{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:38:23Z","timestamp":1790620703521,"version":"4.1.0"},"reference-count":38,"publisher":"Georg Thieme Verlag KG","issue":"07","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Drug Res (Stuttg)"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ischemic stroke remains the leading cause of death and disability, while the main\n                    mechanisms of dominant neurological damage in stroke contain oxidative stress\n                    and inflammation. Docking studies revealed a binding energy of \u2212\u20096.1\u2009kcal\/mol\n                    for AG, while the co-crystallized ligand (CCl) exhibited a binding energy of\n                    \u2212\u20097.3\u2009kcal\/mol with NOS. AG demonstrated favourable hydrogen bond interactions\n                    with amino acids ASN A:354 and ARG A:388 and hydrophobic interactions with GLU\n                    A:377. Molecular dynamics simulations throughout 100\u2009ns indicated a binding\n                    affinity of \u2212\u200927.65\u00b12.88\u2009kcal\/mol for AG, compared to \u2212\u200918.01\u00b14.02\u2009kcal\/mol for\n                    CCl. These findings suggest that AG possesses a superior binding affinity for\n                    NOS compared to CCl, thus complementing the stability of NOS at the docked\n                    site.AG has limited applications owing to its low bioavailability, poor water\n                    solubility, and high chemical and metabolic instability.The fabrication method\n                    was employed in the preparation of AGNP, SEM analysis confirmed spherical shape\n                    with size in 19.4\u00b15\u2009nm and investigated the neuroprotective effect in cerebral\n                    stroke rats induced by 30\u2009min of carotid artery occlusion followed by 4\u2009hr\n                    reperfusion, evaluated by infarction size, ROS\/RNS via GSH, MPO, NO\n                    estimationand AchE activity, and monitoring EEG function. Cortex and hippocampal\n                    histology were compared between groups. AGNP treatment significantly decreased\n                    Infarction size and increased GSH levels (p&lt;0.01**), decreased MPO\n                        (p&lt;0.01**), NO (p&lt;0.01**), AchE\n                        (p&lt;0.01**), restored to normal EEG amplitude, minimizing\n                    unsynchronized polyspikes and histological data revealed that increased\n                    pyramidal cell layer thickness and decreased apoptotic neurons in hippocampus,\n                    cortex appeared normal neurons with central large vesicular nuclei, containing\n                    one or more nucleoli in compared to AG treatment. Based on brain biochemical,\n                    histopathology reports AGNP exhibited significant cerebroprotective activity\n                    compared to AG on ischemic rats.<\/jats:p>","DOI":"10.1055\/a-2345-5396","type":"journal-article","created":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T22:52:04Z","timestamp":1720738324000},"page":"335-346","source":"Crossref","is-referenced-by-count":3,"title":["Cerebroprotective Potential of Andrographolide Nanoparticles: In\n                        silico and In vivo Investigations"],"prefix":"10.1055","volume":"74","author":[{"given":"Lakshmi Charitha","family":"Rudrala","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, SKU College of Pharmaceutical Sciences, S.\n                    K. University, Anantapur, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranadheer Reddy","family":"Challa","sequence":"additional","affiliation":[{"name":"Department of Formulations and Development, Quotient Sciences, Garnet\n                    Valley, PA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sibbala","family":"Subramanyam","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, School of Biotechnology and\n                    Pharmaceutical Sciences, Vignan\u2019s Foundation for Science, Technology &\n                    Research (Deemed to be University), Guntur, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sampath","family":"Ayyappa Gouru","sequence":"additional","affiliation":[{"name":"Aura Biosciences Inc., Boston, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gagandeep","family":"Singh","sequence":"additional","affiliation":[{"name":"Section of Microbiology, Central Ayurveda Research Institute, Jhansi,\n                    Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.V. L.","family":"Sirisha Mulukuri","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Nitte College of Pharmaceutical Sciences,\n                    Bangalore, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Praveen Kumar","family":"Pasala","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Raghavendra Institute of Pharmaceutical\n                    Education and Research, JNTUA, Anantapur, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prasanth Sree Naga Bala Krishna","family":"Dintakurthi","sequence":"additional","affiliation":[{"name":"School of Pharmacy & Technology Management, SVKM\u2019s Narsee Monjee\n                    Institute of Management Studies (NMIMS), Hyderabad, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somasekhar","family":"Gajula","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, SKU College of Pharmaceutical Sciences, S.\n                    K. University, Anantapur, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mithun","family":"Rudrapal","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, School of Biotechnology and\n                    Pharmaceutical Sciences, Vignan\u2019s Foundation for Science, Technology &\n                    Research (Deemed to be University), Guntur, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2024,7,11]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"605398","DOI":"10.3389\/fphys.2020.605398","article-title":"Blood-brain barrier damage in ischemic stroke and its regulation by endothelial\n                    mechanotransduction","volume":"11","author":"K Nian","year":"2020","journal-title":"Front Physiol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"e897","DOI":"10.1016\/S2468-2667(21)00228-0","article-title":"Temporal trend and 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