{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T22:09:32Z","timestamp":1785881372616,"version":"3.56.0"},"reference-count":37,"publisher":"Georg Thieme Verlag KG","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Horm Metab Res"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Lipoprotein apheresis (LA) is often the last option to adequately reduce\n                    lipoproteins in patients with familial hypercholesterolemia and lipoprotein (a)\n                    hyperlipidemia. Characterized by mild side effects, it is now the most effective\n                    method of preventing major cardiovascular events (CVEs). This benefit is due not\n                    only to the lowering of lipoprotein levels, but probably also to many other\n                    pleiotropic effects that have been extensively described in the literature.\n                    These include the reduction of inflammatory signaling substances, fibrinogen,\n                    plasminogen or components of the oxidative stress response. Here, we performed a\n                    proteomic analysis of 12 patients treated with therapeutic apheresis using two\n                    different pore size filters to quantify the effect on age-related plasma\n                    proteins. This study showed that important proteins such as \u03b1-2-macroglobulin,\n                    apolipoprotein C-III, complement C1s subcomponent, C4b-binding protein alpha\n                    chain, CD5 antigen-like and pregnancy zone protein, whose role in numerous aging\n                    processes has been well described, were significantly reduced by apheresis\n                    treatment. We conclude that therapeutic apheresis may be a promising approach to\n                    reduce these age-related proteins and that these treatments may become an\n                    essential part of managing cardiovascular risk in an aging population.<\/jats:p>","DOI":"10.1055\/a-2563-1187","type":"journal-article","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T19:08:46Z","timestamp":1744744126000},"page":"626-631","source":"Crossref","is-referenced-by-count":4,"title":["Proteomic Profiling of Age-Related Proteins Following Extracorporeal\n                    Apheresis"],"prefix":"10.1055","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6795-2303","authenticated-orcid":false,"given":"Romy","family":"Walther","sequence":"additional","affiliation":[{"name":"Department and Outpatient Department of Medicine III,\n                    University Hospital Carl Gustav Carus, Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5834-8320","authenticated-orcid":false,"given":"Bhawana","family":"Singh","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute, Imperial College\n                    London, London, United Kingdom of Great Britain and Northern\n                    Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoke","family":"Yin","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute, Imperial College\n                    London, London, United Kingdom of Great Britain and Northern\n                    Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Mavberg","sequence":"additional","affiliation":[{"name":"Ayus Medical Group, Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"M\u00fccke","sequence":"additional","affiliation":[{"name":"INUSpheresis Center Basel, Ayus Medical Group, Basel,\n                    Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman","family":"Rodionov","sequence":"additional","affiliation":[{"name":"Department and Outpatient Department of Medicine III,\n                    University Hospital Carl Gustav Carus, Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud","family":"Babir","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Harefield Hospital,\n                    Harefield, United Kingdom of Great Britain and Northern Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Mayr","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute, Imperial College\n                    London, London, United Kingdom of Great Britain and Northern\n                    Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan R.","family":"Bornstein","sequence":"additional","affiliation":[{"name":"Department and Outpatient Department of Medicine III,\n                    University Hospital Carl Gustav Carus, Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2025,4,15]]},"reference":[{"key":"ref1","first-page":"345","article-title":"Impact of aging on cardiovascular diseases: from chronological observation to\n                    biological insights: JACC Fam Ser","volume":"4","author":"D Zhao","year":"2024","journal-title":"JACC Asia"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1016\/j.ajpath.2011.10.024","article-title":"Age-dependent changes in the cerebrospinal fluid proteome by slow off-rate\n                    modified aptamer array","volume":"180","author":"G S Baird","year":"2012","journal-title":"Am J Pathol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"e17213","DOI":"10.1371\/journal.pone.0017213","article-title":"Age-related differences in plasma proteins: how plasma proteins change