{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T06:50:31Z","timestamp":1753685431242,"version":"3.40.3"},"reference-count":24,"publisher":"Georg Thieme Verlag KG","issue":"03\/04","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Drug Res (Stuttg)"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To investigate the effect of 1\u03b1,25(OH)2D3 on hepatic\n                        stellate cells and the mechanism of the TGF-\u03b21\/Smad signaling pathway.<\/jats:p><jats:p>LX2 cells were treated with TGF-\u03b21 and different concentrations of\n                            1\u03b1,25(OH)2D3. Cell proliferation was assessed\n                        using the CCK8 assay to determine the optimal concentration of\n                            1\u03b1,25(OH)2D3 activity. The cell cycle and\n                        apoptotic rates were evaluated using flow cytometry. The expressions of\n                        Samd2, Samd3, Samd4, and Samd7 was assessed by western blotting, whereas the\n                        expression of MMP1, MMP13, and TIMP-1 was detected by qPCR.<\/jats:p><jats:p>Compared with the control group, the 1\u03b1,25(OH)2D3 group\n                        had a higher apoptotic rate of LX2 cells, the cell cycle was blocked from\n                        the G1 stage to the S stage, the expressions of Samd2, Samd7, MMP1, and\n                        MMP13 increased, while the expressions of Samd3, Samd4, and TIMP-1\n                        decreased.<\/jats:p><jats:p>1\u03b1,25(OH)2D3 inhibits hepatic stellate cell activation\n                        and exerts anti-hepatic fibrosis effects by downregulating the expression of\n                        Samd3, Samd4, TIMP-1 and upregulating the expression of Samd2, Samd4, MMP1,\n                        and MMP13.<\/jats:p>","DOI":"10.1055\/a-2463-5530","type":"journal-article","created":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T23:52:03Z","timestamp":1736985123000},"page":"94-99","source":"Crossref","is-referenced-by-count":1,"title":["1\u03b1,25(OH)2D3 Regulates the TGF-\u03b21\/Samd\n                    Signaling Pathway Inhibition of Hepatic Stellate Cell Activation"],"prefix":"10.1055","volume":"75","author":[{"given":"Yihan","family":"Zhao","sequence":"first","affiliation":[{"name":"Xi\u2019an Eighth Hospital, Xi\u2019an, China"},{"name":"Second Clinical College, Shaanxi University of Traditional Chinese\n                    Medicine, Xianyang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianghao","family":"Fan","sequence":"first","affiliation":[{"name":"Xi\u2019an Eighth Hospital, Xi\u2019an, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Wang","sequence":"additional","affiliation":[{"name":"Xi\u2019an Eighth Hospital, Xi\u2019an, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Wan","sequence":"additional","affiliation":[{"name":"Xi\u2019an Eighth Hospital, Xi\u2019an, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiyan","family":"Ma","sequence":"additional","affiliation":[{"name":"Xi\u2019an Eighth Hospital, Xi\u2019an, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoying","family":"Sha","sequence":"additional","affiliation":[{"name":"Xi\u2019an Eighth Hospital, Xi\u2019an, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongli","family":"Wang","sequence":"additional","affiliation":[{"name":"Xi\u2019an Eighth Hospital, Xi\u2019an, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2025,1,15]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1038\/s41392-022-01070-3","article-title":"Targeting fibrosis, mechanisms and cilinical trials","volume":"7","author":"M Zhao","year":"2022","journal-title":"Signal Transduct Target Ther"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/s41575-018-0055-0","article-title":"The changing epidemiology of liver diseases in the Asia-Pacific region","volume":"16","author":"MC S Wong","year":"2019","journal-title":"Nat Rev Gastroenterol Hepatol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1053\/j.gastro.2014.11.039","article-title":"Nonalcoholic steatohepatitis is the second leading etiology of liver disease\n                    among adults awaiting liver transplantation in the United States","volume":"148","author":"R J Wong","year":"2015","journal-title":"Gastroenterology"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1016\/j.jhep.2018.05.011","article-title":"EASL HEPAHEALTH Steering Committee. 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