{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T02:45:41Z","timestamp":1786761941973,"version":"build-2736575974"},"reference-count":22,"publisher":"Georg Thieme Verlag KG","issue":"05","license":[{"start":{"date-parts":[[2024,9,3]],"date-time":"2024-09-03T00:00:00Z","timestamp":1725321600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004329","name":"Slovenian Research and Innovation Agency","doi-asserted-by":"crossref","award":["P1\u20130207"],"award-info":[{"award-number":["P1\u20130207"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004329","name":"Slovenian Research and Innovation Agency","doi-asserted-by":"crossref","award":["P3\u20130019"],"award-info":[{"award-number":["P3\u20130019"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004329","name":"Slovenian Research and Innovation Agency","doi-asserted-by":"crossref","award":["P4\u20130053"],"award-info":[{"award-number":["P4\u20130053"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004329","name":"Slovenian Research and Innovation Agency","doi-asserted-by":"crossref","award":["J3\u20132534"],"award-info":[{"award-number":["J3\u20132534"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004329","name":"Slovenian Research and Innovation Agency","doi-asserted-by":"crossref","award":["1000\u201322\u20130106"],"award-info":[{"award-number":["1000\u201322\u20130106"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:p>Profound and transient thrombocytopenia of functional platelets without bleeding was observed in patients envenomed by Vipera a. ammodytes (Vaa). This condition was rapidly reversed by administration of F(ab)2 fragments of immunoglobulin G targeting the whole venom, leaving platelets fully functional. To investigate the potential role of snake venom C-type lectin-like proteins (snaclecs) in this process, Vaa-snaclecs were isolated from the crude venom using different liquid chromatographies. The purity of the isolated proteins was confirmed by Edman sequencing and mass spectrometry. The antithrombotic effect was investigated by platelet agglutination and aggregation assays and blood coagulation tests. Using flow cytometry, the platelet activation and binding of Vaa-snaclecs to various platelet receptors was analyzed. Antithrombotic efficacy was tested in vivo using a mouse model of vascular injury. Two Vaa-snaclecs were purified from the venom. One of them, Vaa-snaclec-3\/2, inhibited ristocetin-induced platelet agglutination. It is a covalent heterodimer of Vaa-snaclec-3 (\u03b1-subunit) and Vaa-snaclec-2 (\u03b2-subunit). Our results suggest that Vaa-snaclec-3\/2 induces platelet agglutination and consequently thrombocytopenia by binding to the platelet receptor glycoprotein Ib. Essentially, no platelet activation was observed in this process. In vivo, Vaa-snaclec-3\/2 was able to protect the mouse from ferric chloride-induced carotid artery thrombosis, revealing its applicative potential in interventional angiology and cardiology.<\/jats:p>","DOI":"10.1055\/a-2408-9375","type":"journal-article","created":{"date-parts":[[2024,9,3]],"date-time":"2024-09-03T23:40:23Z","timestamp":1725406823000},"page":"484-491","source":"Crossref","is-referenced-by-count":5,"title":["Reversible Thrombocytopenia of Functional Platelets after Nose-Horned Viper Envenomation is Induced by a Snaclec"],"prefix":"10.1055","volume":"125","author":[{"given":"Mojca","family":"Dobaja Borak","sequence":"first","affiliation":[{"name":"Centre for Clinical Toxicology and Pharmacology, University Medical Centre Ljubljana, Ljubljana, Slovenia"},{"name":"Doctoral School, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrijana","family":"Leonardi","sequence":"first","affiliation":[{"name":"Department of Molecular and Biomedical Sciences, Jo\u017eef Stefan Institute, Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kity","family":"Po\u017eek","sequence":"additional","affiliation":[{"name":"Doctoral School, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia"},{"name":"Department of Molecular and Biomedical Sciences, Jo\u017eef Stefan Institute, Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarina","family":"Reber\u0161ek","sequence":"additional","affiliation":[{"name":"Department of Haematology, University Medical Centre Ljubljana, Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"Podgornik","sequence":"additional","affiliation":[{"name":"Department of Haematology, University Medical Centre Ljubljana, Ljubljana, Slovenia"},{"name":"Department of Clinical Biochemistry, Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alja\u017e","family":"Pirnat","sequence":"additional","affiliation":[{"name":"Division of Paediatrics, University Medical Centre Ljubljana, Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alenka","family":"Trampu\u0161 Bakija","sequence":"additional","affiliation":[{"name":"Division of Paediatrics, University Medical Centre Ljubljana, Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Kranjc Brezar","sequence":"additional","affiliation":[{"name":"Department of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toma\u017e","family":"Trobec","sequence":"additional","affiliation":[{"name":"Institute 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