from\n                    neonates to adults","volume":"6","author":"V Ignjatovic","year":"2011","journal-title":"PloS One"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"e13325","DOI":"10.1111\/acel.13325","article-title":"Proteomics in aging research: a roadmap to clinical, translational research","volume":"20","author":"R Moaddel","year":"2021","journal-title":"Aging Cell"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1038\/s41586-023-06802-1","article-title":"Organ aging signatures in the plasma proteome track health and disease","volume":"624","author":"H S Oh","year":"2023","journal-title":"Nature"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1038\/s41576-022-00511-7","article-title":"Measuring biological age using omics data","volume":"23","author":"J Rutledge","year":"2022","journal-title":"Nat Rev Genet"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"R741","DOI":"10.1016\/j.cub.2012.07.024","article-title":"Ageing as a risk factor for disease","volume":"22","author":"T Niccoli","year":"2012","journal-title":"Curr Biol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.atherosclerosissup.2015.02.018","article-title":"Effects of different lipoprotein apheresis methods on serum protein levels","volume":"18","author":"U Julius","year":"2015","journal-title":"Atheroscler Suppl"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.atherosclerosissup.2017.05.029","article-title":"Long-term follow-up of circulating oxidative stress markers in patients\n                    undergoing lipoprotein apheresis by Direct Adsorption of Lipids (DALI)","volume":"30","author":"S Kopprasch","year":"2017","journal-title":"Atheroscler Suppl"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.atherosclerosis.2004.01.017","article-title":"Long-term reduction of C-reactive protein concentration by regular LDL\n                    apheresis","volume":"174","author":"C Otto","year":"2004","journal-title":"Atherosclerosis"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S1567-5688(09)71806-9","article-title":"Acute effects of lipid apheresis on human serum lipidome","volume":"10","author":"S Tselmin","year":"2009","journal-title":"Atheroscler Suppl"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1055\/a-1776-7943","article-title":"Precision medicine approach for cardiometabolic risk factors in therapeutic\n                    apheresis","volume":"54","author":"X Yin","year":"2022","journal-title":"Horm Metab Res"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1007\/0-387-29362-0_23","volume-title":"limma: Linear models for microarray data. In: Gentleman R, Carey VJ, Huber W,\n                    Irizarry RA, Dudoit S (eds). Bioinformatics and computational biology solutions\n                    using R and bioconductor","author":"G K Smyth","year":"2005"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.atherosclerosis.2019.08.014","article-title":"2019 ESC\/EAS guidelines for the management of dyslipidaemias: Lipid modification\n                    to reduce cardiovascular risk","volume":"290","year":"2019","journal-title":"Atherosclerosis"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1007\/s11883-021-00914-7","article-title":"ANGPTL3 and apolipoprotein C-III as novel lipid-lowering targets","volume":"23","author":"I Akoumianakis","year":"2021","journal-title":"Curr Atheroscler Rep"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1038\/s41590-019-0548-1","article-title":"Apolipoprotein C3 induces inflammation and organ damage by alternative\n                    inflammasome activation","volume":"21","author":"S Zewinger","year":"2020","journal-title":"Nat Immunol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1802","DOI":"10.1016\/j.bbadis.2018.08.008","article-title":"Endothelial cell senescence in aging-related vascular dysfunction","volume":"1865","author":"G Jia","year":"2019","journal-title":"Biochim Biophys Acta Mol Basis Dis"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1055\/s-0038-1676817","article-title":"Apolipoprotein C-III strongly correlates with activated factor VII\u2013anti-thrombin\n                    complex: an additional link between plasma lipids and coagulation","volume":"119","author":"N Martinelli","year":"2019","journal-title":"Thromb Haemost"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1770","DOI":"10.1056\/NEJMoa2402309","article-title":"Olezarsen for hypertriglyceridemia in patients at high cardiovascular risk","volume":"390","author":"B A Bergmark","year":"2024","journal-title":"N Eng J Med"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1007\/s11883-023-01140-z","article-title":"Updates in drug treatment of severe hypertriglyceridemia","volume":"25","author":"I Gouni-Berthold","year":"2023","journal-title":"Curr Atheroscler Rep"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"100475","DOI":"10.1016\/j.jlr.2023.100475","article-title":"Apolipoprotein C3: form begets function","volume":"65","author":"K E Bornfeldt","year":"2024","journal-title":"J Lipid Res"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/BF02591638","article-title":"Determination of serum levels of complement component C4b-binding protein:\n                    