{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:33:29Z","timestamp":1740126809926,"version":"3.37.3"},"reference-count":18,"publisher":"Georg Thieme Verlag KG","issue":"01","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Perinatol"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n            Objective\u2003Mainstay therapy for fetuses affected by maternal red cell alloimmunization is serial intrauterine transfusion (IUT). Testing to determine when fetal red cells have been replaced with donor cells historically involves the use of the Kleihauer\u2013Betke (KB) test. Hemoglobin (Hgb) electrophoresis testing may be more rapid with a reduced cost of analysis. We aimed to determine the correlation between fetal Hgb electrophoresis versus the traditional KB test.<\/jats:p><jats:p>\n            Study Design\u2003This is a retrospective analysis of all alloimmunized singleton pregnancies undergoing IUT between January 1, 2021, and July 1, 2023. Maternal and fetal characteristics were collected along with the indication for IUT. A final fetal blood sample was obtained at the conclusion of each transfusion and sent for KB testing and Hgb electrophoresis. The primary outcome was the assessment of these parameters in their ability to predict the replacement of the fetal circulating red cell population with donor cells. Linear regression analysis and repeated measures analysis of variance were performed, and p-values less than 0.05 were considered significant.<\/jats:p><jats:p>\n            Results\u2003A total of 56\u2009IUTs were performed in 16 patients. There were 39 (69.6%) final KB test values collected and compared with 30 (53.6%) final Hgb electrophoresis values. Hgb electrophoresis when compared with the KB test demonstrated a significant correlation (R\n            2\u2009=\u20090.93; 95% confidence interval, 0.61\u20130.76; p\u2009&lt;\u20090.001). This same finding held true when examining the correlation at each individual IUT as well. The final KB test and Hgb electrophoresis values significantly decreased with each transfusion (p\u2009=\u20090.003). A predominance of adult donor blood was noted by the third transfusion for both laboratory indices.<\/jats:p><jats:p>\n            Conclusion\u2003Fetal Hgb electrophoresis obtained at the time of IUT demonstrates a significant correlation with the traditional KB test.<\/jats:p><jats:p>\n            Key Points\n          <\/jats:p>","DOI":"10.1055\/a-2334-6990","type":"journal-article","created":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T23:15:53Z","timestamp":1716938153000},"page":"001-005","source":"Crossref","is-referenced-by-count":0,"title":["Hemoglobin Electrophoresis versus Kleihauer\u2013Betke to Determine Bone Marrow Suppression in Fetuses Undergoing Intrauterine Transfusion"],"prefix":"10.1055","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1907-2413","authenticated-orcid":false,"given":"Alexander M.","family":"Saucedo","sequence":"additional","affiliation":[{"name":"Department of Women's Health, University of Texas at Austin, Dell Medical School, Austin, Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erin","family":"Moise","sequence":"additional","affiliation":[{"name":"Department of Women's Health, University of Texas at Austin, Dell Medical School, Austin, Texas"},{"name":"Comprehensive Fetal Care Center, Dell Children's Medical Center, Austin, Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Nwokocha","sequence":"additional","affiliation":[{"name":"Comprehensive Fetal Care Center, Dell Children's Medical Center, Austin, Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Bebbington","sequence":"additional","affiliation":[{"name":"Department of Women's Health, University of Texas at Austin, Dell Medical School, Austin, Texas"},{"name":"Comprehensive Fetal Care Center, Dell Children's Medical Center, Austin, Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5028-8426","authenticated-orcid":false,"given":"Kenneth J.","family":"Moise","sequence":"additional","affiliation":[{"name":"Department of Women's Health, University of Texas at Austin, Dell Medical School, Austin, Texas"},{"name":"Comprehensive Fetal Care Center, Dell Children's Medical Center, Austin, Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2024,6,18]]},"reference":[{"issue":"06","key":"ref1","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1016\/j.ajog.2015.01.059","article-title":"Society for Maternal-Fetal Medicine (SMFM) Clinical Guideline #8: the fetus at risk for anemia\u2014diagnosis and management","volume":"212","author":"G Mari","year":"2015","journal-title":"Am J Obstet 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