells by\n                    inhibiting HMGA2","volume":"17","author":"Y Niu","year":"2019","journal-title":"Oncol Lett"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1038\/s41398-018-0224-5","article-title":"MiR-9, miR-153 and miR-124 are down-regulated by acute exposure to cocaine in a\n                    dopaminergic cell model and may contribute to cocaine dependence","volume":"8","author":"J Cabana-Dom\u00ednguez","year":"2018","journal-title":"Transl Psychiatry"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1530\/JOE-17-0135","article-title":"MicroRNA-9 regulates fetal alcohol-induced changes in D2 receptor to promote\n                    prolactin production","volume":"235","author":"O Gangisetty","year":"2017","journal-title":"J Endocrinol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"171","DOI":"10.4196\/kjpp.2019.23.3.171","article-title":"MicroRNA-186 targets SKP2 to induce p27Kip1-mediated pituitary tumor cell cycle\n                    deregulation and modulate cell proliferation","volume":"23","author":"Z He","year":"2019","journal-title":"Korean J Physiol Pharmacol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"4151","DOI":"10.1210\/jc.2018-02479","article-title":"Comprehensive analysis of circulating miRNAs in the plasma of patients with\n                    pituitary adenomas","volume":"104","author":"K N\u00e9meth","year":"2019","journal-title":"J Clin Endocrinol Metab"},{"key":"ref32","first-page":"1235","article-title":"MicroRNA-16\/VEGFR2\/p38\/NF-KB signaling pathway regulates cell growth of human\n                    pituitary neoplasms","volume":"39","author":"B Lu","year":"2018","journal-title":"Oncol Rep"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fendo.2020.00331","article-title":"Circulating plasma microRNA to differentiate Cushing\u2019s disease from ectopic ACTH\n                    syndrome","volume":"11","author":"Z Belaya","year":"2020","journal-title":"Front Endocrinol (Lausanne)"},{"key":"ref34","first-page":"2019","article-title":"Identification of serum miRNA-423-5p expression signature in somatotroph\n                    adenomas","author":"S Zhao","year":"2019","journal-title":"Int J Endocrinol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1186\/s12859-019-3105-x","article-title":"MIENTURNET: An interactive web tool for microRNA-target enrichment and\n                    network-based analysis","volume":"20","author":"V Licursi","year":"2019","journal-title":"BMC Bioinformatics"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1007\/s13167-019-00187-w","article-title":"TMT-based quantitative proteomics revealed follicle-stimulating hormone\n                    (FSH)-related molecular characterizations for potentially prognostic assessment\n                    and personalized treatment of FSH-positive non-functional pituitary\n                    adenomas","volume":"10","author":"Y Wang","year":"2019","journal-title":"EPMA J"},{"key":"ref37","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1055\/s-0029-1202832","article-title":"Platelet-derived growth factor (PDGF) and PDGF receptor expression and function\n                    in folliculostellate pituitary cells","volume":"118","author":"M Kowarik","year":"2010","journal-title":"Exp Clin Endocrinol Diabetes"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"373","DOI":"10.3390\/medicina59020373","article-title":"Expression and clinical significance of MDM2 in non-functioning PitNETs","volume":"59","author":"X Yao","year":"2023","journal-title":"Medicina (Kaunas)"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/s11060-016-2365-9","article-title":"Analysis of Ki67, HMGA1, MDM2, and RB expression in nonfunctioning pituitary\n                    adenomas","volume":"132","author":"X Yao","year":"2017","journal-title":"J Neuroonco"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"237","DOI":"10.3389\/fimmu.2018.00237","article-title":"Herpesviruses in the activated phosphatidylinositol-3-kinase-\u03b4 syndrome","volume":"9","author":"J I Cohen","year":"2018","journal-title":"Front Immunol"}],"container-title":["Experimental and Clinical Endocrinology &amp; Diabetes"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-2268-4129.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T11:32:19Z","timestamp":1730806339000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-2268-4129"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,14]]},"references-count":40,"journal-issue":{"issue":"05","published-online":{"date-parts":[[2024,5,2]]},"published-print":{"date-parts":[[2024,5]]}},"URL":"https:\/\/doi.org\/10.1055\/a-2268-4129","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["0947-7349","1439-3646"],"issn-type":[{"value":"0947-7349","type":"print"},{"value":"1439-3646","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024,2,14]]},"published":{"date-parts":[[2024,2,14]]}}}