{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T00:08:49Z","timestamp":1783728529101,"version":"3.55.0"},"reference-count":50,"publisher":"Georg Thieme Verlag KG","issue":"05","funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"crossref","award":["CB-2015-241044"],"award-info":[{"award-number":["CB-2015-241044"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"crossref","award":["IF-2015-254145"],"award-info":[{"award-number":["IF-2015-254145"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Agave applanata is a Mexican agave whose fresh leaves are employed to prepare an ethanol tonic used to relieve diabetes. It is also applied to skin to relieve varicose and diabetic foot ulcers, including wounds, inflammation, and infections. In this study, the chemical composition of this ethanol tonic is established and its association with antihyperglycemic, anti-inflammatory, antimicrobial, and wound healing activities is discussed. The fresh leaves of A.\u00a0applanata were extracted with ethanol\u200a:\u200aH2O (85\u200a:\u200a15). A fraction of this extract was lyophilized, and the remainder was partitioned into CH2Cl2, n-BuOH, and water. CH2Cl2 and n-BuOH fractions were subjected to a successive open column chromatography process. The structure of the isolated compounds was established using nuclear magnetic resonance and mass spectrometry spectra. The antihyperglycemic activity was evaluated through in\n          vivo sucrose and glucose tolerance experiments, as well as ex vivo intestinal absorption and hepatic production of glucose. Wound healing and edema inhibition were assayed in mice. The minimum inhibitory concentrations (MICs) of the hydroalcoholic extract, its fractions, and pure compounds were determined through agar microdilution against the most isolated pathogens from diabetic foot ulcers. Fatty acids, \u03b2-sitosterol, stigmasterol, hecogenin (1), N-oleyl-D-glucosamine, \u03b2-daucosterol, sucrose, myo-inositol, and hecogenin-3-O-\u03b1-L-rhamnopyranosyl-(1\u2009\u2192\u20093)-\u03b2-D-xylopyranosyl-(1\u2009\u2192\u20092)-[\u03b2-D-xylopyranosyl-(1\u2009\u2192\u20093)-\u03b2-D-glucopyranosyl-(1\u2009\u2192\u20093)]-\u03b2-D-glucopyranosyl-(1\u2009\u2192\u20094)-\u03b2-D-galactopyranoside (2) were characterized. This research provides evidence for the pharmacological importance of A.\u00a0applanata in maintaining normoglycemia, showing anti-inflammatory activity and antimicrobial\n          effects against the microorganisms frequently found in diabetic foot ulcers. This plant plays an important role in wound healing and accelerated tissue reparation.<\/jats:p>","DOI":"10.1055\/a-2270-5527","type":"journal-article","created":{"date-parts":[[2024,2,17]],"date-time":"2024-02-17T00:37:37Z","timestamp":1708130257000},"page":"397-410","source":"Crossref","is-referenced-by-count":3,"title":["Chemical Constituents from Agave applanata and Its Antihyperglycemic, Anti-inflammatory, and Antimicrobial Activities Associated with Its Tissue Repair Capability"],"prefix":"10.1055","volume":"90","author":[{"given":"A. Berenice","family":"Aguilar-Guadarrama","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Qu\u00edmicas-IICBA, Universidad Aut\u00f3noma del Estado de Morelos, Morelos, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nica Aide\u00e9","family":"D\u00edaz-Rom\u00e1n","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Qu\u00edmicas-IICBA, Universidad Aut\u00f3noma del Estado de Morelos, Morelos, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maribel","family":"Osorio-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Qu\u00edmicas-IICBA, Universidad Aut\u00f3noma del Estado de Morelos, Morelos, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myrna","family":"D\u00e9ciga-Campos","sequence":"additional","affiliation":[{"name":"Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Ciudad de M\u00e9xico, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8875-8734","authenticated-orcid":false,"given":"Mar\u00eda Yolanda","family":"Rios","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Qu\u00edmicas-IICBA, Universidad Aut\u00f3noma del Estado de Morelos, Morelos, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2024,3,12]]},"reference":[{"key":"ref3","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1111\/iwj.12932","article-title":"Diabetic foot infection: A critical complication","volume":"15","author":"J J Hurlow","year":"2018","journal-title":"Int Wound J"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1002\/micr.30753","article-title":"Lymphatico-venous anastomosis in chronic ulcer with venous insufficiency: A case report","volume":"41","author":"E 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