{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,17]],"date-time":"2025-05-17T04:16:16Z","timestamp":1747455376442,"version":"3.40.5"},"reference-count":24,"publisher":"Georg Thieme Verlag KG","issue":"CP","license":[{"start":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T00:00:00Z","timestamp":1747353600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Endosc Int Open"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Matched therapy based on comprehensive genomic profiling is a potential treatment option for patients with inoperable pancreatic cancer; however, the optimal method for obtaining tissue samples suitable for comprehensive genomic profiling using endoscopic ultrasound-guided tissue acquisition remains unclear. This study aimed to determine the optimal endoscopic ultrasound-guided tissue acquisition method to obtain samples for comprehensive genomic profiling.<\/jats:p><jats:p>This retrospective study included 86 consecutive patients with pancreatic cancer who underwent comprehensive genomic profiling using FoundationOne CDx (Foundation Medicine Inc.) and endoscopic ultrasound-guided tissue acquisition between June 2019 and January 2023. Macroscopic visible core length was measured using on-site macroscopic evaluation in all patients. Foundation Medicine Inc. reported analysis results categorized as passed (successful FoundationOne CDx), qualified, or failed. We investigated factors predicting successful FoundationOne CDx treatment.<\/jats:p><jats:p>Needles sized 22, 20, and 19 gauge were used in 63, one, and 23 patients, respectively.\n        The stylet slow-pull and suction techniques were performed in 43 and 41 patients,\n        respectively. Median total macroscopic visible core length in the formalin-fixed\n        paraffin-embedded blocks subjected to FoundationOne CDx was 41 mm. The success rate for\n        FoundationOne CDx was 66%. Multiple linear regression analysis revealed that macroscopic\n        visible core length independently affected successful FoundationOne CDx (P = 0.0019).<\/jats:p><jats:p>In tissue specimens obtained using endoscopic ultrasound-guided tissue acquisition, macroscopic visible core length can be associated with an appropriate sample for FoundationOne CDx.<\/jats:p>","DOI":"10.1055\/a-2593-4172","type":"journal-article","created":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T23:27:36Z","timestamp":1747438056000},"source":"Crossref","is-referenced-by-count":0,"special_numbering":"S","title":["Benefits of macroscopic on-site evaluation in endoscopic ultrasound-guided tissue acquisition for comprehensive genomic profiling"],"prefix":"10.1055","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0514-4452","authenticated-orcid":false,"given":"Junya","family":"Sato","sequence":"additional","affiliation":[{"name":"Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan"},{"name":"Gastroenterology, St Marianna University School of Medicine, Kawasaki, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6719-4466","authenticated-orcid":false,"given":"Hirotoshi","family":"Ishiwatari","sequence":"additional","affiliation":[{"name":"Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuma","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Sapporo Medical University, Sapporo, Japan"},{"name":"Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroki","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7202-0178","authenticated-orcid":false,"given":"Takuya","family":"Doi","sequence":"additional","affiliation":[{"name":"Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Yamamura","sequence":"additional","affiliation":[{"name":"Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazunori","family":"Takada","sequence":"additional","affiliation":[{"name":"Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoichi","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, 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Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kinichi","family":"Hotta","sequence":"additional","affiliation":[{"name":"Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Ono","sequence":"additional","affiliation":[{"name":"Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2025,5,16]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"7","DOI":"10.3322\/caac.21551","article-title":"Cancer statistics, 2019","volume":"69","author":"RL Siegel","year":"2019","journal-title":"CA Cancer J Clin"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"2913","DOI":"10.1158\/0008-5472.CAN-14-0155","article-title":"Projecting cancer incidence and deaths to 2030: the unexpected burden of thyroid, liver, and pancreas cancers in the 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