{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T14:34:58Z","timestamp":1781620498247,"version":"3.54.5"},"reference-count":30,"publisher":"Georg Thieme Verlag KG","issue":"05","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tierarztl Prax Ausg G Grosstiere Nutztiere"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>Zusammenfassung<\/jats:title>\n                  <jats:p>Ziel dieser Studie war es, die in-vitro-Empfindlichkeit von\n                        Mastitis-assoziierten Escherichia (E.) coli-Isolaten gegen\u00fcber\n                        ausgew\u00e4hlten und in der Praxis einsetzbaren veterin\u00e4rmedizinisch relevanten\n                        Wirkstoffen zu untersuchen, um eventuelle Alternativen zu den\n                        Cephalosporinen der 3. und 4. Generation bzw. den Fluorchinolonen\n                        aufzuzeigen.<\/jats:p>\n                  <jats:p>Dazu wurden im Milchlabor des klinischen Zentrums f\u00fcr Wiederk\u00e4uer- und\n                        Kamelidenmedizin der Vetmeduni Vienna 154 E. coli-Isolate aus\n                        Viertelgemelksproben von K\u00fchen mit Mastitis sowohl mittels\n                        Agardiffusionstest (ADT) als auch mittels Bouillon-Mikrodilutionsverfahren\n                        (MICRONAUT-S Mastitis 3, Bruker Corporation, Billerica, Massachusetts, USA)\n                        untersucht. Zur Identifizierung von ESBL\/AmpC \u03b2-Laktamase positiven E.\n                            coli-Isolate wurde ein spezielles Testverfahren (D68C MASTDISCS,\n                        Mast Group Ltd, UK) verwendet.<\/jats:p>\n                  <jats:p>Beim Agardiffusionstest sowie beim Bouillon-Mikrodilutionstest konnte eine\n                        hohe in-vitro-Wirksamkeit von Chinolonen\/Fluorchinolonen,\n                        Cephalosporinen der 3. Generation und von Amoxicillin\/Clavulans\u00e4ure\n                        gegen\u00fcber den getesteten E. coli-Isolaten nach den CLSI-Standards\n                        nachgewiesen werden. Hohe Resistenzraten wurden hingegen gegen\u00fcber\n                        Trimethoprim\/Sulfamethoxazol (24,0%), Tetracyclin (17,5%) und Ampicillin\n                        (ADT 19,5%) ermittelt. Drei\u00dfig von 154 Isolaten wiesen Mehrfachresistenzen\n                        auf. Unter den Reserveantibiotika (Ciprofloxacin, Cefoperazon) waren 11\n                        gegen\u00fcber Ciprofloxacin, 6 gegen\u00fcber Cefoperazon resistent. Vier von 30\n                        mehrfachresistenten Isolaten zeigten eine ESBL-Aktivit\u00e4t, keines der Isolate\n                        zeigte eine AmpC-Aktivit\u00e4t.<\/jats:p>\n                  <jats:p>E. coli ist einer der wichtigsten umweltassoziierten Mastitis-Erreger\n                        weltweit. Die antimikrobielle Therapie bei schweren klinischen\n                        Mastitisverl\u00e4ufen wird h\u00e4ufig parenteral mit Wirkstoffen durchgef\u00fchrt, die\n                        in der Veterin\u00e4rmedizin als Reserveantibiotika (Cephalosporine der 3. und 4.\n                        Generation und Fluorchinolone) klassifiziert sind. Auf Basis der\n                            in-vitro-Ergebnisse stellt Amoxicillin\/Clavulans\u00e4ure eine\n                        alternative antimikrobielle Therapiem\u00f6glichkeit zu den High Priority\n                        Critically Important Antimicrobials (Cephalosporine der 3. und 4. Generation\n                        bzw. Fluorchinolone) f\u00fcr eine Score 3 E. coli-Mastitis dar.<\/jats:p>","DOI":"10.1055\/a-2638-4534","type":"journal-article","created":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T22:37:59Z","timestamp":1760567879000},"page":"289-300","source":"Crossref","is-referenced-by-count":1,"title":["Resistenzverhalten boviner Mastitis-assoziierter Escherichia\n                        coli-Isolate gegen\u00fcber ausgew\u00e4hlten antimikrobiellen Wirkstoffen in\n                    \u00d6sterreich"],"prefix":"10.1055","volume":"53","author":[{"given":"Susanna","family":"Piechl","sequence":"additional","affiliation":[{"name":"Klinisches Department f\u00fcr Nutztiere und Sicherheit von\n                    Lebensmittelsystemen, Klinisches Zentrum f\u00fcr Wiederk\u00e4uer- und Kamelidenmedizin,\n                    Veterin\u00e4rmedizinische Universit\u00e4t Wien, \u00d6sterreich"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Verena","family":"Urbantke","sequence":"additional","affiliation":[{"name":"Klinisches Department f\u00fcr Nutztiere und Sicherheit von\n                    Lebensmittelsystemen, Klinisches Zentrum f\u00fcr Wiederk\u00e4uer- und Kamelidenmedizin,\n                    Veterin\u00e4rmedizinische Universit\u00e4t Wien, \u00d6sterreich"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Spergser","sequence":"additional","affiliation":[{"name":"Department f\u00fcr Biologische Wissenschaften und Pathobiologie,\n                    Veterin\u00e4rmedizinische Universit\u00e4t Wien, \u00d6sterreich"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Wehrend","sequence":"additional","affiliation":[{"name":"Tierklinik f\u00fcr Reproduktionsmedizin und Neugeborenenkunde,\n                    Justus-Liebig-Universit\u00e4t Gie\u00dfen, Deutschland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Wittek","sequence":"additional","affiliation":[{"name":"Klinisches Department f\u00fcr Nutztiere und Sicherheit von\n                    Lebensmittelsystemen, Klinisches Zentrum f\u00fcr Wiederk\u00e4uer- und Kamelidenmedizin,\n                    Veterin\u00e4rmedizinische Universit\u00e4t Wien, \u00d6sterreich"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Baumgartner","sequence":"additional","affiliation":[{"name":"Klinisches Department f\u00fcr Nutztiere und Sicherheit von\n                    Lebensmittelsystemen, Klinisches Zentrum f\u00fcr Wiederk\u00e4uer- und Kamelidenmedizin,\n                    Veterin\u00e4rmedizinische Universit\u00e4t Wien, \u00d6sterreich"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2025,10,15]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1080\/00480169.2011.547165","article-title":"Economic aspects of mastitis: new developments","volume":"59","author":"H Hogeveen","year":"2011","journal-title":"New Zealand Veterinary Journal"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0136387","article-title":"Genomic and Phenomic Study of Mammary Pathogenic Escherichia coli","author":"S E Blum","year":"2015","journal-title":"PloS ONE"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2016-12334","article-title":"Incidence of clinical mastitis and distribution of pathogens on large Chinese\n                    dairy farms","author":"J Gao","year":"2017","journal-title":"Journal of Dairy Science"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvfa.2012.03.009","article-title":"Managing environmental mastitis. 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