influence of age and inflammation","volume":"21","author":"S M Marcovina","year":"1991","journal-title":"Int J Clin Lab Res"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1182\/blood.V78.2.357.357","article-title":"C4b-binding protein exacerbates the host response to escherichia coli","volume":"78","author":"F Taylor","year":"1991","journal-title":"Blood"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"2511","DOI":"10.1074\/jbc.M102293200","article-title":"The \u03b1-chains of C4b-binding protein mediate complex formation with low density\n                    lipoprotein receptor-related protein","volume":"277","author":"E Westein","year":"2002","journal-title":"J Biol Chem"},{"key":"ref25","first-page":"451","article-title":"C4b-binding protein deposition is induced in diseased aortic heart valves,\n                    coinciding with C3d","volume":"24","author":"M J Dikhoff","year":"2015","journal-title":"J Heart Valve Dis"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1136\/ard.2006.052118","article-title":"Expression of the peptide C4b-binding protein beta in the arthritic joint","volume":"65","author":"O S\u00e1nchez-Pernaute","year":"2006","journal-title":"Ann Rheum Dis"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"e2886","DOI":"10.1371\/journal.pone.0002886","article-title":"C4b-binding protein is present in affected areas of myocardial infarction during\n                    the acute inflammatory phase and covers a larger area than C3","volume":"3","author":"L A Trouw","year":"2008","journal-title":"PloS one"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.exger.2003.11.012","article-title":"mRNA level of alpha-2-macroglobulin as an aging biomarker of human fibroblasts\n                    in culture","volume":"39","author":"H Ma","year":"2004","journal-title":"Exp Gerontol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1177\/03913988221086597","article-title":"Rheopheresis: a narrative review","volume":"45","author":"G Kosmadakis","year":"2022","journal-title":"Int J Artificial Organs"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1046\/j.1526-0968.2000.004005348.x","article-title":"Rheopheresis: rheologic, functional, and structural aspects","volume":"4","author":"R Klingel","year":"2000","journal-title":"Therap Apheresis"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"3570","DOI":"10.1021\/acs.jproteome.3c00403","article-title":"High-level secretion of pregnancy zone protein is a novel biomarker of DNA\n                    damage-induced senescence and promotes spontaneous senescence","volume":"22","author":"Z Hu","year":"2023","journal-title":"J Proteome Res"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1164\/rccm.201812-2351OC","article-title":"Pregnancy zone protein is associated with airway infection, neutrophil\n                    extracellular trap formation, and disease severity in bronchiectasis","volume":"200","author":"S Finch","year":"2019","journal-title":"Am J Respir Crit Care Med"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1189\/jlb.3RU0215-074R","article-title":"AIM\/CD5L: a key protein in the control of immune homeostasis and inflammatory\n                    disease","volume":"98","author":"L Sanjurjo","year":"2015","journal-title":"J Leukocyte Biol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"22690","DOI":"10.18632\/aging.203615","article-title":"Circulating CD5L is associated with cardiovascular events and all-cause\n                    mortality in individuals with chronic kidney disease","volume":"13","author":"E Castelblanco","year":"2021","journal-title":"Aging (Albany NY)"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.atherosclerosissup.2017.05.032","article-title":"Pleiotropic effects of regular lipoprotein-apheresis","volume":"30","author":"H Sinzinger","year":"2017","journal-title":"Atheroscler Suppl"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1111\/1744-9987.12056","article-title":"Proteomic analysis of proteins eliminated by low-density lipoprotein\n                    apheresis","volume":"18","author":"Y Yuasa","year":"2014","journal-title":"Therap Apheresis Dialysis"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"2762","DOI":"10.1038\/s41598-024-51782-5","article-title":"Lipoprotein apheresis affects the concentration of extracellular vesicles in\n                    patients with elevated lipoprotein (a)","volume":"14","author":"J Marl\u0119ga-Linert","year":"2024","journal-title":"Sci Rep"}],"container-title":["Hormone and Metabolic Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-2563-1187.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T23:46:07Z","timestamp":1763768767000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-2563-1187"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,15]]},"references-count":37,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,11,21]]},"published-print":{"date-parts":[[2025,11]]}},"URL":"https:\/\/doi.org\/10.1055\/a-2563-1187","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["0018-5043","1439-4286"],"issn-type":[{"value":"0018-5043","type":"print"},{"value":"1439-4286","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,15]]}}}