{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T17:58:42Z","timestamp":1782323922561,"version":"3.54.5"},"reference-count":54,"publisher":"Georg Thieme Verlag KG","issue":"04","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Z Geburtshilfe Neonatol"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The placenta is a temporary organ that provides communication between the mother\n                    and fetus. Maternal diabetes and abnormal placental angiogenesis may be linked.\n                    We investigated the angiogenesis mechanism resulting from VEGF and glucose\n                    stimulation in PECs obtained from human term placenta. Immunohistochemistry was\n                    performed to characterize PECs obtained from human term placenta. D-glucose was\n                    added to the medium containing PECs to establish normoglycemic and hyperglycemic\n                    conditions. The expression levels of VEGF, VEGFR-1 and VEGFR-2 genes and\n                    proteins in PECs from the control and experimental groups were analyzed by\n                    RT-PCR and Western blotting, respectively. With 48-hours incubation, gene\n                    expressions increased due to hyperglycemia, while protein levels increased due\n                    to the combined effect of VEGF and hyperglycemia. While VEGFR-2 gene expression\n                    and protein amounts increased in 24-hours due to the combined effect of VEGF and\n                    hyperglycemia, the effect of VEGF stimulation and glucose level on VEGFR-2\n                    decreased in 48-hour incubation with time. VEGF, VEGFR-1 and VEGFR-2 genes and\n                    proteins were affected by hyperglycemic conditions in PECs. Hyperglycemia\n                    occurring in various conditions such as gestational diabetes mellitus and\n                    diabetes mellitus may affect VEGF, VEGFR-1 and VEGFR-2 genes and proteins of\n                    PECs derived from human term placenta.<\/jats:p>","DOI":"10.1055\/a-2282-9007","type":"journal-article","created":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T23:21:30Z","timestamp":1715642490000},"page":"346-354","source":"Crossref","is-referenced-by-count":3,"title":["Effects of Hyperglycemia on Angiogenesis in Human Placental\n                    Endothelial Cells"],"prefix":"10.1055","volume":"228","author":[{"given":"Turkan","family":"Yanik","sequence":"additional","affiliation":[{"name":"Histology and Embryology, Akdeniz University Faculty of Medicine,\n                    Antalya, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ertan","family":"Katirci","sequence":"additional","affiliation":[{"name":"Histology and Embryology, Ahi Evran University Faculty of Medicine,\n                    Kirsehir, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet","family":"Simsek","sequence":"additional","affiliation":[{"name":"Obstetrics And Gynaecology, Akdeniz University Faculty of Medicine,\n                    Antalya, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emin Turkay","family":"Korgun","sequence":"additional","affiliation":[{"name":"Histology and Embryology, Akdeniz University Faculty of Medicine,\n                    Antalya, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dijle","family":"Kipmen-Korgun","sequence":"additional","affiliation":[{"name":"Department Of Medical Biochemistry, Akdeniz University Faculty of\n                    Medicine, Antalya, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2024,5,13]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"506","DOI":"10.1093\/jnci\/87.7.506","article-title":"Expression of vascular endothelial growth factor and its receptors flt and KDR\n                    in ovarian carcinoma","volume":"87","author":"C A Boocock","year":"1995","journal-title":"J Natl Cancer Inst"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1002\/(sici)1097-0215(20000101)85:1<27::aid-ijc5>3.0.co;2-8","article-title":"Concomitant over-expression of vascular endothelial growth factor and its\n                    receptors in pancreatic cancer","volume":"85","author":"J Itakura","year":"2000","journal-title":"Int J Cancer"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"410","DOI":"10.1002\/jlb.55.3.410","article-title":"Macrophages and angiogenesis","volume":"55","author":"C Sunderk\u00f6tter","year":"1994","journal-title":"J Leukoc Biol"},{"key":"ref4","first-page":"2187","article-title":"Platelet: transporter of vascular endothelial growth factor","volume":"3","author":"H M Verheul","year":"1997","journal-title":"Clin Cancer Res"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"12607","DOI":"10.1074\/jbc.270.21.12607","article-title":"Regulation of vascular endothelial growth factor expression in cultured\n                    keratinocytes. 