{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T05:26:40Z","timestamp":1741325200348,"version":"3.38.0"},"reference-count":32,"publisher":"Georg Thieme Verlag KG","issue":"05","funder":[{"name":"Labcorp"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Perinatol"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The relationship between fetal fraction and birth weight in twin gestations is poorly understood. This study aimed to investigate the relationship between first-trimester cell-free DNA (cfDNA) fetal fraction and birth weight &lt;10th percentile in twin gestations.<\/jats:p><jats:p>This is a planned secondary analysis of the Twin cfDNA Study, a 17-center retrospective cohort of twin pregnancies screened for aneuploidy using cfDNA in the first trimester from December 2011 to February 2022, excluding those with positive screen results for chromosomal aneuploidy. cfDNA testing was performed by a single laboratory using massively parallel sequencing. Baseline characteristics and birth weight of pregnancies with normal fetal fraction were compared with those with low (&lt;5%) and high (&gt;95%) fetal fraction using univariable analyses and multivariable regression.<\/jats:p><jats:p>A total of 1,041 twin pregnancies were included. Chronic hypertension, elevated body mass index, and self-identified Black race were associated with fetal fraction &lt;5th percentile. There was no difference in median fetal fraction between those with birth weight &lt;10th percentile in at least one twin (median [interquartile range (IQR)] fetal fraction: 12.2% [9.8, 14.8] vs. those with normal birth weight (\u226510th percentile) in both twins (median [IQR] fetal fraction: 12.3% [9.7, 15.2] for normal birth weight, p\u2009=\u20090.49). There was no association between high or low fetal fraction and birth weight &lt;10th percentile for one (p\u2009=\u20090.45) or both (p\u2009=\u20090.81) twins, and there was no association between high or low fetal fraction and birth weight &lt;5th percentile for one (p\u2009=\u20090.44) or both (p\u2009=\u20090.74) twins. The results were unchanged after adjustment for potential confounders.<\/jats:p><jats:p>In this large cohort, there was no association between the extremes of cfDNA fetal fraction and birth weight &lt;10th percentile, suggesting that first-trimester fetal fraction may not predict impaired fetal growth in twin gestations.<\/jats:p>","DOI":"10.1055\/a-2413-2353","type":"journal-article","created":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T23:02:41Z","timestamp":1726095761000},"page":"605-611","source":"Crossref","is-referenced-by-count":0,"title":["First-Trimester Cell-Free DNA Fetal Fraction and Birth Weight in Twin Pregnancies"],"prefix":"10.1055","volume":"42","clinical-trial-number":[{"clinical-trial-number":"nct04488393","registry":"10.18810\/clinical-trials-gov"}],"author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8337-3085","authenticated-orcid":false,"given":"Molly R.","family":"Siegel","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Massachusetts General Hospital, Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaitlyn","family":"James","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Massachusetts General Hospital, Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryann","family":"Bromley","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Massachusetts General Hospital, Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathanael C.","family":"Koelper","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Women's Health Clinical Research Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen T.","family":"Chasen","sequence":"additional","affiliation":[{"name":"Division of Maternal-Fetal Medicine, Weill Cornell Medical College, New York, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurie","family":"Griffin","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, The Warren Alpert Medical School at Brown University, Providence, Rhode Island"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashley S.","family":"Roman","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, NYU Grossman School of Medicine, New York, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3514-1183","authenticated-orcid":false,"given":"Meghana","family":"Limaye","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, NYU Grossman School of Medicine, New York, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Ranzini","sequence":"additional","affiliation":[{"name":"Division of Maternal-Fetal Medicine, MetroHealth, Cleveland, Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caitlin","family":"Clifford","sequence":"additional","affiliation":[{"name":"Division of Maternal-Fetal Medicine, University of Michigan Health, Ann Arbor, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph R.","family":"Biggio","sequence":"additional","affiliation":[{"name":"Division of Maternal-Fetal Medicine, Ochsner Health, New Orleans, Louisiana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akila","family":"Subramaniam","sequence":"additional","affiliation":[{"name":"Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, University of Alabama at Birmingham Heersink School of Medicine, Birmingham, Alabama"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela R.","family":"Seasely","sequence":"additional","affiliation":[{"name":"Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, University of Alabama at Birmingham Heersink School of Medicine, Birmingham, Alabama"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica M.","family":"Page","sequence":"additional","affiliation":[{"name":"Division of Maternal-Fetal Medicine, Intermountain Health, Salt Lake City, Utah"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara S.","family":"Nicholas","sequence":"additional","affiliation":[{"name":"Axia Women's Health Main Line Perinatal Associates, Wynnewood, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jay","family":"Idler","sequence":"additional","affiliation":[{"name":"Division of Maternal-Fetal Medicine, Allegheny Health Network, Pittsburgh, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rashmi","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, UCLA, Los Angeles, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raj","family":"Shree","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graham","family":"McLennan","sequence":"additional","affiliation":[{"name":"Clinical Affairs, Sequenom Inc, LabCorp, San Diego, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorraine","family":"Dugoff","sequence":"additional","affiliation":[{"name":"Division of Reproductive Genetics, Department of Obstetrics and Gynecology, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania"},{"name":"Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"on behalf of the Twin cfDNA Study Consortium","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2024,10,1]]},"reference":[{"issue":"05","key":"ref1","doi-asserted-by":"crossref","first-page":"A1","DOI":"10.1530\/REP-19-0591","article-title":"Preimplantation genetic testing: non-invasive prenatal testing for aneuploidy, copy-number variants and single-gene disorders","volume":"160","author":"J Shaw","year":"2020","journal-title":"Reproduction"},{"issue":"17","key":"ref2","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1056\/NEJMoa1407349","article-title":"Cell-free DNA analysis for noninvasive examination of trisomy","volume":"372","author":"M E Norton","year":"2015","journal-title":"N Engl J 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