{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T10:48:15Z","timestamp":1772016495786,"version":"3.50.1"},"reference-count":13,"publisher":"Georg Thieme Verlag KG","issue":"05","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hamostaseologie"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>During routine treatment, plasma samples of patients with hemophilia A or acquired hemophilia A are frequently analyzed for the presence of FVIII-specific antibodies. While only inhibitory antibodies can be detected by the Bethesda assay, inhibitory and non-inhibitory antibodies can be detected by ELISA. However, plasma samples of patients frequently contain endogenous or substituted FVIII, hence interfering with both types of analyses. One option for the inactivation of FVIII is heat denaturation, which unfortunately has been shown to lead to high background signals complicating the discrimination of negative and positive plasma samples. In the current study, we developed a method of acid denaturation for FVIII-containing plasma samples that can help identify samples containing FVIII-specific antibodies and compared the effects of heat and acid denaturation on the detection of FVIII\u2013antibody interactions in a monoclonal setting. The aim of our study was to establish an analysis that allows safer treatment decisions in the context of tolerance to FVIII.<\/jats:p>","DOI":"10.1055\/a-2329-1781","type":"journal-article","created":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T22:56:16Z","timestamp":1721084176000},"page":"350-357","source":"Crossref","is-referenced-by-count":2,"title":["Acid Treatment of FVIII-Containing Plasma Samples Unmasks a Broad Spectrum of FVIII-Specific Antibodies in ELISA"],"prefix":"10.1055","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8565-3964","authenticated-orcid":false,"given":"Anja","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Department of Paediatrics and Adolescent Medicine, Clinical and Molecular Haemostasis, Goethe University Frankfurt, Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana","family":"Stichel","sequence":"additional","affiliation":[{"name":"Department of Paediatrics and Adolescent Medicine, Clinical and Molecular Haemostasis, Goethe University Frankfurt, Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilia","family":"Salzmann-Manrique","sequence":"additional","affiliation":[{"name":"Department of Paediatrics and Adolescent Medicine, Clinical and Molecular Haemostasis, Goethe University Frankfurt, Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4597-2439","authenticated-orcid":false,"given":"Christoph","family":"K\u00f6nigs","sequence":"additional","affiliation":[{"name":"Department of Paediatrics and Adolescent Medicine, Clinical and Molecular Haemostasis, Goethe University Frankfurt, Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2024,7,15]]},"reference":[{"issue":"01","key":"ref1","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1056\/NEJM199401063300108","article-title":"Hemophilia A","volume":"330","author":"L W Hoyer","year":"1994","journal-title":"N Engl J Med"},{"issue":"06","key":"ref2","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1111\/j.1538-7836.2010.03823.x","article-title":"Rate of inhibitor development in previously untreated hemophilia A patients treated with plasma-derived or recombinant factor VIII concentrates: a systematic review","volume":"8","author":"A Iorio","year":"2010","journal-title":"J Thromb Haemost"},{"issue":"05","key":"ref3","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1111\/ijlh.12862","article-title":"Optimization of pre-analytical heat treatment for inhibitor detection in haemophilia A","volume":"40","author":"P 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