{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T22:39:16Z","timestamp":1792276756681,"version":"4.4.0"},"reference-count":35,"publisher":"Georg Thieme Verlag KG","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Portal vein system-specific risk factors contributing to portal vein thrombosis in cirrhosis are poorly investigated.<\/jats:p>\n                  <jats:p>This study aimed to quantify contact system and intrinsic pathway activation in the peripheral compared to portal venous blood in patients with decompensated cirrhosis.<\/jats:p>\n                  <jats:p>Adult patients with cirrhosis undergoing transjugular intrahepatic portosystemic shunt underwent simultaneous blood sampling from a peripheral vein and the portal vein. Complexes of serine proteases with their respective inhibitors were measured by ELISA to quantify contact system (PKa:C1-INH [plasma kallikrein:C1-esterase inhibitor] and FXIIa:C1-INH) and intrinsic pathway activation (FXIa:C1-INH, FXIa:\u03b11at [\u03b1-1 antitrypsin], FXIa:AT [antithrombin], and FIXa:AT).<\/jats:p>\n                  <jats:p>Twenty patients with cirrhosis (mean age 55\u2009\u00b1\u20097 years, M\u2009=\u200958%, Child\u2013Pugh A\/B\/C 6\/11\/3) and 25 healthy controls (mean age 45\u2009\u00b1\u200912 years, M\u2009=\u200960%) were enrolled. The etiology of cirrhosis was primarily alcohol abuse, followed by chronic viral infection. Log-transformed peripheral levels of all the complexes were significantly higher in patients compared with controls. While levels of PKa:C1-INH, FXIIa:C1-INH, FXIa:C1-INH and FXIa:\u03b11at were similar in peripheral and portal venous blood in cirrhotic patients, FXIa:AT and FIXa:AT levels were significantly higher in portal blood (p\u2009=\u20090.013 and 0.011, respectively). FXIa:C1-INH significantly correlated with both contact system complexes (FXIIa:C1-INH and PKa:C1-INH) and with FIX:AT.<\/jats:p>\n                  <jats:p>Markers of contact system and intrinsic pathway activation in the systemic circulation were significantly higher in cirrhosis versus controls. Complexes of FXIa and FIXa with AT were significantly higher in the portal than in peripheral plasma in cirrhosis, possibly indicating a unique heparin-like effect in portal venous blood.<\/jats:p>","DOI":"10.1055\/a-2507-2449","type":"journal-article","created":{"date-parts":[[2024,12,24]],"date-time":"2024-12-24T19:00:21Z","timestamp":1735066821000},"page":"1195-1204","source":"Crossref","is-referenced-by-count":1,"title":["Activation of the Contact System and Intrinsic Pathway in Peripheral and Portal Venous Circulations in Liver Cirrhosis"],"prefix":"10.1055","volume":"125","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0377-3741","authenticated-orcid":false,"given":"Elena","family":"Campello","sequence":"additional","affiliation":[{"name":"Internal Medicine 1, Thrombotic and Hemorrhagic Diseases Unit, Department of Medicine, Padova University Hospital, Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Zanetto","sequence":"additional","affiliation":[{"name":"Gastroenterology and Multivisceral Transplant Unit, Department of Surgery, Oncology and Gastroenterology, Padova University Hospital, Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuriy","family":"Prokopenko","sequence":"additional","affiliation":[{"name":"Division of Hematology and Blood Research Center, Department of Medicine, University of North Carolina, Chapel Hill, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton","family":"Ilich","sequence":"additional","affiliation":[{"name":"Division of Hematology and Blood Research Center, Department of Medicine, University of North Carolina, Chapel Hill, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chatphatai","family":"Moonla","sequence":"additional","affiliation":[{"name":"Division of Hematology and Blood Research Center, Department of Medicine, University of North Carolina, Chapel Hill, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiana","family":"Bulato","sequence":"additional","affiliation":[{"name":"Internal Medicine 1, Thrombotic and Hemorrhagic Diseases Unit, Department of Medicine, Padova University Hospital, Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serena","family":"Toffanin","sequence":"additional","affiliation":[{"name":"Internal Medicine 1, Thrombotic and Hemorrhagic Diseases Unit, Department of Medicine, Padova University Hospital, Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Shalaby","sequence":"additional","affiliation":[{"name":"Gastroenterology and Multivisceral Transplant Unit, Department of Surgery, Oncology and Gastroenterology, Padova University Hospital, Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romilda","family":"Cardin","sequence":"additional","affiliation":[{"name":"Gastroenterology and Multivisceral Transplant Unit, Department of Surgery, Oncology and Gastroenterology, Padova University Hospital, Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulio","family":"Barbiero","sequence":"additional","affiliation":[{"name":"Radiology Unit, Department of Medicine, Padova University Hospital, Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Gavasso","sequence":"additional","affiliation":[{"name":"Internal Medicine 1, Thrombotic and Hemorrhagic Diseases Unit, Department of Medicine, Padova University Hospital, Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nigel S.","family":"Key","sequence":"additional","affiliation":[{"name":"Division of Hematology and Blood Research Center, Department of Medicine, University of North Carolina, Chapel Hill, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Senzolo","sequence":"additional","affiliation":[{"name":"Gastroenterology and Multivisceral Transplant Unit, Department of Surgery, Oncology and Gastroenterology, Padova University Hospital, Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Simioni","sequence":"additional","affiliation":[{"name":"Internal Medicine 1, Thrombotic and Hemorrhagic Diseases Unit, Department of Medicine, Padova University Hospital, Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2025,1,16]]},"reference":[{"issue":"03","key":"ref1","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.jhep.2009.12.001","article-title":"Normal to increased thrombin generation in patients undergoing liver transplantation despite prolonged conventional coagulation 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