{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T12:12:15Z","timestamp":1775304735251,"version":"3.50.1"},"reference-count":41,"publisher":"Georg Thieme Verlag KG","issue":"04","funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"crossref","award":["13N16290 (MaZurKA)"],"award-info":[{"award-number":["13N16290 (MaZurKA)"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["TRR240"],"award-info":[{"award-number":["TRR240"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ministerium f\u00fcr Innovation, Wissenschaft und Forschung des Landes Nordrhein-Westfalen"},{"name":"Center for Protein Diagnostics (ProDi) grants"},{"name":"Protein Research Unit Ruhr within Europe"},{"DOI":"10.13039\/501100014690","name":"Ministerium f\u00fcr Kultur und Wissenschaft des Landes Nordrhein-Westfalen","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100014690","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Regierender B\u00fcrgermeister von Berlin"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Comprehensive characterization of platelets requires various functional assays and analytical techniques, including omics disciplines, each demanding a separate aliquot of the given sample. Consequently, sample material for each assay is often highly limited, necessitating the downscaling of methods to work with just a few micrograms of platelet protein.<\/jats:p>\n                  <jats:p>Here, we present a novel sample preparation platform for proteomics analysis using only 3\u2009\u03bcg of purified platelet protein, corresponding to 2\u2009\u00d7\u2009106 platelets, which can be obtained from approximately 2 to 8\u2009\u03bcL of blood from a healthy individual (1.5\u2009\u00d7\u2009105\u20134.5\u2009\u00d7\u2009105 platelets\/\u03bcL) or approximately 100\u2009\u03bcL of blood from a patient with severe thrombocytopenia (&lt;2\u2009\u00d7\u2009104 platelets\/\u00b5L).<\/jats:p>\n                  <jats:p>Using this platform, we detected a significant fraction of key players in the platelet activation cascade and, most importantly, identified 36 clinically relevant platelet disease markers even with a non-state-of-the art instrument. This makes LC-MS-based proteomics a highly attractive alternative to conventional assays, which often require milliliters of blood. Our platform transitions from our previously established 96-well proteomics workflow (PF96), which has been successfully employed in numerous platelet proteomics studies, into the 384-well format. This transition is accompanied by (1) a more than two-fold increase in sensitivity, (2) improved reproducibility, (3) a four-fold increase in throughput, allowing 1,536 samples to be processed per lab worker per week, and (4) reduced sample preparation costs.<\/jats:p>\n                  <jats:p>Thus, LC-MS-based platelet proteomics offers a compelling alternative to immunoaffinity assays (which depend on antibody availability and quality), as well as to genomic assays (which can only reveal genotypes). In summary, in conjunction with recent advances in LC-MS instrumentation, our platform represents a highly valuable tool for rapid phenotyping of platelets in research with extraordinary potential for future employment in companion or routine diagnostics.<\/jats:p>","DOI":"10.1055\/a-2516-1812","type":"journal-article","created":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T18:58:03Z","timestamp":1736967483000},"page":"394-404","source":"Crossref","is-referenced-by-count":1,"title":["Middle-Throughput LC-MS-Based Platelet Proteomics with Minute Sample Amounts Using Semiautomated Positive Pressure FASP in 384-Well Format"],"prefix":"10.1055","volume":"126","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7758-0301","authenticated-orcid":false,"given":"Stefan","family":"Loroch","sequence":"additional","affiliation":[{"name":"Medical Proteome-Center (MPC), Medical Faculty, Ruhr-University Bochum, Bochum, Germany"},{"name":"Medical Proteome Analysis, Center for Protein Diagnostics (PRODI), Ruhr-University Bochum, Bochum, Germany"},{"name":"Fa. Dr. Loroch\/QC|MS, Biomedizin Zentrum, Dortmund, Dortmund, Germany"},{"name":"Department of Bioanalytics, Leibniz-Institute for Analytical Sciences \u2013 ISAS \u2013 e.V., Dortmund Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eleftherios","family":"Panagiotidis","sequence":"additional","affiliation":[{"name":"Department of Bioanalytics, Leibniz-Institute for Analytical Sciences \u2013 ISAS \u2013 e.V., Dortmund Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristoffer","family":"Klewe","sequence":"additional","affiliation":[{"name":"Fa. Dr. Loroch\/QC|MS, Biomedizin Zentrum, Dortmund, Dortmund, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9179-4851","authenticated-orcid":false,"given":"Frauke","family":"Swieringa","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, The Netherlands"},{"name":"Synapse Research Institute, Platelet Pathophysiology, Maastricht, the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2848-5121","authenticated-orcid":false,"given":"Johan W.M.","family":"Heemskerk","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, The Netherlands"},{"name":"Synapse Research Institute, Platelet Pathophysiology, Maastricht, the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan-Paul","family":"Lerch","sequence":"additional","affiliation":[{"name":"Fakult\u00e4t f\u00fcr Mathematik, Universit\u00e4t Bielefeld, Bielefeld, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8343-7336","authenticated-orcid":false,"given":"Andreas","family":"Greinacher","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Immunologie und Transfusionsmedizin, Universit\u00e4tsmedizin Greifswald, Greifswald, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Walter","sequence":"additional","affiliation":[{"name":"Center for Thrombosis and Hemostasis (CTH), Univeristy Medical Center Mainz, Mainz, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerstin","family":"Jurk","sequence":"additional","affiliation":[{"name":"Center for Thrombosis and Hemostasis (CTH), Univeristy Medical Center Mainz, Mainz, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobias","family":"John","sequence":"additional","affiliation":[{"name":"Medical Proteome-Center (MPC), Medical Faculty, Ruhr-University Bochum, Bochum, Germany"},{"name":"Medical Proteome Analysis, Center for Protein Diagnostics (PRODI), Ruhr-University Bochum, Bochum, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katalin","family":"Barkovits","sequence":"additional","affiliation":[{"name":"Medical Proteome-Center (MPC), Medical Faculty, Ruhr-University Bochum, Bochum, Germany"},{"name":"Medical Proteome Analysis, Center for Protein Diagnostics (PRODI), Ruhr-University Bochum, Bochum, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Dandekar","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics, Biocenter, University of W\u00fcrzburg, W\u00fcrzburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katrin","family":"Marcus","sequence":"additional","affiliation":[{"name":"Medical Proteome-Center (MPC), Medical Faculty, Ruhr-University Bochum, Bochum, Germany"},{"name":"Medical Proteome Analysis, Center for Protein Diagnostics (PRODI), Ruhr-University Bochum, Bochum, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johannes","family":"Balkenhol","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics, Biocenter, University of W\u00fcrzburg, W\u00fcrzburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2025,3,6]]},"reference":[{"issue":"07","key":"ref1","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1161\/CIRCRESAHA.114.301598","article-title":"What can proteomics tell us about platelets?","volume":"114","author":"J M Burkhart","year":"2014","journal-title":"Circ 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