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Chiarelli","year":"2000","journal-title":"Diabetes"},{"key":"ref33","doi-asserted-by":"publisher","first-page":"3093","DOI":"10.1681\/ASN.2006010064","article-title":"Blockade of vascular endothelial growth factor signaling ameliorates diabetic\n                    albuminuria in mice","volume":"17","author":"S H Sung","year":"2006","journal-title":"J Am Soc Nephrol"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1111\/j.1699-0463.1982.tb00069_90a.x","article-title":"Ultrastructure of the villi and fetal capillaries of the placentas delivered by\n                    non-smoking diabetic women (White group D)","volume":"90","author":"I Asmussen","year":"1982","journal-title":"Acta Pathol Microbiol Immunol Scand A"},{"key":"ref35","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1111\/j.1471-0528.1986.tb07809.x","article-title":"Quantitative structural studies on placentas from pregnancies complicated by\n                    diabetes mellitus","volume":"93","author":"P A Boyd","year":"1986","journal-title":"Br J Obstet Gynaecol"},{"key":"ref36","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1016\/s0143-4004(81)80007-0","article-title":"Histomorphometry of the placenta of the diabetic women: class A diabetes\n                    mellitus","volume":"2","author":"F Teasdale","year":"1981","journal-title":"Placenta"},{"key":"ref37","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1080\/00016340801908783","article-title":"Placental pathology in women with gestational diabetes","volume":"87","author":"G Daskalakis","year":"2008","journal-title":"Acta Obstet Gynecol Scand"},{"key":"ref38","doi-asserted-by":"publisher","first-page":"1434","DOI":"10.1007\/s00125-002-0927-1","article-title":"Enhanced fetoplacental angiogenesis in pre-gestational diabetes mellitus: the\n                    extra growth is exclusively longitudinal and not accompanied by microvascular\n                    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Lab Physicians"},{"key":"ref51","doi-asserted-by":"publisher","first-page":"22","DOI":"10.3390\/ijms222413378","article-title":"Transient hyperglycemia and hypoxia induce memory effects in angiomir expression\n                    profiles of feto-placental endothelial cells","author":"J Strutz","year":"2021","journal-title":"Int J Mol Sci"},{"key":"ref52","doi-asserted-by":"publisher","first-page":"S13","DOI":"10.2337\/dc19-S002","article-title":"2. Classification and Diagnosis of Diabetes: Standards of Medical Care in\n                    Diabetes\u20132019","volume":"42","author":"American Diabetes Association","year":"2018","journal-title":"Diabetes Care"},{"key":"ref53","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1186\/s13287-021-02192-1","article-title":"Conditioned medium from primary cytotrophoblasts, primary placenta-derived\n                    mesenchymal stem cells, or sub-cultured placental tissue promoted HUVEC\n                    angiogenesis in vitro","volume":"12","author":"H Ma","year":"2021","journal-title":"Stem Cell Res Ther"},{"key":"ref54","doi-asserted-by":"publisher","first-page":"1279227","DOI":"10.3389\/fcell.2023.1279227","article-title":"Exploring maternal-fetal interface with in vitro placental and trophoblastic\n                    models","volume":"11","author":"X Liu","year":"2023","journal-title":"Front Cell Dev Biol"}],"container-title":["Zeitschrift f\u00fcr Geburtshilfe und Neonatologie"],"original-title":[],"language":"de","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-2282-9007.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T13:49:27Z","timestamp":1730814567000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-2282-9007"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,13]]},"references-count":54,"journal-issue":{"issue":"04","published-online":{"date-parts":[[2024,8,5]]},"published-print":{"date-parts":[[2024,8]]}},"URL":"https:\/\/doi.org\/10.1055\/a-2282-9007","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["0948-2393","1439-1651"],"issn-type":[{"value":"0948-2393","type":"print"},{"value":"1439-1651","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,13]]}}}