{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T03:45:37Z","timestamp":1784346337914,"version":"3.55.0"},"reference-count":43,"publisher":"Georg Thieme Verlag KG","issue":"03","license":[{"start":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T00:00:00Z","timestamp":1753401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"crossref","award":["ANR-13-BSV1-0026,ANR-15-RHU-0004"],"award-info":[{"award-number":["ANR-13-BSV1-0026,ANR-15-RHU-0004"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Aneurysms of the thoracic (TAA) and abdominal aorta (AAA) have different pathophysiological mechanisms. AAA has an intraluminal thrombus, while TAA does not. This suggests a prothrombotic phenotype in AAA, probably at the level of vascular smooth muscle cells (SMCs) known to express tissue factor (TF).<\/jats:p>\n                  <jats:p>To explore the TF-dependent thrombin generation in SMCs in AAA compared with TAA and healthy aorta (HA) and the underlying mechanisms contributing to a procoagulant phenotype.<\/jats:p>\n                  <jats:p>Human HA, AAA, or TAA tissues and corresponding SMC primary cultures were used to analyze SMC-supported thrombin generation and TF expression.<\/jats:p>\n                  <jats:p>In the absence of added TF, thrombin generation was increased at the surface of SMCs from AAA compared with TAA and HA, indicating a cellular procoagulant phenotype, which is transmitted through mitosis. Phosphatidylserine exposure was increased at the surface of SMCs from AAA. As expected, reactive oxygen species generation and the proinflammatory cytokine TNF-\u03b1 were increased in SMCs from AAA. Overexpression of protease-activated receptor 2 and nuclear translocation of NF-\u03baB p65 in SMCs and tissue from AAA triggered increased TF gene expression. Higher active basal TF expression was also observed in SMCs from AAA, which was inhibited by BAY 11\u20137082 (pharmacological inhibitor of I\u03baK) and GB83 (pharmacological inhibitor of PAR-2).<\/jats:p>\n                  <jats:p>We demonstrated a PAR-2-mediated activation of the canonical NF-\u03baB pathway, which triggers TF transcription in AAA. This procoagulant profile is transmitted from tissue to primary SMC cultures and through numerous passages, which can maintain thrombus formation.<\/jats:p>","DOI":"10.1055\/a-2665-2510","type":"journal-article","created":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T22:42:39Z","timestamp":1753483359000},"page":"319-331","source":"Crossref","is-referenced-by-count":1,"title":["NF-\u03baB-Mediated Upregulation of Tissue Factor Contributes to the Procoagulant Phenotype of Smooth Muscle Cells from Abdominal Aorta Aneurysm in Human"],"prefix":"10.1055","volume":"126","author":[{"given":"Veronique","family":"Regnault","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lorraine, Inserm, DCAC, Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melusine","family":"Didelot","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lorraine, Inserm, DCAC, Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronique","family":"Ollivier","sequence":"additional","affiliation":[{"name":"Inserm UMR_S 1148, CHU X. Bichat, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecile","family":"Lakomy","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lorraine, Inserm, DCAC, Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy","family":"Lagrange","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lorraine, Inserm, DCAC, Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huguette","family":"Louis","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lorraine, Inserm, DCAC, Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecile V.","family":"Denis","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, Inserm, HITh, Le Kremlin-Bic\u00eatre, France"},{"name":"CHRU de Nancy, 54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serguei","family":"Malikov","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lorraine, Inserm, DCAC, Nancy, France"},{"name":"Department of Vascular and Endovascular Surgery, Nancy University Hospital, Vandoeuvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Lacolley","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lorraine, Inserm, DCAC, Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Baptiste","family":"Michel","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lorraine, Inserm, DCAC, Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2025,8,12]]},"reference":[{"issue":"09","key":"ref1","doi-asserted-by":"crossref","first-page":"2254","DOI":"10.1161\/ATVBAHA.117.310126","article-title":"Abdominal aortic aneurysms growth is associated with high concentrations of plasma proteins in the intraluminal thrombus and diseased arterial tissue","volume":"38","author":"C Behr Andersen","year":"2018","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"06","key":"ref2","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1161\/ATVBAHA.106.137851","article-title":"Topological determinants and consequences of adventitial responses to arterial wall injury","volume":"27","author":"J B Michel","year":"2007","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"01","key":"ref3","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1038\/s41572-018-0030-7","article-title":"Abdominal aortic aneurysms","volume":"4","author":"N Sakalihasan","year":"2018","journal-title":"Nat Rev Dis Primers"},{"issue":"01","key":"ref4","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1160\/TH13-08-0721","article-title":"From tissue iron retention to low systemic haemoglobin levels, new pathophysiological biomarkers of human abdominal aortic aneurysm","volume":"112","author":"R Martinez-Pinna","year":"2014","journal-title":"Thromb Haemost"},{"issue":"18","key":"ref5","doi-asserted-by":"crossref","first-page":"6756","DOI":"10.3390\/ijms21186756","article-title":"Red blood cells and hemoglobin in human atherosclerosis and related arterial diseases","volume":"21","author":"J B Michel","year":"2020","journal-title":"Int J Mol Sci"},{"issue":"05","key":"ref6","doi-asserted-by":"crossref","first-page":"2073","DOI":"10.1172\/JCI117202","article-title":"Endothelial cells stimulated with tumor necrosis factor-alpha express varying amounts of tissue factor resulting in inhomogenous fibrin deposition in a native blood flow system. 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